Integrative analyses of transcriptome and metabolome shed light on the regulation of secondary metabolites in pseudobulbs of two Bletilla striata (Thunb.) Reichb.f. varieties

Chen, Jin; Zhu, Feiying; Liu, Ling; Yi, Lixiong; Dai, Yanjiao; Chen, Shan; He, Ai-Guo; Xia, Shitou

doi:10.1016/j.jarmap.2020.100280

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…Based on the above ideas, it is thought that the pseudobulbs could act as a reservoir organ for water, nutrients, and carbohydrates [ 39 , 40 , 41 ] but also, as Zotz [ 40 ] mentions, the importance of the backshoots in providing water, carbohydrates, and nutrients to new growth structures in the next cycle in Dimerandra emarginata (Orchidaceae); the data from our study suggest that the pseudobulb plays an important role in regulating flowering in L. anceps as a reservoir through the accumulation of endogenous hormones, which is supported in part by Chen et al [ 42 ], who establish a correlation between transcriptome and metabolome in pseudobulbs of Bletilla striata (Orchidaceae), shedding light on the synthesis pathways of bioactive substances, including hormones.…”

Section: Discussionsupporting

confidence: 76%

Endogenous Hormone Profile and Sugars Display Differential Distribution in Leaves and Pseudobulbs of Laelia anceps Plants Induced and Non-Induced to Flowering by Exogenous Gibberellic Acid

Tejeda-Sartorius

Soto-Hernández

Miguel-Chávez

et al. 2022

Plants

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A profile of endogenous hormones and sugars in leaves and pseudobulbs of Laelia anceps subsp. anceps (Orchidaceae) plants induced and non-induced to flowering by the effect of different doses of exogenous gibberellic acid (GA3), considering the current and back growth structures (CGS and BGS), were investigated. A factorial experiment with five doses of GA3 and two growth structures was designed. Adult plants with undifferentiated vegetative buds were selected and sprayed with doses of 0, 400, 600, 800, and 1000 mg GA3 L−1. The main results showed a strong interaction between GA3 dose and growth structures, which promoted the highest kinetin (KIN) concentration in CGS. Exogenous GA3 increased endogenous GA3 in leaves and pseudobulbs induced (I-Leaf and I-PSB) and non-induced (NI-Leaf and NI-PSB) to flowering. For sugar concentration, the 400 mg L−1 GA3 dose promotes significant interaction with the CGS in NI-PSB. In general, the hormone profile revealed opposite balances of endogenous hormone concentrations for KIN, zeatin (ZEA), trans-zeatin (T-ZEA), indoleacetic acid (IAA), indole-3-butyric acid (IBA) and GA3, not only for growth structures but also for vegetative organs analyzed, depending on whether the plants were induced or not induced to flowering, with the highest concentration of endogenous hormones in pseudobulbs. Likewise, different sugar concentration balances were observed. These balances of both endogenous hormones and sugars are likely to be involved in the flowering of L. anceps.

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Section: Discussionsupporting

confidence: 76%

Endogenous Hormone Profile and Sugars Display Differential Distribution in Leaves and Pseudobulbs of Laelia anceps Plants Induced and Non-Induced to Flowering by Exogenous Gibberellic Acid

Tejeda-Sartorius

Soto-Hernández

Miguel-Chávez

et al. 2022

Plants

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“…striata . Chen et al [ 39 ] conducted a study on the transcriptome and metabolome of B . striata varieties with different yield variations, finding significant differences in gene expression and metabolite accumulation.…”

Section: Discussionmentioning

confidence: 99%

Combined metabolomics and transcriptomics reveal the secondary metabolite networks in different growth stages of Bletilla striata (Thunb.) Reichb.f.

