Integrative Gene Cloning and Expression System for <i>Streptomyces</i> sp. US 24 and <i>Streptomyces</i> sp. TN 58 Bioactive Molecule Producing Strains

Sioud, Samiha; Aigle, Bertrand; Karray-Rebai, Ines; Smaoui, Slim; Béjar, Samír; Mellouli, Lotfi

doi:10.1155/2009/464986

Cited by 10 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, after conjugation, tandems of engineered plasmids carrying the serine recombinase-based recombination system of the Streptomyces phage φC31 are frequently observed. They probably result from successive and independent site-specific recombinations, that is, site-specific accretion, but may also result from a site-specific integration and a subsequent homologous recombination of the integrated copy with a free copy of the plasmid (Combes et al, 2002;Eustaquio et al, 2005;Sioud et al, 2009).…”

Section: Accretion Catalyzed By a Serine Recombinasementioning

confidence: 99%

Conjugative and mobilizable genomic islands in bacteria: evolution and diversity

et al. 2014

View full text Add to dashboard Cite

Horizontal transfer of genomic islands (GEIs), that is, chromosomal regions encoding functions that can be advantageous for the host, plays a key role in bacterial evolution, but their mechanisms of transfer remained elusive for a long time. Recent data suggest that numerous GEIs belong to noncanonical classes of mobile genetic elements (MGEs) that can transfer by conjugation. Among them, the integrative and conjugative elements encode their own excision, conjugative transfer, and integration, whereas the integrative mobilizable elements are autonomous for excision and integration but require the conjugation machinery of helper elements to transfer. Others can self-transfer but require the recombination machinery of the recipient cell to integrate. All these MGEs evolve by acquisition, deletion, or exchange of modules, that is, groups of genes involved in the same function. Moreover, composite GEIs can result from the insertion of a MGE within another or from the site-specific integration of an incoming MGE into one of the recombination sites flanking a resident GEI (tandem accretion). Tandem accretion enables the cis-conjugative mobilization of highly degenerated and nonautonomous GEIs, the cis-mobilizable elements. All these mechanisms contribute to the plasticity and complex evolution of GEIs and explain the highly diverse tableau revealed by more and more genome comparisons.

show abstract

Section: Accretion Catalyzed By a Serine Recombinasementioning

confidence: 99%

Conjugative and mobilizable genomic islands in bacteria: evolution and diversity

et al. 2014

View full text Add to dashboard Cite

show abstract

“… 32 The resulting pMarG-34S was introduced into S. venezuelae strain by conjugation following the established protocol, except mannitol-soy flour agar was replaced with AS1 media containing 10 mM MgCl 2 . 33 Thiostrepton-resistant exconjugants representing S. venezuelae strains host with marG overexpression plasmids. The new strain was named S. venezuelae MarG.…”

Section: Experimental Sectionmentioning

confidence: 99%

Stereospecific Synthesis of 23-Hydroxyundecylprodiginines and Analogues and Conversion to Antimalarial Premarineosins via a Rieske Oxygenase Catalyzed Bicyclization

Kancharla

Salem

et al. 2014

J. Org. Chem.

View full text Add to dashboard Cite

Facile and highly efficient synthetic routes for the synthesis of (S)- and (R)-23-hydroxyundecylprodiginines ((23S)-2, and (23R)-2), 23-ketoundecylprodiginine (3), and deuterium-labeled 23-hydroxyundecylprodiginine ([23-d]-2) have been developed. We demonstrated a novel Rieske oxygenase MarG catalyzed stereoselective bicyclization of (23S)-2 to premarineosin A (4), a key step in the tailoring process of the biosynthesis of marineosins, using a marG heterologous expression system. The synthesis of various A–C-ring functionalized prodiginines 32–41 was achieved to investigate the substrate promiscuity of MarG. The two analogues 32 and 33 exhibit antimalarial and cytotoxic activities stronger than those of the marineosin intermediate 2, against Plasmodium falciparum strains (CQS-D6, CQR-Dd2, and 7G8) and hepatocellular HepG2 cancer cell line, respectively. Feeding of 34–36 to Streptomyces venezuelae expressing marG led to production of novel premarineosins, paving a way for the production of marineosin analogues via a combinatorial synthetic/biosynthetic approach. This study presents the first example of oxidative bicyclization mediated by a Rieske oxygenase.

show abstract

“…To investigate the effects of pimE overexpression on natamycin production and kinetics of cell growth, S. gilvosporeus 712, S. gilvosporeus-pSET152, and S. gilvosporeus swjs-801 were cultured in 250 ml shake flasks of a 30 ml working volume for 120 h. S. gilvosporeus-pSET152, loaded with null plasmid, did not display any significant difference in cell growth and natamycin production compared with the control strain, which presented similarly as Streptomyces sp. US 24 and S. gilvosporeus-vgb [22,24] (data not shown). Cell growth (Fig.…”

Section: Effects Of Pime Overexpression On Natamycin Production In Shmentioning

confidence: 85%

Improvement of Natamycin Production by Cholesterol Oxidase Overexpression in Streptomyces gilvosporeus

Wang¹,

Wang²,

Zong³

et al. 2016

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

Natamycin is a widely used antifungal antibiotic. For natamycin biosynthesis, the gene pimE encodes cholesterol oxidase, which acts as a signalling protein. To confirm the positive effect of the gene pimE on natamycin biosynthesis, an additional copy of the gene pimE was inserted into the genome of Streptomyces gilvosporeus 712 under the control of the ermE* promoter (permE*) using intergeneric conjugation. Overexpression of the target protein engendered 72% and 81% increases in the natamycin production and cell productivity, respectively, compared with the control strain. Further improvement in the antibiotic production was achieved in a 1 L fermenter to 7.0 g/l, which was a 153% improvement after 120 h cultivation. Exconjugants highly expressing pimE and pimM were constructed to investigate the effects of both genes on the increase of natamycin production. However, the co-effect of pimE and pimM did not enhance the antibiotic production obviously, compared with the exconjugants highly expressing pimE only. These results suggest not only a new application of cholesterol oxidase but also a useful strategy to genetically engineer natamycin production.

show abstract

Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

Cited by 10 publications

References 53 publications

Conjugative and mobilizable genomic islands in bacteria: evolution and diversity

Conjugative and mobilizable genomic islands in bacteria: evolution and diversity

Stereospecific Synthesis of 23-Hydroxyundecylprodiginines and Analogues and Conversion to Antimalarial Premarineosins via a Rieske Oxygenase Catalyzed Bicyclization

Improvement of Natamycin Production by Cholesterol Oxidase Overexpression in Streptomyces gilvosporeus

Contact Info

Product

Resources

About