Integrating network pharmacology and transcriptomic validation to investigate the efficacy and mechanism of Mufangji decoction preventing lung cancer

Gao, Fan; Niu, Yuju; Sun, Luyao; Li, Wenwen; Xia, Haojie; Zhang, Yaru; Geng, Shengnan; Guo, Zhenzhen; Lin, Hailong; Du, Gangjun

doi:10.1016/j.jep.2022.115573

Cited by 10 publications

(5 citation statements)

References 51 publications

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“…Colorectal cancer is the third most commonly diagnosed malignancy worldwide, with significant geographic heterogeneity in incidence and mortality . The combination of network pharmacology and molecular docking technology is gradually forming a systematic paradigm, providing an unprecedented opportunity for the systematic study of traditional medicinal plants in cancer, and becoming a Frontier research field in drug discovery and development. − It has been reported that Mufangji decoction, a famous traditional Chinese medicine formula, can treat lung cancer by multiple pathways, multiple targets, and multiple components based on network pharmacology and experimental validation . Gao et al also reported that aloin protects against gastric cancer by regulating the PI3K-AKT signaling pathway through integrating network pharmacology and experimental verification .…”

Section: Discussionmentioning

confidence: 99%

“… 35 − 37 It has been reported that Mufangji decoction, a famous traditional Chinese medicine formula, can treat lung cancer by multiple pathways, multiple targets, and multiple components based on network pharmacology and experimental validation. 38 Gao et al also reported that aloin protects against gastric cancer by regulating the PI3K-AKT signaling pathway through integrating network pharmacology and experimental verification. 39 Cur is an important traditional medicinal plant that has gained popularity due to its beneficial health properties, including antitumor activity.…”

Section: Discussionmentioning

confidence: 99%

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Exploration of the Molecular Mechanism of Curcuma aromatica Salisb’s Anticolorectal Cancer Activity via the Integrative Approach of Network Pharmacology and Experimental Validation

Yin,

Tan,

Jiang

2024

ACS Omega

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Curcuma aromatica Salisb (Cur), a well-known herbal medicine, has a wide spectrum of anti-inflammatory, anticarcinogenic, and antioxidant activities. However, the roles of its active compounds and potential mechanisms in colorectal cancer remain unknown. This research utilized network pharmacology and experimental validation to explore the possible mechanisms by which Cur protects against colorectal cancer. The active compounds of Cur and related genes for colorectal cancer were obtained from public databases. The DrugBank database was used to search for anticolorectal cancer drugs licensed through the FDA and their targets, and a "drug-component-target" relationship network was created using the Cytoscape program. The String database produced the PPI network. The ability of these active ingredients to bind to core targets was confirmed by molecular docking using AutoDock Vina. Cell and animal experiments were then carried out. A total of 274 targets were obtained from Cur, 49 of which were potential therapeutic targets. Four key targets, PTGS2, AKT1, TP53, and estrogen receptor 1 (ESR1), were screened via the PPI network and the FDA drug-target network. Molecular docking results revealed that Cur had strong binding abilities to these targets. In vivo and in vitro experiments demonstrated that Cur suppressed the development of colorectal cancer by regulating its targets (PTGS2, AKT1, TP53, and ESR1), which play crucial roles in promoting apoptosis and suppressing cell proliferation, migration, and invasion. Collectively, Cur protects against colorectal cancer by regulating the AKT1/PTGS2/ESR1 and P53 pathways, which lays the groundwork for further research and clinical applications of Cur in colorectal cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exploration of the Molecular Mechanism of Curcuma aromatica Salisb’s Anticolorectal Cancer Activity via the Integrative Approach of Network Pharmacology and Experimental Validation

