Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA

Miao, Xinxing; Fang, Qianqian; Xiao, Xiang; Han, Dong; Wu, Renfei; Yan, Jun; Nie, Baoqing; Liu, Jian

doi:10.3389/fmolb.2021.676065

Cited by 8 publications

(3 citation statements)

References 40 publications

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“…The content of single-base mutated ctDNA sequences increased in diffuse intrinsic pontine gliomas (DIPGs). Miao et al ( 60 ) reported a method by combining cyclic enzyme DNA amplification technology and gold nanoparticles@silicon (AuNPs@Si) assisted SERS technique, as shown in Figure 8 . They designed an oligonucleotide probe folded into a stem ring hairpin, labeled with a cyanine dye (Cy5) at the 5′ end.…”

Section: Application Of Responsive Sers Probes In Biomedical Fieldsmentioning

confidence: 99%

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A review of clinical use of surface-enhanced Raman scattering-based biosensing for glioma

Yang,

Zhang,

et al. 2024

Front. Neurol.

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Glioma is the most common malignant tumor of the nervous system in recent centuries, and the incidence rate of glioma is increasing year by year. Its invasive growth and malignant biological behaviors make it one of the most challenging malignant tumors. Maximizing the resection range (EOR) while minimizing the impact on normal brain tissue is crucial for patient prognosis. Changes in metabolites produced by tumor cells and their microenvironments might be important indicators. As a powerful spectroscopic technique, surface-enhanced Raman scattering (SERS) has many advantages, including ultra-high sensitivity, high specificity, and non-invasive features, which allow SERS technology to be widely applied in biomedicine, especially in the differential diagnosis of malignant tumor tissues. This review first introduced the clinical use of responsive SERS probes. Next, the sensing mechanisms of microenvironment-responsive SERS probes were summarized. Finally, the biomedical applications of these responsive SERS probes were listed in four sections, detecting tumor boundaries due to the changes of pH-responsive SERS probes, SERS probes to guide tumor resection, SERS for liquid biopsy to achieve early diagnosis of tumors, and the application of free-label SERS technology to detect fresh glioma specimens. Finally, the challenges and prospects of responsive SERS detections were summarized for clinical use.

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Section: Application Of Responsive Sers Probes In Biomedical Fieldsmentioning

confidence: 99%

“…(C) SERS spectra of testing Cy5 collected from 50 random spots on the AuNPs@Si substrate in a single assay. (D) Averaged SERS intensities at three peaks from 50 random spots, respectively ( 60 ). Copyright 2021 Frontiers Media SA.…”

Section: Application Of Responsive Sers Probes In Biomedical Fieldsmentioning

confidence: 99%

A review of clinical use of surface-enhanced Raman scattering-based biosensing for glioma

Yang,

Zhang,

et al. 2024

Front. Neurol.

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“…DNA nanotechnology takes advantage of DNA structures and functions that have not yet been used in the natural world [ 1 , 2 ], which makes DNA not only function as a genetic information carrier [ 3 , 4 , 5 ], but also act as a role of programmable material [ 6 , 7 ]. Owing to the distinct advantages of programmability and predictability, DNA molecules have been used for fabricating two- or three-dimensional nanostructures by a precise sequence design [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Active Self-Assembly of Ladder-Shaped DNA Carrier for Drug Delivery

et al. 2023

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With the advent of nanotechnology, DNA molecules have been transformed from solely genetic information carriers to multifunctional materials, showing a tremendous potential for drug delivery and disease diagnosis. In drug delivery systems, DNA is used as a building material to construct drug carriers through a variety of DNA self-assembly methods, which can integrate multiple functions to complete in vivo and in situ tasks. In this study, ladder-shaped drug carriers are developed for drug delivery on the basis of a DNA nanoladder. We first demonstrate the overall structure of the nanoladder, in which a nick is added into each rung of the nanoladder to endow the nanoladder with the ability to incorporate a drug loading site. The structure is designed to counteract the decrement of stability caused by the nick and investigated in different conditions to gain insight into the properties of the nicked DNA nanoladders. As a proof of concept, we fix the biotin in every other nick as a loading site and assemble the protein (streptavidin) on the loading site to demonstrate the feasibility of the drug-carrying function. The protein can be fixed stably and can be extended to different biological and chemical drugs by altering the drug loading site. We believe this design approach will be a novel addition to the toolbox of DNA nanotechnology, and it will be useful for versatile applications such as in bioimaging, biosensing, and targeted therapy.

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