Integrin trafficking regulates adhesion to fibronectin during differentiation of mouse peri-implantation blastocysts

Schultz, J F; Mayernik, Linda; Rout, Ujjwal K.; Armant, D. Randall

doi:10.1002/(sici)1520-6408(1997)21:1<31::aid-dvg4>3.0.co;2-9

Cited by 65 publications

(45 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously reported [1,2], FBA is developmentally regulated during serum-free culture of blastocysts. The normal developmental profile is distinguished by low levels of FBA after 48 h, high levels at 72 h, and low levels again after 96 h of culture [2].…”

Section: Cannabinoids Promoted Trophoblast Differentiationsupporting

confidence: 73%

“…The normal developmental profile is distinguished by low levels of FBA after 48 h, high levels at 72 h, and low levels again after 96 h of culture [2]. Accordingly, we observed that fluorescent microspheres coated with FN-120 bound poorly to blastocysts cultured for 48 h (Fig.…”

Section: Cannabinoids Promoted Trophoblast Differentiationmentioning

confidence: 53%

“…Before invading the endometrial stroma, mouse primary trophoblast cells differentiate to an adhesive stage, which is characterized by increased fibronectin-binding activity (FBA), expression of ␣ 5 ␤ 1 integrin on the apical surface, and initiation of trophoblast outgrowth on fibronectin [1,2]. This phenotypic transformation of the trophoblast cells is influenced by the maternal environment, which provides biologically active factors that regulate embryonic differentiation and uterine receptivity for implantation [3].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stage-Specific Excitation of Cannabinoid Receptor Exhibits Differential Effects on Mouse Embryonic Development1

Wang

Paria

Dey

et al. 1999

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

Anandamide (N-arachidonoylethanolamine), an arachidonic acid derivative, is an endogenous ligand for both the brain-type (CB1-R) and spleen-type (CB2-R) cannabinoid receptors. We have previously demonstrated that preimplantation mouse embryos express mRNA for these receptors and that the periimplantation uterus contains the highest level of anandamide yet discovered in a mammalian tissue. We further demonstrated that 2-cell mouse embryos exposed to low levels of anandamide (7 nM) or other known cannabinoid agonists in culture exhibit markedly compromised embryonic development to blastocysts and that this effect is mediated by CB1-R. In contrast, the present study demonstrates that blastocysts exposed in culture to the same low levels of cannabinoid agonists exhibited accelerated trophoblast differentiation with respect to fibronectin-binding activity and trophoblast outgrowth. Again, these effects resulted from activation of embryonic CB1-R. There was a differential concentration-dependent effect of cannabinoids on the trophoblast, with an observed inhibition of differentiation at higher doses. These results provide evidence for the first time that cannabinoid effects are differentially executed depending on the embryonic stage and cannabinoid levels in the environment. Since uterine anandamide levels are lowest at the sites of implantation and highest at the interimplantation sites, the new findings imply that site-specific levels of anandamide and/or other endogenous ligands in the uterus may regulate implantation spatially by promoting trophoblast differentiation at the sites of blastocyst implantation.

show abstract

Section: Cannabinoids Promoted Trophoblast Differentiationsupporting

confidence: 73%

Section: Cannabinoids Promoted Trophoblast Differentiationmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Stage-Specific Excitation of Cannabinoid Receptor Exhibits Differential Effects on Mouse Embryonic Development1

Wang

Paria

Dey

et al. 1999

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…This suggests a pathway in which PG stimulates cAMP synthesis to mediate activation though PKA. One important manifestation of activation is the early translocation of integrins α5β1 and α4β1 to the apical surface of abembryonic TE in mice [49,21,50]. Integrin α5β1-fibronectin engagement induces calmodulin-mediated calcium transients in the blastocyst, suggesting outside-in signalling [51].…”

Section: Embryo Growth Hatching and Activationmentioning

confidence: 99%

Trophoblast-uterine interactions at implantation

Aplin

Kimber

2004

Reprod Biol Endocrinol

152

View full text Add to dashboard Cite

Implantation of the embryo in the uterus is a critical and complex event and its failure is widely considered an impediment to improved success in assisted reproduction. Depending on whether placentation is invasive or superficial (epitheliochorial), the embryo may interact transiently or undergo a prolonged adhesive interaction with the uterine epithelium. Numerous candidate interactions have been identified, and there is good progress on identifying gene networks required for early placentation. However no molecular mechanisms for the epithelial phase are yet firmly established in any species. It is noteworthy that gene ablation in mice has so far failed to identify obligatory initial molecular events.

show abstract

“…However, results for the rate of induction of differentiation in embryos and TSC are different. Similarly after compaction, the embryo goes through preparation for adhesion to the uterine epithelium that first requires mRNA synthesis and then protein synthesis (Armant, 2005;Schultz et al, 1997). Developmental events in the early mouse embryo occur after zygotic genome activation at the two-cell stage.…”

Section: The "Idling Car" Hypothesismentioning

confidence: 99%

Interpreting the Stress Response of Early Mammalian Embryos and Their Stem Cells

Xie

Awonuga

Zhou

et al. 2011

International Review of Cell and Molecular Biology

View full text Add to dashboard Cite

Integrin trafficking regulates adhesion to fibronectin during differentiation of mouse peri-implantation blastocysts

Cited by 65 publications

References 51 publications

Stage-Specific Excitation of Cannabinoid Receptor Exhibits Differential Effects on Mouse Embryonic Development1

Stage-Specific Excitation of Cannabinoid Receptor Exhibits Differential Effects on Mouse Embryonic Development1

Trophoblast-uterine interactions at implantation

Interpreting the Stress Response of Early Mammalian Embryos and Their Stem Cells

Contact Info

Product

Resources

About