2022
DOI: 10.1016/j.scr.2022.102746
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Integration-free induced pluripotent stem cell line derived from a 62-years-old male donor with APOE-epsilon4/epsilon4 alleles

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Cited by 2 publications
(1 citation statement)
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“…PBMCs (3 × 10 5 cells) were transduced with the reprogramming factors OCT3/4, SOX2, c-MYC, and KLF4 using a CytoTuneTM-iPS 2.0 Sendai reprogramming kit (Thermo Fisher Scientific, USA) according to the manufacturer's instructions [ 25 ]. The trilineage differentiation potential and chromosomal aberrations in the PBMC-derived iPSCs were tested using a STEMdiff™ trilineage differentiation kit (StemCell Technologies) and by standard G-band karyotyping, respectively [ 26 ]. Karyotyping was performed by the Department of Clinical Human Genetic Center (Fourth Hospital of Shijiazhuang, China).…”
Section: Methodsmentioning
confidence: 99%
“…PBMCs (3 × 10 5 cells) were transduced with the reprogramming factors OCT3/4, SOX2, c-MYC, and KLF4 using a CytoTuneTM-iPS 2.0 Sendai reprogramming kit (Thermo Fisher Scientific, USA) according to the manufacturer's instructions [ 25 ]. The trilineage differentiation potential and chromosomal aberrations in the PBMC-derived iPSCs were tested using a STEMdiff™ trilineage differentiation kit (StemCell Technologies) and by standard G-band karyotyping, respectively [ 26 ]. Karyotyping was performed by the Department of Clinical Human Genetic Center (Fourth Hospital of Shijiazhuang, China).…”
Section: Methodsmentioning
confidence: 99%