Integrative Seed and Leaf Treatment with Ascorbic Acid Extends the Planting Period by Improving Tolerance to Late Sowing Influences in Parsley

Abstract

Abnormal production of reactive oxygen species (ROS) is an undesirable event which occurs in plants due to stress. To meet this event, plants synthesize ROS-neutralizing compounds, including the non-enzymatic oxidant scavenger known as vitamin C: ascorbic acid (AsA). In addition to scavenging ROS, AsA modulates many vital functions in stressed or non-stressed plants. Thus, two-season (2018/2019 and 2019/2020) trials were conducted to study the effect of integrative treatment (seed soaking + foliar spray) using 1.0 or 2.0 mM AsA vs. distilled water (control) on the growth, seed yield, and oil yield of parsley plants under three sowing dates (SDs; November, December, and January, which represent adverse conditions of late sowing) vs. October as the optimal SD (control). The ion balance, osmotic-modifying compounds, and different antioxidants were also studied. The experimental layout was a split plot in a completely randomized block design. Late sowing (December and January) noticeably reduced growth traits, seed and oil yield components, and chlorophyll and nutrient contents. However, soluble sugar, proline, and AsA contents were significantly increased along with the activities of catalase (CAT) and superoxide dismutase (SOD). Under late sowing conditions, the use of AsA significantly increased growth, different yields, essential oil fractions, CAT and SOD activities, and contents of chlorophylls, nutrients, soluble sugars, free proline, and AsA. The interaction treatments of SDs and AsA concentrations indicated that AsA at a concentration of 2 mM was more efficient in conferring greater tolerance to adverse conditions of late sowing in parsley plants. Therefore, this study recommends 2.0 mM AsA for integrative (seed soaking + foliar spraying) treatment to prolong the sowing period of parsley seeds (from October up to December) and avoid damage caused by adverse conditions of late sowing.

Rebalancing Nutrients, Reinforcing Antioxidant and Osmoregulatory Capacity, and Improving Yield Quality in Drought-Stressed Phaseolus vulgaris by Foliar Application of a Bee-Honey Solution

“…The major vital components of the BHS have previously been used individually as foliar applications with success supporting plant performance and increasing its resistance to stress, e.g., organic acids [ 63 ], soluble sugars [ 64 , 65 ], amino acids [ 66 , 67 , 68 ], proline [ 66 , 69 ], essential nutrients [ 70 , 71 , 72 ], ascorbate [ 73 ], B-group vitamins [ 74 , 75 ], selenium [ 7 ], and iodine [ 76 ]. In this paper, all of these components are combined into a solution (BHS) in organic forms, giving this solution a multi-mechanism for plants to adjust to stress conditions and resist stress via signaling pathways and efficient mechanisms.…”

Effect of Spraying with Zinc and Ascorbic Acid on some Antioxidants of Maize (Zea mays L.) Affected by Heat Shock

“…The results showed that spraying C increased the activity of the enzyme with increasing spraying concentrations on the vegetative part of corn. The spraying concentrations 100 and 200 mg.l -1 gave an average of 48.91 and 51.74 U. min.g -1 fw sequentially compared to the control treatment, which gave the lowest average 43.80 U.min.g -1 fw, and this is due to the role, of vitamin C as an important non-enzymatic antioxidant in stimulating the effectiveness of antioxidant enzymes, including the peroxidase enzyme [43] especially with a significant correlation relationship in the increase in peroxidase activity with an increase in the presence of C ‫؛‬ because the latter is a cofactor for oxidation enzymes oxygenase and hydrogenase enzymes, and peroxidase enzyme is a part of which [44] these results agree with [23]. The statistical data in table (4) also showed that the two levels of exposing the seedlings to heat had a significant effect on increasing the activity and activity of the peroxidase enzyme.…”

MORPHO-PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES OF H2S – POLLUTED LETTUCE (Lactuca Sativa L.) PLANTS TO ASCORBIC ACID

“…Ascorbic acid treatment leads to an increase in photosynthetic pigments, which can be attributed to its function in photosynthesis as a substitute electron donor for PSII in abiotic stress conditions. It also protects the photosynthetic apparatus in chloroplasts by reducing ROS activity (Venkatesh and Park, 2014), improving absorption of essential elements, particularly iron, magnesium, potassium, nitrogen, and other cations (Al-Taweel et al, 2022), which are essential for the activation of enzymes and the formation of both chloroplasts and chlorophyll. Furthermore, ascorbic acid is essential for scavenging harmful ROS produced as byproducts of photosynthesis and is a fundamental element of excess photonic energy dissipation mechanisms like the xanthophyll cycle (Yabuta et al, 2007) and the water-water cycle (Neubauer and Yamamoto, 1992;Asada, 2006).…”

“…These results are consistent with those of Elkelish et al (2020), who reported that exogenous ascorbic acid administration can enhance the endogenous ascorbic acid concentration under various stress conditions, hence increasing plant tolerance to a variety of stresses. Al-Taweel et al (2022) discovered that applying 2.0 mM ascorbic acid for integrative (seed soaking + foliar spraying) significantly enhanced the contents of nutrients, soluble sugars, free proline, and ascorbic acid, as well as growth, yields, and essential oil fractions in parsley plants. It was also more successful in giving parsley plants a higher degree of resistance to unfavorable circumstances.…”

“…Plant growth, transpiration, photosynthesis, oxidative defense capacity, photosynthetic pigments, and element nutrition uptake are all stimulated by ascorbic acid (Naz et al, 2016;Al-Taweel et al, 2022). However, the studies concerning the ability of ascorbic acid to minimize or alleviate the negative and harmful effects of H 2 S on plants are scarce.…”