Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

Ebrahimi, Mohsen; Farajpour, Mostafa; Rahimmalek, Mehdi

doi:10.4238/2012.august.27.1

Cited by 18 publications

(4 citation statements)

References 15 publications

(20 reference statements)

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“…ISSR markers are one of the most efficient marker systems because of their capacity to reveal several informative bands from a single amplification [20]. The marker has a high polymorphic degree among closely related varieties, along with high levels of repeatability and reliability which are efficiently used for assessing genetic variation in different crops such as Achillea species [21,22], melon [23], Zingiber officinale [24], and cowpea [25]. Shortages in water resources are expected sooner or later in many parts of the world due to climate change and overpopulation [26].…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Khodaee

Azizinezhad

Etminan

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Crop wild relatives (CWRs) are commonly used as a suitable genetic reservoir for plant breeding. They can be used for enhancing the tolerance of plant varieties to biotic and abiotic stresses. Studying the genetic diversity of related wheat species in Iran could be useful to improve different traits of bread wheat, since the country is one of the major centers of genetic diversity and distribution of Aegilops species. Therefore, the aim of the present study was to determine the relationship among 48 Aegilops triuncialis accessions using three DNA marker systems, including start codon targeted (SCoT), CAAT box-derived polymorphism (CBDP), and inter-simple sequence repeat (ISSR) markers. Results A total of 359 amplified DNA fragments were generated using 13 CBDP, 14 SCoT, and 16 ISSR primers that produced 96, 147, and 152 bands, respectively. The discriminating power of the three markers was assessed using polymorphism information content (PIC), marker index (MI), and resolving power (Rp). The mean values of PIC for ISSR, SCoT, and CBDP markers were 0.3, 0.26, and 0.34, respectively, indicating the efficiency of the three markers in detecting polymorphism among the studied accessions. ISSR markers had the highest values of MI, Rp, and polymorphism percentage as compared to SCoT and CBDP markers. Based on the Shannon index and heterozygosity values, genetic diversity in the Alborz population was more than in other populations. The accessions were classified into six, five, and five groups based on ISSR, SCoT, and CBDP using the UPGMA method. According to the results of cluster and PCoA analyses, the variation patterns corresponded with the geographical distribution of the Ae. triuncialis accessions. Conclusions The three markers provided a comprehensive pattern of the genetic diversity among the Iranian Ae. triuncialis accessions. This information could allow for a future insight into wheat breeding programs.

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Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Khodaee

Azizinezhad

Etminan

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…These results have a high paramount to the success of a breeding program that mainly depends on the availability of a broad genetic base. Also, the maintaining of genetic diversity is one of the most important objectives in preserving endangered and threatened plants (Akbari et al, 2018;Ebrahimi et al, 2012aEbrahimi et al, , 2012bFarajpour et al, 2011;Daneshvar, 2016a, 2016b;Khadivi-Khub et al, 2012). The knowledge of genetic diversity among populations gives some essential details in the formulation of suitable management strategies for preserving (Francisco-Ortega et al, 2000;Milligan et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

Morphological Characterization of Ficus religiosa Genotypes in Iran by Multivariate Analysis

Hesami¹,

Rahmati‐Joneidabad

2018

horts

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Ficus religiosa L. is one of the most popular species in the Moraceae family that is known as a multipurpose forest tree species because of its medicinal, ornamental, and religious value. F. religiosa is an important tree in South Asia, and it possesses various common names such as peepal, bodhi, bo tree, and asvattha. This species as a traditional tree is broadly planted as a roadside tree, and it plays an important role as a medicinal tree in various diseases such as asthma, stomatitis, diabetes, inflammations, glandular swelling disorders, and wound healing. Because F. religiosa is characterized as a subtropical tree, it fully grew in southern parts of Iran. The morphological variation of 72 individuals of F. religiosa from six southern regions of Iran was investigated based on multivariate analysis. Our results indicated that the highest tree, leaf, and petiole lengths, as well as leaf width, were observed in Kish and Qeshm genotypes, whereas the Chabahar genotype had the lowest petiole length. Results of simple correlation analysis showed the existence of significant positive and negative correlations among some important parameters. The highest correlation was observed between leaf, tree, and petiole lengths. Populations were clustered in four groups. The Kish and Qeshm genotypes were closely related to each other and differentiated from the Chabahar genotype. The whole dataset was subjected to principal component analysis (PCA). PCA showed that the first two factor components explained 84.51% of the variation and the first factor component had the positive relationship with leaf, tree, and petiole lengths.

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“…Based on the cluster analysis there was a moderate diversity among the four studied onion genotypes. These variation is due to genetic factors among the samples (Ebrahimi et al 2012;Farajpour et al 2017;Boroomand et al 2018;Hassanabadi et al 2019). Among the parameters, DM and POD activity had the highest positive correlation coefficient with each other (r= 0.76; P<0.01; Figure 5).…”

Section: Cluster and Correlation Analysesmentioning

confidence: 93%

Physiological Responses of Gamma-Irradiated Onion Bulbs during Storage

Ahmadi¹

2020

Tarım Bilimleri Dergisi

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The present study aimed to evaluate the effects of different doses of gamma radiation on some physiological characters of onion genotypes and the expression of PAL gene in the best interaction of gamma irradiation dose and onion genotype treatment. To this aim, four onion genotypes (White-Qom, White-Neyshabour, Red-Ridge-Lump, and Red-Ray-Corrugated) irradiated at 0, 30, 60, 90, 120, and 150 Gy. After four months of storage (at 10-15 °C and 70% relative humidity), the effects of gamma rays on the dry matter (DM), protein content, phenylalanine ammonia lyase (PAL) and peroxidase (POD) were investigated. In addition, the expression of PAL gene in the best interaction of gamma irradiation dose and onion genotype treatment was assessed. The result indicated that POD activity was increased by most of the gamma irradiation levels; however, the protein content and PAL activity were decreased. Moreover, dry matter content was found to be highly genotype-dependent. A linear regression (R 2 = 0.82) between PAL activity and gamma irradiation levels, was observed. PAL activity decreased with increasing in gamma irradiation level, while the expression rate of PAL gene was not significantly changed between irradiated and non-irradiated control, indicating that the radiation might not have direct effects on the gene regulatory elements. These results suggest that gamma irradiation could reduce the PAL activity possibly by controlling abiotic stress sources such as fungal and bacterial stresses.

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Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

Cited by 18 publications

References 15 publications

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Morphological Characterization of Ficus religiosa Genotypes in Iran by Multivariate Analysis

Physiological Responses of Gamma-Irradiated Onion Bulbs during Storage

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