Integrative computational mRNA–miRNA interaction analyses of the autoimmune-deregulated miRNAs and well-known Th17 differentiation regulators: An attempt to discover new potential miRNAs involved in Th17 differentiation

Honardoost, Mohammad Amin; Naghavian, Reza; Ahmadinejad, Fereshteh; Hosseini, Aref; Ghaedi, Kamran

doi:10.1016/j.gene.2015.08.043

Cited by 38 publications

(34 citation statements)

References 59 publications

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“…Dysregulated miRNAs have been announced to pertinent to multitudinous ailments [28,29]. Previous study corroborated that miRNAs play critical roles in various ailments which are linked to oxidative damage [30].…”

Section: Discussionmentioning

confidence: 87%

Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a

Liu

Fan

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Spinal cord injury (SCI) can lead to varying degrees of sensory and motor dysfunction. Salvianolic acid B (Sal-B) is the dominating bioactive constituent of Danshen, which has been reported to alleviate liver fibrosis and exert neuroprotective effects. But, the influence of Sal-B in SCI remains mysterious. The research planned to delve the protective function of Sal-B in hydrogen peroxide (H 2 O 2)-caused PC-12 cell injury. H 2 O 2-caused PC-12 cells injury model was built, CCK-8, Transwell and flow cytometry experiments were enforced to assess cell proliferation, migration and apoptosis. The microRNA (miR)-26a plasmid and the matching control were transfected into PC-12 cells, subsequently, the influence of miR-26a inhibition in H 2 O 2-corrupted PC-12 cells was evaluated. The cell growth-correlated factors and PI3K/AKT and MEK/ERK pathways were assayed through western blot assay. Results corroborated that Sal-B eased H 2 O 2-evoked injury in PC-12 cells. Ascended miR-26a was monitored in Sal-B and H 2 O 2exposed cells. MiR-26a inhibition annulled the protective action of Sal-B in H 2 O 2-corrupted cells. The protective function of Sal-B was enabled through activating PI3K/AKT and MEK/ERK pathways. These findings delineated that Sal-B protected PC-12 cells against H 2 O 2-caused injury through ascending miR-26a via initiating PI3K/AKT and MEK/ERK pathways. HIGHLIGHTS H 2 O 2 causes PC-12 cell injury; Sal-B eases H 2 O 2-caused PC-12 cell injury; Sal-B protects PC-12 cells against H 2 O 2-caused injury via elevating miR-26a; Sal-B activates AKT and MEK/ERK pathways via modulating miR-26a.

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Section: Discussionmentioning

confidence: 87%

Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a

Liu

Fan

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Upregulation of miR-15a-5p in IBD patient is reported in [45]. By sponging miR-1207-5p a long noncoding RNA BC032469 upregulates hTERT expression which promotes proliferation in gastric cancer [46].…”

Section: Relevance Of the Top Mirnas To Ibdmentioning

confidence: 99%

Discovery of inflammatory bowel disease-associated miRNAs using a novel bipartite clustering approach

Altaf‐Ul‐Amin

Karim

et al. 2020

BMC Med Genomics

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Background: Multidimensional data mining from an integrated environment of different data sources is frequently performed in computational system biology. The molecular mechanism from the analysis of a complex network of gene-miRNA can aid to diagnosis and treatment of associated diseases. Methods: In this work, we mainly focus on finding inflammatory bowel disease (IBD) associated microRNAs (miRNAs) by biclustering the miRNA-target interactions aided by known IBD risk genes and their associated miRNAs collected from several sources. We rank different miRNAs by attributing to the dataset size and connectivity of IBD associated genes in the miRNA regulatory modules from biclusters. We search the association of some top-ranking miRNAs to IBD related diseases. We also search the network of discovered miRNAs to different diseases and evaluate the similarity of those diseases to IBD. Results: According to different literature, our results show the significance of top-ranking miRNA to IBD or related diseases. The ratio analysis supports our ranking method where the top 20 miRNA has approximately tenfold attachment to IBD genes. From disease-associated miRNA network analysis we found that 71% of different diseases attached to those miRNAs show more than 0.75 similarity scores to IBD. Conclusion: We successfully identify some miRNAs related to IBD where the scoring formula and disease-associated network analysis show the significance of our method. This method can be a promising approach for isolating miRNAs for similar types of diseases.

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“…However, the effect of miR-340 on IL-17A has never been assessed under a condition that induces Th17 differentiation. In addition, it has been reported before that miR-340 could suppress Th17 response based on computational analysis, although the mechanism is through the direct targeting of the positive regulators of Th17 differentiation (24).…”

Section: Discussionmentioning

confidence: 99%

“…miRNAs mediate the autoimmune disease pathologic condition at least in part through the regulation of Th17 response. However, among the few miRNAs identified so far that play possible roles in the differentiation and pathogenesis of Th17 cells, they all regulate the Th17 response through indirect targeting of negative or positive regulators of Th17 differentiation (24). For example, miR-326 could promote Th17 differentiation through targeting Ets-1, a negative regulator of Th17 differentiation (25).…”

mentioning

confidence: 99%

miR-340 Alleviates Psoriasis in Mice through Direct Targeting of IL-17A

Bian

Liu

Fan

et al. 2018

The Journal of Immunology

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Th17 cell is a well-known lineage of CD4 effector Th cells that selectively produce IL-17A and play critical roles during the pathogenesis of autoimmune disease. A microRNA (miRNA) is a small noncoding RNA molecule that functions in posttranscriptional regulation of gene expression. Recently, an increasing number of studies have demonstrated that multiple miRNAs are dysregulated in patients with various autoimmune diseases and mediate autoimmune disease pathologic condition at least in part through the regulation of Th17 response. However, among the few miRNAs identified so far that play possible roles in the differentiation of Th17 cells, they all regulate the Th17 response through targeting negative or positive regulators of Th17 differentiation. In the current study, we sought to identify new miRNAs that can directly regulate the expression of IL-17A, the most important cytokine produced by Th17 cells. Our results showed that the 3' untranslated region of mouse IL-17A can act as a negative regulatory element to downregulate gene expression. Further study revealed that miR-340 can specifically bind to the 3' untranslated region of mouse IL-17A and downregulate the expression of endogenous IL-17A. More importantly, we demonstrated that treatment with miR-340 alleviates the clinical severity of imiquimod-induced psoriasis in mice through the downregulation of IL-17A. These data indicate that miR-340 may be a useful therapeutic target for the treatment of psoriasis and other IL-17A-mediated autoimmune diseases.

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Integrative computational mRNA–miRNA interaction analyses of the autoimmune-deregulated miRNAs and well-known Th17 differentiation regulators: An attempt to discover new potential miRNAs involved in Th17 differentiation

Cited by 38 publications

References 59 publications

Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a

Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a

Discovery of inflammatory bowel disease-associated miRNAs using a novel bipartite clustering approach

miR-340 Alleviates Psoriasis in Mice through Direct Targeting of IL-17A

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