Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents

Abstract

Background Pineapple (Ananas comosus L. Merr.) is the third most important tropical fruit in China. In other crops, farmers can easily judge the nutritional requirements from leaf color. However, concerning pineapple, it is difficult due to the variation in leaf color of the cultivated pineapple varieties. A detailed understanding of the mechanisms of nutrient transport, accumulation, and assimilation was targeted in this study. We explored the D-leaf nitrogen (N), phosphorus (P), and potassium (K) contents, transcriptome, and metabolome of seven pineapple varieties. Results Significantly higher N, P, and K% contents were observed in Bali, Caine, and Golden pineapple. The transcriptome sequencing of 21 libraries resulted in the identification of 14,310 differentially expressed genes in the D-leaves of seven pineapple varieties. Genes associated with N transport and assimilation in D-leaves of pineapple was possibly regulated by nitrate and ammonium transporters, and glutamate dehydrogenases play roles in N assimilation in arginine biosynthesis pathways. Photosynthesis and photosynthesis-antenna proteins pathways were also significantly regulated between the studied genotypes. Phosphate transporters and mitochondrial phosphate transporters were differentially regulated regarding inorganic P transport. WRKY, MYB, and bHLH transcription factors were possibly regulating the phosphate transporters. The observed varying contents of K% in the D-leaves was associated to the regulation of K+ transporters and channels under the influence of Ca2+ signaling. The UPLC-MS/MS analysis detected 873 metabolites which were mainly classified as flavonoids, lipids, and phenolic acids. Conclusions These findings provide a detailed insight into the N, P, K% contents in pineapple D-leaf and their transcriptomic and metabolomic signatures.

Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (Ananas comosus (L.) Merr.) Fruit

“…However, the tested pineapple species did not significantly differ in N, K, or Na content. This result also coincides with the finding of Chen, Zeng, and Zhang [ 3 ]. Shu et al [ 17 ] observed both translucent and non-translucent pineapple pulp have similar total sugar content, but the translucent pulp has higher apoplastic sugar content.…”

“…The HPLC had an analysis column (Fast Carbo-hydrate, 100 × 7.8 mm, Bio-Rad Laboratories, Hercules, Calif). N, P, and K contents were measured using the method described by Chen, Zeng, and Zhang [ 3 ] and were expressed as percent content.…”

“…It is one of the major crops in tropical and subtropical countries [ 1 ] and the third most important tropical fruit in China, utilizing 70,000 hectares of land [ 2 ]. In 2019, China ranked 5th in pineapple production, with an annual harvest of 1727 thousand metric tons [ 3 ]. Pineapple translucency (also known as water core) has been a recurring problem in marketing of the fruits [ 4 ].…”

Potential of Phosphorus Solubilizing Purple Nonsulfur Bacteria Isolated from Acid Sulfate Soil in Improving Soil Property, Nutrient Uptake, and Yield of Pineapple (Ananas comosus L. Merrill) under Acidic Stress

“…Moreover, high acidity and toxicity inhibited plant growth changed the structure and diversity of microorganism [2,[10][11][12][13][14]. P plays a vital role in pineapple growth; this means that P is defcient, and plant growth and quality are afected adversely [15]. However, the isolated and selected bacteria strains should possess properties as biofertilizers and bioremediators.…”

Yield gap reduction of pineapple (Ananas comosus L.) by site-specific nutrient management

“…Correlation between pineapple yield and soil properties: As claimed by Cunha et al [ 29 ] and Chen et al [ 49 ], concentrations of N, P, K, Ca, and Mg nutrients are undeniably crucial for plants to grow and develop. Fig.…”