Chen,

Wang,

et al. 2024

PLoS ONE

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Background Bletilla striata (Thunb.) Reichb.f. (B. striata) is a traditional Chinese medicinal herb. B. striata polysaccharides (BSP), stilbenes and 2-isobutyl malic acid glucosoxy-benzyl ester compounds are the main active ingredients in B. striata. However, there is limited report on the changes of medicinal components and their biosynthesis regulation mechanisms in the tubers of B. striata at different stages. Method The tubers of B. striata were collected during the flowering period, fruiting period, and harvest period to determine the total polysaccharide content using the phenol sulfuric acid method. The changes in secondary metabolites in the tubers at these stages were analyzed by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS), and transcriptomics was conducted for further exploration of their biosynthetic pathways. Result The BSP content gradually increases from the flowering period to the fruiting period as the tubers develop, reaching its peak, but subsequently decreases at harvest time, which may be associated with the germination of B. striata buds in later stage. A total of 294 compounds were identified in this study. Among them, a majority of the compounds, such as 2-isobutyl malate gluconoxy-benzyl ester, exhibited high content during the fruit stage, while stilbenes like coelonin, 3’-O-methylbatatasin III, and blestriarene A accumulated during the harvesting period. The transcriptome data also revealed a substantial number of differentially expressed genes at various stages, providing a partial explanation for the complex changes in metabolites. We observed a correspondence between the expression pattern of GDP-Man biosynthesis-related enzyme genes and cumulative changes in BSP. And identified a positive correlation between 9 transcription factors and genes associated with polysaccharide biosynthesis, while 5 transcription factors were positively correlated with accumulation of 2-isobutyl malate gluconoxy-benzyl ester compounds and 5 transcription factors exhibited negative correlated with stilbene accumulation. Conclusion It is imperative to determine the appropriate harvesting period based on the specific requirements of different active ingredients and the accumulation patterns of their metabolites. Considering the involvement of multiple transcription factors in the biosynthesis and accumulation of its active ingredients, a comprehensive investigation into the specific regulatory mechanisms that facilitate high-quality cultivation of B. striata is imperative.

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“…As a result of its commercial value, some molecular and omics studies for B. striata have been published recently. For example, Chen, Zeng, et al (2021) revealed the regulation of secondary metabolites in pseudobulbs of B. striata through comprehensive analyses of the metabolome and the transcriptome (Chen, Zhu, et al, 2021). Lu et al (2020) found that BsSOD1 is involved in the defense response of B. striata (Lu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…For example, Chen, Zeng, et al. (2021) revealed the regulation of secondary metabolites in pseudobulbs of B. striata through comprehensive analyses of the metabolome and the transcriptome (Chen, Zhu, et al., 2021). Lu et al.…”

Section: Introductionmentioning

confidence: 99%

Haplotype‐resolved genome assembly of Bletilla striata (Thunb.) Reichb.f. to elucidate medicinal value

et al. 2022

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Bletilla striata, commonly known as baiji, is a species used in traditional Chinese medicine; it is highly regarded for its medicinal applications and therefore has high economic value. Here, we report a highquality haplotype-resolved genome of B. striata, haplotype A (2.37 Gb, with a scaffold N50 of 146.39 Mb and a contig N50 of 1.65 Mb) and haplotype B (2.43 Gb, with a scaffold N50 of 150.22 Mb and a contig N50 of 1.66 Mb), assembled from high-fidelity (HiFi) reads and chromosome conformation capture (Hi-C) reads. We find evidence that B. striata has undergone two whole-genome duplication (WGD) events: an ancient WGD event shared by most monocots and a recent WGD event unique to all orchids. We also reconstructed the ancestral orchid karyotype (AOK) of 18 ancient chromosomes and the evolutionary trajectories of 16 modern B. striata chromosomes. Comparative genomic analysis suggests that the expanded gene families of B. striata might play important roles in secondary metabolite biosynthesis and environmental adaptation. By combining genomic and transcriptomic data, we identified the 10 core members from nine gene families that were probably involved in B. striata polysaccharide (BSP) biosynthesis. Based on virus-induced gene silencing (VIGS) and yeast two-hybrid experiments, we present an MYB transcription factor (TF), BsMYB2, that can regulate BSP biosynthesis by directly interacting with eight key BSP-related genes: sacA1, HK1, scrK1, scrK2, GPI1, manA1, GMPP1 and UGP2_1. Our study will enhance the understanding of orchid evolution and accelerate the molecular-assisted breeding of B. striata for improving traits of medicinal value.

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Integrative analyses of transcriptome and metabolome shed light on the regulation of secondary metabolites in pseudobulbs of two Bletilla striata (Thunb.) Reichb.f. varieties

Cited by 8 publications

References 25 publications

Endogenous Hormone Profile and Sugars Display Differential Distribution in Leaves and Pseudobulbs of Laelia anceps Plants Induced and Non-Induced to Flowering by Exogenous Gibberellic Acid

Endogenous Hormone Profile and Sugars Display Differential Distribution in Leaves and Pseudobulbs of Laelia anceps Plants Induced and Non-Induced to Flowering by Exogenous Gibberellic Acid

Combined metabolomics and transcriptomics reveal the secondary metabolite networks in different growth stages of Bletilla striata (Thunb.) Reichb.f.

Haplotype‐resolved genome assembly of Bletilla striata (Thunb.) Reichb.f. to elucidate medicinal value

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