Inter- and Intra-specific Genetic Diversity in Diospyros Using SCoT and IRAP Markers

Guan, Changfei; Chachar, Sadaruddin; Zhang, Pingxian; Hu, Chaoqiong; Wang, Renzi; Yang, Yong

doi:10.1016/j.hpj.2019.12.005

Cited by 24 publications

(15 citation statements)

References 35 publications

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“…The level of polymorphic alleles in the D. kaki collection was also high, with P = 100% based on eight SSRs used, and comparable to other studies on Diospyros [ 6 , 14 , 16 ]. However, many markers were not efficient and SSRs are limited for Diospyros genotyping [ 19 , 41 ]. Therefore, it is still necessary to develop more efficient SSR markers for D. kaki and other Diospyros species that can be used for genotyping and evolutionary studies in artificial or natural populations.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, we observed the clear separation of D. kaki germplasm into two different clusters using SSR and ISSR markers. Another study on D. kaki germplasm using SSR, IRAP and SCoT markers separated 228 accessions into five genetic clusters mostly based on geographical origin [ 6 , 19 ]. In comparison, our results revealed lower cluster numbers in our D. kaki collection.…”

Section: Discussionmentioning

confidence: 99%

“…In Diospyros , molecular markers have been developed in recent years for the efficient characterization and further utilization of germplasm resources. Phylogenetic relationships of Diospyros species are mainly studied based on random amplification of polymorphic DNA (RAPD) [ 11 ], simple sequence repeats (SSRs) [ 12 , 13 , 14 , 15 , 16 ], internal transcribed spacer (ITS) [ 17 ], sequence-related amplified polymorphism (SRAP) [ 14 , 18 ], inter-retrotransposon-amplified polymorphism (IRAP) [ 19 ], start codon-targeted (SCoT) [ 19 , 20 , 21 ] or organelle sequences (orgDNA) [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity in Diospyros Germplasm in the Western Caucasus Based on SSR and ISSR Polymorphism

Самарина

Malyarovskaya

Reim

et al. 2021

Biology

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Persimmon germplasm in the Western Caucasus represent one of the most northerly collections. In our study, 51 commercial cultivars of D. kaki, 3 accessions of D. virginiana and 57 D. lotus accessions from six geographically distant populations were investigated using 19 microsatellite and 10 inter simple sequence repeat (ISSR) markers. After STRUCTURE analysis, the single accessions of Diospyros were allocated to three genetic clusters. Genetic admixtures in the important genotypes of D. kaki were revealed, whereas D. lotus accessions showed no admixture with other genetic clusters. The correspondence of genetic data and phenotypical traits was estimated in the D. kaki collection. The most frost tolerant genotypes of the collection, such as “Mountain Rogers”, “Nikitskaya Bordovaya”, “Rossiyanka”, “MVG Omarova”, “Meader”, “Costata”, “BBG”, and “Jiro”, showed a high percentage of genetic admixtures and were grouped close to D. virginiana. Some of these genotypes are known to be interspecific hybrids with D. virginiana. A low level of genetic diversity between the distant D. lotus populations was revealed and it can be speculated that D. lotus was introduced to the Western Caucasus from a single germplasm source. These results are an important basis for the implementation of conservation measures, developing breeding strategies, and improving breeding efficiency.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Diversity in Diospyros Germplasm in the Western Caucasus Based on SSR and ISSR Polymorphism

Самарина

Malyarovskaya

Reim

et al. 2021

Biology

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“…The SSR primers were the same as reported ( Guan et al, 2019 ; Guan et al, 2020 ) and modified with 3 fluorescent markers at the 5’-end, including FAM (6-carboxy-fluorescein), HEX (hexachloro-fluorescein), and TAMRA (carboxy tetramethyl-rhodamine). The UPGMA (unweighted pair group method with arithmetic mean) tree was performed on the DARwin software.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic Characterization of Postharvest Fruit Qualities in Astringent and Non-astringent Persimmon (Diospyros kaki) Cultivars

et al. 2021

Self Cite

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Phenotypic characterization of postharvest traits is essential for the breeding of high-quality fruits. To compare postharvest traits of different genetic lines, it is essential to use a reference point during fruit development that will be common to all the lines. In this study, we employed a non-destructive parameter of chlorophyll levels to establish a similar physiological age and compared several postharvest traits of ten astringent and seven non-astringent persimmon cultivars. The fruit’s traits examined were astringency, weight, total soluble solids (TSS), titratable acidity (TA), chlorophyll levels (IAD), color (hue), firmness, color development and firmness loss during storage, crack development, and susceptibility to Alternaria infection. Although the chlorophyll (IAD) index and color (hue) showed a high correlation among mature fruits of all cultivars, the chlorophyll parameter could detect higher variability in each cultivar, suggesting that IAD is a more rigorous parameter for detecting the developmental stage. The average weight, TSS, and TA were similar between astringent and non-astringent cultivars. Cracks appeared only on a few cultivars at harvest. Resistance to Alternaria infection and firmness were lower in astringent than in non-astringent cultivars. Only the astringent cultivar “32” was resistant to infection possibly due to the existence of an efficient peel barrier. It was concluded that a high correlation existed between astringency, susceptibility to Alternaria infection, and firmness. Cracks did not correlate with astringency or firmness. The phenotypic traits evaluated in this work can be used in future breeding programs for elite persimmon fruits.

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“…In addition to REMAP and ISSR, SCoT (start codon-targeted) markers together with IRAP has also been used to analyse relationships among samples. Guan et al (2020) investigate genetic diversity of 268 Diospyros accessions from different regions in China, determining 90 and 97 polymorphic alleles from nine SCoT and nine IRAP markers. Shehata et al (2020) performed different study in both yeast (Saccharomyces cerevisiae L.) and barley (Hordeum vulgare L.) by using IRAP and SCoT.…”

Section: Irap (Inter Retrotransposon Amplified Polymorphism)mentioning

confidence: 99%

Application of different molecular markers in biotechnology

Kocaman

Toy

Marakli

2020

International Journal of Science Letters

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Inter- and Intra-specific Genetic Diversity in Diospyros Using SCoT and IRAP Markers

Cited by 24 publications

References 35 publications

Genetic Diversity in Diospyros Germplasm in the Western Caucasus Based on SSR and ISSR Polymorphism

Genetic Diversity in Diospyros Germplasm in the Western Caucasus Based on SSR and ISSR Polymorphism

Phenotypic Characterization of Postharvest Fruit Qualities in Astringent and Non-astringent Persimmon (Diospyros kaki) Cultivars

Application of different molecular markers in biotechnology

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