Integration of metabolomics and transcriptomics analyses reveals sphingosine-1-phosphate-mediated S1PR2/PI3K/Akt pathway involved in Talaromyces marneffei infection of macrophages

Sirt5 improves cardiomyocytes fatty acid metabolism and ameliorates cardiac lipotoxicity in diabetic cardiomyopathy via CPT2 de-succinylation

“…Subsequently, the supernatant was centrifuged again and transferred to the liner of the corresponding injection bottle for on-board analysis. The sample extracts were analyzed using a LC-ESI-MS/MS system (UPLC, ExionLC AD, https://sciex.com.cn/ ; MS, QTRAP® System, https://sciex.com/ ) [ 33 ]. LIT and triple quadrupole scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (QTRAP), QTRAP® LC-MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (Sciex) [ 33 ].…”

“…The sample extracts were analyzed using a LC-ESI-MS/MS system (UPLC, ExionLC AD, https://sciex.com.cn/ ; MS, QTRAP® System, https://sciex.com/ ) [ 33 ]. LIT and triple quadrupole scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (QTRAP), QTRAP® LC-MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (Sciex) [ 33 ]. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.…”