Integration of paper-based microfluidic devices with commercial electrochemical readers

Nie, Zhihong; Deiss, Frédérique; Liu, Xinyu; Akbulut, Özge; Whitesides, George M.

doi:10.1039/c0lc00237b

Cited by 461 publications

(376 citation statements)

References 25 publications

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“…Nie et al successfully detected glucose, cholesterol, lactate, and alcohol by integrating an electrode screen-printed on Whatman #1 chromatographic paper in a commercial hand-held glucometer device. They impressively lowered the cost of the device, from 0.5 $/strip to 0.014 $/strip, by simply replacing the classic plastic-based test strips with chromatographic paper [22]. In spite of this relevant finding, office paper might serve as a novel substrate in sensor manufacturing and, along with Whatman #1, it represents a sustainable tool to reduce the use of non-cellulosic substrates, i.e.…”

Section: Introductionmentioning

confidence: 99%

Sustainable monitoring of Zn(II) in biological fluids using office paper

Cinti

Lellis

Moscone

et al. 2017

Sensors and Actuators B: Chemical

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a b s t r a c tHerein, we describe a sustainable and inexpensive approach to monitor Zn(II) in biological fluids by fabricating an office paper-based electrochemical sensor. By following two easy steps, consisting of wax patterning and electrode screen-printing, the office paper provides an effective electroanalytical tool that is easily extensible to a broad range of analytes. This approach would be able to develop affordable userfriendly sensing devices, tackling the lack of resources in regions with poor-settings/facilities. In order to provide more details regarding the screen-printed electrodes fabrication, office paper, Whatman #1 chromatrographic paper, and polyester have been characterized with electrochemical, morphological, and mechanical tests and compared. Using office paper, Zn(II) has been detected linearly up to 2 g/mL with a detection limit equal to 25 ng/mL and a relative standard deviation of 8%. To highlight the feasibility, reliability, and easiness of the proposed electrochemical sensor, Zn(II) has been detected in serum and sweat at physiological level ( g/mL), and the accuracy of the method has been verified by satisfactory recoveries close to 100%.

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Section: Introductionmentioning

confidence: 99%

Sustainable monitoring of Zn(II) in biological fluids using office paper

Cinti

Lellis

Moscone

et al. 2017

Sensors and Actuators B: Chemical

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“…The use of carbon as a base material to construct screen-printing devices have been employed, during the last decades, because it has adequate properties that allows not only its simple use as porous materials, but also it permits surface modification and is cheap [5][6][7][8][9]. Fabrication of paper-based devices has also been developed in the last ten years, but still it is a challenge.…”

Section: Introductionmentioning

confidence: 99%

Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example

Ribau¹,

Fortunato²

2018

JPP

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Abstract:The use of screen-printing biosensors has been updated in this article as a tool to analyze the electron transfer process involving redox proteins or enzymes. The aim of this research was to fabricate a simple apparatus which allowed the use of the enzymes (in the solid state) to maintain their stability. To prove this concept an enzyme in the solid state was mixed with the carbon ink and this mixture was used to print the working electrode. We choose as proving the alcohol dehydrogenase. The first reason is because it metabolizes the alcohol, which can be present in biological samples of blood, saliva and urine and also in the beverage; the second is that this enzyme is still a challenge to electrochemistry due to having lower stability in sensors. The results show that in this device the enzyme was active and stable during all the experiments and in the experimental conditions that could catalyze the ethanol to acetaldehyde. These devices have the advantage of being disposable, cheap and are easy to fabricate. And also, they do not need expensive tools to be fabricated, they only need 2 µL of electrolyte or sample, and they need lower amounts of enzyme to permit electrochemical studies.

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“…[14][15][16][17] With advancement of portable electrochemical instrumentation, the integration of E-lPADs with portable, low-cost electrochemical readers (i.e., potentiostat) makes it possible for paper-based tests to be carried out remotely at the POC and/or in the field with enhanced analytical performance. [18][19][20] Aiming at POC diagnosis of HIV/HCV co-infection, we develop a paper-based, integrated diagnostic platform capable of multiplexed electrochemical enzyme-linked immunosorbent assays (ELISAs) of HIV/HCV antibody markers and remote transmission of diagnostic results for telemedicine. There exists E-lPADs for carrying out ELISAs of cancer and tumor markers.…”

Section: Introductionmentioning

confidence: 99%

A portable paper-based microfluidic platform for multiplexed electrochemical detection of human immunodeficiency virus and hepatitis C virus antibodies in serum

Zhao

Liu

2016

Biomicrofluidics

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This paper presents a portable paper-based microfluidic platform for multiplexed electrochemical detection of antibody markers of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) in serum samples. To our best knowledge, this is the first paper-based electrochemical immunosensing platform, with multiplexing and telemedicine capabilities, for diagnosing HIV/HCV co-infection. The platform consists of an electrochemical microfluidic paper-based immunosensor array (E-lPIA) and a handheld multi-channel potentiostat, and is capable of performing enzyme-linked immunosorbent assays simultaneously on eight samples within 20 min (using a prepared E-lPIA). The multiplexing feature of the platform allows it to produce multiple measurement data for HIV and HCV markers from a single run, and its wireless communication module can transmit the results to a remote site for telemedicine. The unique integration of paper-based microfluidics and mobile instrumentation renders our platform portable, low-cost, user-friendly, and high-throughput. V C 2016 AIP Publishing LLC.

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Integration of paper-based microfluidic devices with commercial electrochemical readers

Cited by 461 publications

References 25 publications

Sustainable monitoring of Zn(II) in biological fluids using office paper

Sustainable monitoring of Zn(II) in biological fluids using office paper

Achieving Enzyme Stability Using a Simple Fabrication Procedure: The Alcohol Dehydrogenase Example

A portable paper-based microfluidic platform for multiplexed electrochemical detection of human immunodeficiency virus and hepatitis C virus antibodies in serum

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