2022
DOI: 10.3389/fnagi.2022.870957
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Integrating Lipidomics and Transcriptomics Reveals the Crosstalk Between Oxidative Stress and Neuroinflammation in Central Nervous System Demyelination

Abstract: Multiple sclerosis (MS) is an incurable and progressive neurodegenerative disease that affects more than 2.5 million people worldwide and brings tremendous economic pressures to society. However, the pathophysiology of MS is still not fully elucidated, and there is no effective treatment. Demyelination is thought to be the primary pathophysiological alteration in MS, and our previous study found abnormal lipid metabolism in the demyelinated corpus callosum. Growing evidence indicates that central nervous syste… Show more

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Cited by 26 publications
(15 citation statements)
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“…Lipid profiling, identification, and quantification. Lipid profiling, identification, and quantification were implemented as described below, following the protocol from a previous study 70 . In more detail, the lipid profiling was first conducted with a LC-ESI-MS/MS system, which combines the ultra performance liquid chromatography (UPLC) using the SCIEX ExionLC AD system (Danaher Corporation, America) and the tandem mass spectrometry (MS/MS) using the SCIEX QTRAP ® LC-MS/ MS system (Danaher Corporation, America).…”
Section: Lipidomic Datamentioning
confidence: 99%
“…Lipid profiling, identification, and quantification. Lipid profiling, identification, and quantification were implemented as described below, following the protocol from a previous study 70 . In more detail, the lipid profiling was first conducted with a LC-ESI-MS/MS system, which combines the ultra performance liquid chromatography (UPLC) using the SCIEX ExionLC AD system (Danaher Corporation, America) and the tandem mass spectrometry (MS/MS) using the SCIEX QTRAP ® LC-MS/ MS system (Danaher Corporation, America).…”
Section: Lipidomic Datamentioning
confidence: 99%
“…The lipidomics analysis method was similar to the previously reported ( Zhao et al, 2022 ). We first used unsupervised principal component analysis (PCA) to observe the overall distribution across samples as well as the stability of the entire analysis process ( Supplementary Figure S2A ), and then supervised partial least squares analysis (PLS-DA) and orthogonal partial least squares analysis (OPLS-DA) were used to distinguish the overall differences in metabolic profiles between groups and find the differential metabolites between groups ( Supplementary Figures S2C-D ).…”
Section: Methodsmentioning
confidence: 90%
“…We also used the CopyKAT R package ( 29 ) to analyze the CNV characteristics of CAFs clusters and distinguish between tumor cells and normal ones. Finally, we performed Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis on the marker genes using the clusterProfiler package ( 30 ).…”
Section: Methodsmentioning
confidence: 99%