Integrative metabolic and microbial profiling on patients with Spleen-yang-deficiency syndrome

Zhou, Lin; Wu, Ye; Zu, Xianpeng; Xie, Haisheng; Li, Houkai; Li, Yiping; Zhang, Weidong

doi:10.1038/s41598-018-24130-7

Cited by 78 publications

(59 citation statements)

References 45 publications

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“…Significant correlations between soil properties and the fungal communities at OTU level were determined via Spearman's correlations using >0.8 or <−0.8 as the threshold, with a cutoff of P <0.01 (Faust et al, 2012). All the data analysis for correlations was finished in online 'i-sanger' (http://www.i-sanger.com/) developed by Majorbio Bio-Pharm Technology Co. Ltd (Lin et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping

Zhu

Ji³

et al. 2020

PeerJ

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Rhizospheric and endophytic fungi are key factors which influence plant fitness and soil fertility. Atractylodes macrocephala is one of the best-known perennial herbs used in traditional Chinese medicine. Continuous cropping has been shown to have a negative effect on its growth and renders it more susceptible to microbial pathogen attacks. In this study, we investigated the effects of continuous cropping on the endophytic and rhizospheric fungi associated with A. macrocephala using culture-independent Illumina MiSeq. Continuous cropping was found to decrease fungal diversity inside plant roots, stems, leaves and tubers. Additionally, we found that the structure and diversity of rhizospheric and endophytic fungal communities were altered by root-rot disease. Fusarium was overrepresented among root-rot rhizospheric and endophytic fungi, indicating that it has a major negative impact on plant health during A. macrocephala monocropping. Canonical correspondence analysis of the control and diseased samples revealed that pH, hydrolysis N, electrical conductivity and Hg content were well-correlated with fungal community composition during continuous cropping. Taken together, these results highlight the ecological significance of fungal communities in maintaining plant fitness and will guide the development strategies to attenuate the negative impacts of A. macrocephala continuous cropping.

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Section: Discussionmentioning

confidence: 99%

Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping

Zhu

Ji³

et al. 2020

PeerJ

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“…Furthermore, the relative abundance of f-Lachnospiraceae was decreased by HFD, but increased by AFG. f-Lachnospiraceae has been reported to be associated with anti-inflammatory activity [18], host mucosal integrity [19], the consumption of energy and the level of leptin [20]. Fructan [21,22] and whole garlic [23] have been reported to increase the abundance of f-Lachnospiraceae, but alliin was found to decreased it [24].…”

Section: Discussionmentioning

confidence: 99%

Modulation of Allicin-Free Garlic on Gut Microbiome

Chen

Nakasone²,

Xie

et al. 2020

Molecules

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The allicin diallyldisulfid-S-oxide, a major garlic organosulfur compound (OSC) in crushed garlic (Allium sativum L.), possesses antibacterial effects, and influences gut bacteria. In this study, we made allicin-free garlic (AFG) extract and investigated its effects on gut microbiome. C57BL/6N male mice were randomly divided into 6 groups and fed normal diet (ND) and high-fat diet (HFD) supplemented with or without AFG in concentrations of 1% and 5% for 11 weeks. The genomic DNAs of feces were used to identify the gut microbiome by sequencing 16S rRNA genes. The results revealed that the ratio of p-Firmicutes to p-Bacteroidetes increased by aging and HFD was reduced by AFG. In particular, the f-Lachnospiraceae, g-Akkermansia, and g-Lactobacillus decreased by aging and HFD was enhanced by AFG. The g-Dorea increased by aging and HFD decreased by AFG. In addition, the ratio of glutamic-pyruvic transaminase to glutamic-oxaloacetic transaminase (GPT/GOT) in serum was significantly increased in the HFD group and decreased by AFG. In summary, our data demonstrated that dietary intervention with AFG is a potential way to balance the gut microbiome disturbed by a high-fat diet.

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“…The gut microbiota is an indispensable "metabolic organ" that participates in nutrient processing and the production of essential compounds, such as short-chain fatty acids and bile acids, and it contributes to gastrointestinal system maturation and immune system shaping (Lin et al, 2018). Numerous endogenous and exogenous factors affect microbial composition, such as the host's physiology, immunity, diet, antibiotics and environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver–Gut Axis

Liu

et al. 2020

Front. Pharmacol.

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Antibiotics-induced changes in intestinal flora (dysbiosis) may have various effects on the host. Dysbiosis is associated with numerous metabolites including bile acids, which are produced in the liver from cholesterol and metabolized in the gut by intestinal microbiota. Total phenolic extracts of Citrus aurantium L. (TPE-CA) are rich in dietary flavanones and their glycosyl derivatives, including flavones, flavonols, polymethoxyflavones and coumarins, which exert positive health effects on the microbiota. The aim of this study is to elucidate the interplays between the intestinal microbiota and bile acids metabolism attributed to antibiotics. Mice were exposed to broad-spectrum antibiotics, such as ampicillin, streptomycin and clindamycin, for 14 days. This exposure resulted in reduced bacterial diversity and richness, and destroyed intestinal permeability. The homeostasis of bile acids was also affected. Subsequent TPE-CA administration, counteracted most of the dysbiosis, and reshaped intestinal permeability, these effects occurred via upregulation of zonula occludens 1 and occludin associated proteins and downregulation of serum endotoxin compared to the antibiotics group. TPE-CA maintained the homeostasis of bile acids via modulation of the liver-gut axis related farnesoid X receptor (FXR)/fibroblast growth factor 15 (FGF15) pathway and FXR-targeted protein. Our findings indicated that TPE-CA exerted a protective effect on the restoration of intestinal microbiota composition, reshaped barrier integrity and maintained bile acid homeostasis via the liver-gut axis with antibiotics-induced dysbiosis.

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Integrative metabolic and microbial profiling on patients with Spleen-yang-deficiency syndrome

Cited by 78 publications

References 45 publications

Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping

Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping

Modulation of Allicin-Free Garlic on Gut Microbiome

Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver–Gut Axis

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