Integrating transcriptional profiling with network-based methodologies for revealing molecular mechanisms of traditional Chinese medicine

Huang, Lusheng; Jiang, Lihua; Kuang, Qifan; Li, M; Wen, Zhining; Li, He

doi:10.13172/2052-7845-1-1-519

Cited by 1 publication

(1 citation statement)

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“…Although the expression of several genes has been shown to mediate the effects of A. cinnamomea in colorectal cancer, more evidence of the pharmacological mechanisms at the molecular level remains necessary for better understanding. Microarray technology and the associated bioinformatic tools have become widely used methods to investigate the molecular mechanisms of traditional Chinese medicines 21 , 22 . According to microarray gene expression profiles, Si-Wu-Tang, a traditional Chinese medicinal formula utilized for menstrual discomfort relief, was identified as a Nrf2 activator and suggested to be used as a nontoxic chemopreventive agent 23 .…”

Section: Introductionmentioning

confidence: 99%

Antrodia cinnamomea induces autophagic cell death via the CHOP/TRB3/Akt/mTOR pathway in colorectal cancer cells

Tsai

Chung²,

Lee

2018

Sci Rep

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Antrodia cinnamomea, a well-known traditional medicine used in Taiwan, is a potent anticancer drug for colorectal cancer, but the upstream molecular mechanism of its anticancer effects remains unclear. In this study, A. cinnamomea extracts showed cytotoxicity in HCT116, HT29, SW480, Caco-2 and, Colo205 colorectal cancer cells. Whole-genome expression profiling of A. cinnamomea extracts in HCT116 cells was performed. A. cinnamomea extracts upregulated the expression of the endoplasmic reticulum stress marker CHOP and its downstream gene TRB3. Moreover, dephosphorylation of Akt and mTOR as well as autophagic cell death were observed. Gene expression and autophagic cell death were reversed by the knockdown of CHOP and TRB3. Autophagy inhibition but not apoptosis inhibition reversed A. cinnamomea-induced cell death. Finally, we demonstrated that A. cinnamomea extracts significantly suppressed HCT116 tumour growth in nude mice. Our findings suggest that autophagic cell death via the CHOP/TRB3/Akt/mTOR pathway may represent a new mechanism of anti-colorectal cancer action by A. cinnamomea. A. cinnamomea is a new CHOP activator and potential drug that can be used in colorectal cancer treatment.

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Section: Introductionmentioning

confidence: 99%

Antrodia cinnamomea induces autophagic cell death via the CHOP/TRB3/Akt/mTOR pathway in colorectal cancer cells

Tsai

Chung²,

Lee

2018

Sci Rep

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Integrating transcriptional profiling with network-based methodologies for revealing molecular mechanisms of traditional Chinese medicine

Abstract: can monitor TCM-induced changes of whole genomics and be used to identify the differentially expressed genes for further interpretation of TCM efficacy. Further research is needed to improve our methods and understanding of TCM.

Cited by 1 publication

References 34 publications

Antrodia cinnamomea induces autophagic cell death via the CHOP/TRB3/Akt/mTOR pathway in colorectal cancer cells

Antrodia cinnamomea induces autophagic cell death via the CHOP/TRB3/Akt/mTOR pathway in colorectal cancer cells

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