Integrative analyses of transcriptomics and metabolomics in Raphidocelis subcapitata treated with clarithromycin

Jie, Peng; Guo, Jiahua; Yuan, Lei; Mo, Jiezhang; Sun, Haotian; Song, Jinxi

doi:10.1016/j.chemosphere.2020.128933

Cited by 38 publications

(8 citation statements)

References 75 publications

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“…Carotenoids are not only involved in light collection, but also in the protection of the photosynthetic apparatus against ROS-associated damage (Knauert and Knauer, 2008). It has already been observed that in R. subcapitata exposed to low doses of the macrolide clarithromycin, carotenoids synthesis increased, allowing cells to increase their resistance towards this antibiotic (Peng et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…These results demonstrated once more the importance of non-photochemical processes as the main dissipation of energy in exposed microalgae and are in line with what has been seen in other studies. Other macrolides such as clarithromycin, roxithromycin and erythromycin negatively affected not only the xenobiotic metabolism of exposed R. subcapitata, but also their electron transport and energy synthesis (Guo et al, 2020Peng et al, 2021). In a tentative for compensating the loss of energy, photosynthesis increased in exposed microalgae to obtain more energy.…”

Section: Discussionmentioning

confidence: 99%

“…In a tentative for compensating the loss of energy, photosynthesis increased in exposed microalgae to obtain more energy. However, the over-excitation of light-collecting antenna and light intensity can also lead to an increment in ROS formation, instigating genotoxicity and DNA damage (Peng et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Specific toxicity of azithromycin to the freshwater microalga Raphidocelis subcapitata

Almeida

Gomes

Lomba

et al. 2021

Ecotoxicology and Environmental Safety

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Pharmaceuticals are produced to inflict a specific physiological response in organisms. However, as only partially metabolized after administration, these types of compounds can also originate harmful side effects to non-target organisms. Additionally, there is still a lack of knowledge on the toxicological effects of legacy pharmaceuticals such as the antibiotic azithromycin. This macrolide occurs at high concentrations in the aquatic environment and can constitute a threat to aquatic organisms that are at the basis of the aquatic food chain, namely microalgae. This study established a high-throughput methodology to study the toxicity of azithromycin to the freshwater microalga Raphidocelis subcapitata. Flow cytometry and pulse amplitude modulated (PAM) fluorometry were used as screening tools. General toxicity was shown by effects in growth rate, cell size, cell complexity, cell viability and cell cycle. More specific outcomes were indicated by the analysis of mitochondrial and cytoplasmatic membrane potentials, DNA content, formation of ROS and LPO, natural pigments content and photosystem II performance. The specific mode of action (MoA) of azithromycin to crucial components of microalgae cells was revealed. Azithromycin had a negative impact on the regulation of energy dissipation at the PSII centers, along with an insufficient protection by the regulatory mechanisms leading to photodamage. The blockage of photosynthetic electrons led to ROS formation and consequent oxidative damage, affecting membranes and DNA. Overall, the used methodology exhibited its high potential for detecting the toxic MoA of compounds in microalgae and should be considered for future risk assessment of pharmaceuticals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Specific toxicity of azithromycin to the freshwater microalga Raphidocelis subcapitata

Almeida

Gomes

Lomba

et al. 2021

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

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“…For example, metabolomics has been used to identify correlations between the root microbiome and plant gene translocation in varying plant functions when perturbed by mine drainage pollution that ultimately improved its adaptability and phytoremediation potential [ 53 ]. Integrative analyses of transcriptomes and metabolomes in microalgae ( Raphidocelis subcapitata ) treated with the antibiotic clarithromycin [ 90 ] identified impacts to biosynthesis and photosynthesis, highlighting the inhibitory effects of macrolide antibiotics.…”

Section: Environmental Monitoringmentioning

confidence: 99%

Omics-based ecosurveillance for the assessment of ecosystem function, health, and resilience

Beale

Jones

Bose

et al. 2022

Emerging Topics in Life Sciences

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Current environmental monitoring efforts often focus on known, regulated contaminants ignoring the potential effects of unmeasured compounds and/or environmental factors. These specific, targeted approaches lack broader environmental information and understanding, hindering effective environmental management and policy. Switching to comprehensive, untargeted monitoring of contaminants, organism health, and environmental factors, such as nutrients, temperature, and pH, would provide more effective monitoring with a likely concomitant increase in environmental health. However, even this method would not capture subtle biochemical changes in organisms induced by chronic toxicant exposure. Ecosurveillance is the systematic collection, analysis, and interpretation of ecosystem health-related data that can address this knowledge gap and provide much-needed additional lines of evidence to environmental monitoring programs. Its use would therefore be of great benefit to environmental management and assessment. Unfortunately, the science of ‘ecosurveillance’, especially omics-based ecosurveillance is not well known. Here, we give an overview of this emerging area and show how it has been beneficially applied in a range of systems. We anticipate this review to be a starting point for further efforts to improve environmental monitoring via the integration of comprehensive chemical assessments and molecular biology-based approaches. Bringing multiple levels of omics technology-based assessment together into a systems-wide ecosurveillance approach will bring a greater understanding of the environment, particularly the microbial communities upon which we ultimately rely to remediate perturbed ecosystems.

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“…Details in the sample cleanup procedures and instrumental settings can be found in Supporting Information (Tables S1 and S2). Since the variation between the nominal and measured concentrations was <20%, the nominal concentration was used for further investigation (OECD 2011;Guo et al 2020;Peng et al 2020). In addition, the photodecomposition of ERY was investigated.…”

Section: Antibiotic Analysismentioning

confidence: 99%

Growth Inhibition and Oxidative Stress in Two Green Algal Species Exposed to Erythromycin

Yang

Ren

et al. 2021

J American Water Resour Assoc

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Chlorella vulgaris (C. vulgaris) and Raphidocelis subcapitata (R. subcapitata) are two model algal species applied for the testing of chemicals, whereas a considerable variation in their sensitivity to antibiotics has been reported. The difference in species sensitivity may be attributed to the xenobiotic‐induced oxidative stress in algal cells. Thus, this study compared the responses of these two model green algae toward the exposure of erythromycin (ERY), a macrolide antibiotic, at the concentration levels of 0, 20, 40, 60 μg/L, and elucidated the toxic mechanism of action from the point of oxidative damage and antioxidant defense. To achieve this, effects on the endpoints of algal growth, malondialdehyde (MDA), antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GP], glutathione, and glutathione S‐transferase), and photosynthetic pigment contents were evaluated. After exposing for seven days, the growth of R. subcapitata was significantly stimulated at ERY 20 μg/L but inhibited at the higher exposure level of 60 μg/L. Contrarily, hormesis in C. vulgaris exposed to all ERY treatments was detected. With the increase in ERY exposure, MDA content, and the antioxidant activity of SOD, CAT, GP in R. subcapitata were gradually enhanced. In C. vulgaris, a declining trend in the MDA content and the activity of SOD, CAT, and GP was detected. These results provide insights into the ERY‐altered production of reactive oxygen species and the antioxidant responses in two algae.

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Integrative analyses of transcriptomics and metabolomics in Raphidocelis subcapitata treated with clarithromycin

Cited by 38 publications

References 75 publications

Specific toxicity of azithromycin to the freshwater microalga Raphidocelis subcapitata

Specific toxicity of azithromycin to the freshwater microalga Raphidocelis subcapitata

Omics-based ecosurveillance for the assessment of ecosystem function, health, and resilience

Growth Inhibition and Oxidative Stress in Two Green Algal Species Exposed to Erythromycin

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