Integrin α5 and Integrin α4 cooperate to promote endocardial differentiation and heart morphogenesis

Schumacher, Jöerg; Wright, Zoë A.; Owen, Mackenzie L.; Bredemeier, Nina O.; Sumanas, Saulius

doi:10.1016/j.ydbio.2020.06.006

Cited by 12 publications

(4 citation statements)

References 54 publications

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“…However, the specific role and regulatory mechanism of TLN1 in CAD have not been reported yet. According to the String website [19], TLN1 was found to have a potential interaction with integrin alpha 5 (ITGA5), and ITGA5 is involved in promoting endocardial differentiation and cardiac morphogenesis [17].…”

Section: Introductionmentioning

confidence: 99%

TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction

Wang

Mao²,

2024

Folia Morphol

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Section: Introductionmentioning

confidence: 99%

TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction

Wang

Mao²,

2024

Folia Morphol

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“…Functional assessment in zebra sh demonstrates that when HBEGF expression is reduced with morpholino antisense reagents, it can result in a phenotype of myocardial contractile dysfunction [31]. It was reported that zebra sh Integrin alpha4 (itga4) and alpha5 function redundantly in cardiac morphogenesis and endocardial differentiation [32].…”

Section: Discussionmentioning

confidence: 99%

Piezo Type Mechanosensitive Ion Channel Component 1 is Required for Heart Development in Zebrafish

Liu¹,

Fang²,

Chen³

et al. 2021

Preprint

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PIEZO1 is a non-selective cation channel protein that converts mechanical stimuli into electrochemical signals through mechanical force transmission. In recent years, more and more attention has been paid to its relationship with cardiovascular development and related diseases. However, PIEZO1 is difficult to be used as a therapeutic target due to incomplete study of its related phenotype and mechanism. Starting with the phenotypes of zebrafish at different stages of development, piezo1 knockout zebrafish features decreased heart rate in embryonic stage, increased heart size in 72dpf larvae, as well as ventricular enlargement, passivation and increased immune infiltration in adult stage. Further characterization revealed the relationship between PIEZO1 and AcanA protein at embryonic stage, and hand2 protein at adult stage. Through further RNA-seq analysis, ptpn21 downregulation, shox2 upregulation, itga4 upregulation were used to explain ventricular enlargement, heart rate decrease and immune infiltration increase respectively. Overall, this study provides a theoretical basis for elucidating the mechanism of PIEZO1 in regulating cardiac development.

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“…Since CAFs express large amounts of fibronectin and are able to bind specifically to ITGA5 on ovarian cancer cells, they trigger cell clustering and communication. 32,39,40 The binding energy between fibronectin and ITGA5 was found to be −33.5 kcal/ mol by the molecular docking test, implying a strong interaction between fibronectin and ITGA5 with binding specificity (Figure 2F). Furthermore, SKOV3 cells were pretreated with RGD peptide before incubation with the CAF-probe.…”

Section: Caf-probe Traces Ovarian Cancer Cells By Targeting Itga5mentioning

confidence: 99%

Cancer-Associated Fibroblast Mimetic AIE Probe for Precision Imaging-Guided Full-Cycle Management of Ovarian Cancer Surgery

Dai,

Ouyang,

Wei

et al. 2023

Anal. Chem.

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Fluorescence imaging can improve surgical accuracy in ovarian cancer, but a high signal-to-noise ratio is crucial for tiny metastatic cancers. Meanwhile, intraoperative fluorescent surgical navigation modalities alone are still insufficient to completely remove ovarian cancer lesions, and the recurrence rate remains high. Here, we constructed a cancer-associated fibroblasts (CAFs)mimetic aggregation-induced emission (AIE) probe to enable fullcycle management of surgery that eliminates recurrence. AIE molecules (P3-PPh 3 ) were packed in hollow mesoporous silica nanoparticles (HMSNs) to form HMSN-probe and then coated with a CAFs membrane to prepare CAF-probe. First, due to the negative potential of the CAF-probe, the circulation time in vivo is elevated, which facilitates passive tumor targeting. Second, the CAF-probe avoids its clearance by the immune system and improves the bioavailability. Finally, the fibronectin on the CAF-probe specifically binds to integrin α-5 (ITGA5), which is highly expressed in ovarian cancer cells, enabling fluorescence imaging with a contrast of up to 8.6. CAF-probe-based fluorescence imaging is used to evaluate the size and location of ovarian cancer before surgery (preoperative evaluation), to guide tumor removal during surgery (intraoperative navigation), and to monitor tumor recurrence after surgery (postoperative monitoring), ultimately significantly improving the efficiency of surgery and completely eliminating tumor recurrence. In conclusion, we constructed a CAFs mimetic AIE probe and established a full-cycle surgical management model based on its precise imaging properties, which significantly reduced the recurrence of ovarian cancer.

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Integrin α5 and Integrin α4 cooperate to promote endocardial differentiation and heart morphogenesis

Cited by 12 publications

References 54 publications

TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction

TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction

Piezo Type Mechanosensitive Ion Channel Component 1 is Required for Heart Development in Zebrafish

Cancer-Associated Fibroblast Mimetic AIE Probe for Precision Imaging-Guided Full-Cycle Management of Ovarian Cancer Surgery

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