Yin,

Tan,

Jiang

2024

ACS Omega

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“…Generations of medical doctors believe that "phlegm" is asthma recurrent attacks of the "long-standing root". In addition, Chinese medicine believes that the onset of asthma is also closely related to "blood stasis" [5,6] . Asthma patients often have shortness of breath and wheezing, limb cyanosis, cyanosis of the lips, facial blood vessels, tongue reddish-red or dark purple and other phlegm stasis and gas obstruction.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Jiawei Sanzi Yangqin Decoction in the Treatment of Asthma Based on Network Pharmacology and Molecular Docking

2024

IJFM

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This study investigated the effectiveness of Jiawei Sanzi Yangqin Decoction (JSYD) in asthma treatment, utilizing network pharmacology and molecular docking methods. The key components of JSYD and their targets were pinpointed using the TCMSP database, while specific asthma-related targets were collated from the GeneGards and Disgenet databases. We constructed interaction networks between JSYD components and biological targets using Cytoscape 3.9.1 and the STRING database. This was followed by gene ontology and pathway analysis via Metascape. Selected targets and ingredients underwent molecular docking, were analyzed using AutoDockTools Vina, and visualized with Pymol. Our findings emphasize that the constituents of JSYD, especially kaempferol, interact with various molecules, such as IL-6, and affect key pathways, including Th17 cell differentiation. This research highlights the comprehensive potential of JSYD in asthma management and paves the way for more indepth studies.

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“…The previous year, F. Gao et al reported the antitumor activity and mechanism of Mufangji decoction (MFJD), of which SIN is a component in preventing lung cancer. The results showed that SIN (3 μM) enhanced the phagocytosis of neutrophils and increased MPO and ROS to promote neutrophil-related immune surveillance [ 37 ]. Other studies revealed that SIN inhibited the proliferation of SK-Hep1 and induced apoptosis, decreasing PCNA, PI3K p85α, AKT1, BcL-2, and pro-caspase-3/-9 and increasing cleaved caspase-9/-3 in tumor tissue.…”

Section: Antitumor Activitymentioning

confidence: 99%

Bioactivities and Mechanisms of Action of Sinomenine and Its Derivatives: A Comprehensive Review

Hou,

Huang,

Huang

et al. 2024

Molecules

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Sinomenine, an isoquinoline alkaloid extracted from the roots and stems of Sinomenium acutum, has been extensively studied for its derivatives as bioactive agents. This review concentrates on the research advancements in the biological activities and action mechanisms of sinomenine-related compounds until November 2023. The findings indicate a broad spectrum of pharmacological effects, including antitumor, anti-inflammation, neuroprotection, and immunosuppressive properties. These compounds are notably effective against breast, lung, liver, and prostate cancers, exhibiting IC50 values of approximately 121.4 nM against PC-3 and DU-145 cells, primarily through the PI3K/Akt/mTOR, NF-κB, MAPK, and JAK/STAT signaling pathways. Additionally, they manifest anti-inflammatory and analgesic effects predominantly via the NF-κB, MAPK, and Nrf2 signaling pathways. Utilized in treating rheumatic arthritis, these alkaloids also play a significant role in cardiovascular and cerebrovascular protection, as well as organ protection through the NF-κB, Nrf2, MAPK, and PI3K/Akt/mTOR signaling pathways. This review concludes with perspectives and insights on this topic, highlighting the potential of sinomenine-related compounds in clinical applications and the development of medications derived from natural products.

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Integrating network pharmacology and transcriptomic validation to investigate the efficacy and mechanism of Mufangji decoction preventing lung cancer

Cited by 10 publications

References 51 publications

Exploration of the Molecular Mechanism of Curcuma aromatica Salisb’s Anticolorectal Cancer Activity via the Integrative Approach of Network Pharmacology and Experimental Validation

Exploration of the Molecular Mechanism of Curcuma aromatica Salisb’s Anticolorectal Cancer Activity via the Integrative Approach of Network Pharmacology and Experimental Validation

Mechanism of Jiawei Sanzi Yangqin Decoction in the Treatment of Asthma Based on Network Pharmacology and Molecular Docking

Bioactivities and Mechanisms of Action of Sinomenine and Its Derivatives: A Comprehensive Review

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