Integration of genetic engineering into conventional rice breeding programs for the next generation

Sagehashi, Yoshiyuki; Ikegaya, Tomohito; Fujino, Kenji

doi:10.1007/s10681-022-03102-z

Cited by 3 publications

(1 citation statement)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low-temperature stress, especially during the booting stage, causes irreversible pollen sterility, resulting in lower yields (Hayase et al 1969;Satake 1976). Rice, native to the tropics and subtropics, can now be grown at high latitudes above 40°N (Sagehashi et al 2022). Acquisition of cold tolerance traits during the booting stage is important for rice acclimation to low temperatures at high latitudes (Li et al 2021;Liu et al 2018;Zhang et al 2017).…”

Section: Introductionmentioning

confidence: 99%

The anther transcriptome of cold-tolerant rice cultivars is largely insensitive to temperature changes

Yamamori,

Ishiguro,

Ogasawara

et al. 2024

Preprint

View full text Add to dashboard Cite

Many studies of stress tolerance in plants have characterized genes that show differences among a small number of lines with clearly distinct tolerance or sensitivity to the given stress. From the few cloned genes, it is difficult to genetically interpret intermediate tolerance or susceptibility levels and explain the complexity of stress responses and tolerance. In this study, we explored the changes in the transcriptome of anthers from 13 rice lines with different cold tolerance grown under control conditions or exposed to 4 days of cold stress to look for correlations between cold tolerance at the booting stage and expression levels. When examining the overall expression patterns in anthers at low temperature, the cold-tolerant lines tended to have relatively few highly expressed genes, and the expression levels of ribosome-related genes tended to be lower in cold-tolerant lines than in cold-sensitive lines. Importantly, we observed these different expression patterns between the cold-tolerant and -sensitive lines regardless of whether cold stress had been applied. Minimal expression changes under cold stress tended to be characteristic of the cold-tolerant lines, especially in repetitive sequences. We also identified unknown genes whose expression was cold responsive and common to all the lines studied. We conclude that rice lines whose transcriptome remains constant or insensitive in response to cold stress are more tolerant to low-temperature exposure during the booting stage than rice lines with more widespread expression changes.

show abstract

Section: Introductionmentioning

confidence: 99%

The anther transcriptome of cold-tolerant rice cultivars is largely insensitive to temperature changes

Yamamori,

Ishiguro,

Ogasawara

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Sensitivity of genotypically diverse rice varieties to radiation and the related changes to antioxidant enzyme activities

Yanting,

Bingkui,

Mengchao

et al. 2023

International Journal of Radiation Biology

View full text Add to dashboard Cite

Exogenous Brassinolide Ameliorates the Adverse Effects of Gamma Radiation Stress and Increases the Survival Rate of Rice Seedlings by Modulating Antioxidant Metabolism

Lu,

Wang,

Zhang

et al. 2024

IJMS

View full text Add to dashboard Cite

Gamma irradiation-based mutant creation is one of the most important methods for rice plant mutagenesis breeding and molecular biology research. Although median lethal dose irradiation severely damages rice seedlings, applying brassinolide (BR) can increase the survival rate of irradiated seedlings. In this study, we investigated the effects of soaking seeds in solutions containing different BR concentrations (0.001, 0.01, 0.1, 1.0, and 5.0 μmol/L) and then spraying the resulting seedlings twice with 0.1 μmol/L BR. The combined BR treatments markedly decreased the superoxide anion (O2•−), hydrogen peroxide (H2O2), and malondialdehyde contents but increased the chlorophyll content. An appropriate BR treatment of gamma-irradiated samples substantially increased the activities of the antioxidant enzymes superoxide dismutase, peroxidase, and ascorbate peroxidase as well as the proline, ascorbic acid, and glutathione contents in rice seedling shoots. The BR treatment also promoted the growth of seedlings derived from irradiated seeds and increased the shoot and root fresh and dry weights. Most notably, soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BR significantly increased the final seedling survival rate and decreased mutant loss. The study results suggest that exogenous BR treatments can protect rice seedlings from gamma irradiation stress by enhancing antioxidant metabolism.

show abstract

Integration of genetic engineering into conventional rice breeding programs for the next generation

Cited by 3 publications

References 76 publications

The anther transcriptome of cold-tolerant rice cultivars is largely insensitive to temperature changes

The anther transcriptome of cold-tolerant rice cultivars is largely insensitive to temperature changes

Sensitivity of genotypically diverse rice varieties to radiation and the related changes to antioxidant enzyme activities

Exogenous Brassinolide Ameliorates the Adverse Effects of Gamma Radiation Stress and Increases the Survival Rate of Rice Seedlings by Modulating Antioxidant Metabolism

Contact Info

Product

Resources

About