Inter- and intra-varietal analysis of three<i>Olea europaea</i>L. cultivars using the RAPD technique

Gemas, V.J.V.; Rijo-Johansen, M. J.; Tenreiro, Rogério; Fevereiro, Pedro

doi:10.1080/14620316.2000.11511243

Cited by 34 publications

(18 citation statements)

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“…The technique was also employed by Gemas et al (2000) and by Roselli et al (2002) to evaluate intra-and inter-cultivar variability. Both RAPD and RFLP have contributed greatly to the taxonomic classification within the genus Olea (Besnard et al, 2002).…”

Section: Chronology Of the Proposed Classification Systemsmentioning

confidence: 99%

The classification of olive germplasm

Ganino

Bartolini

Fabbri

2006

The Journal of Horticultural Science and Biotechnology

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Section: Chronology Of the Proposed Classification Systemsmentioning

confidence: 99%

The classification of olive germplasm

Ganino

Bartolini

Fabbri

2006

The Journal of Horticultural Science and Biotechnology

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“…DNA-based markers provided a new opportunity for genetic characterisation and biodiversity studies in olive. In recent years random amplified polymorphic DNA (RAPD) markers have been extensively used in several studies of genetic variability in olive (Fabbri et al 1995;Mekuria et al 1999;Gemas et al 2000;Gonzalo-Claros et al 2000;Hess et al 2000;Belaj et al 2001Belaj et al , 2002Besnard et al 2001;Sanz-CortØs et al 2001), probably because of their relative advantages over other molecular techniques (Welsh and McClelland 1990;Williams et al 1990). Amplified fragment length polymorphism (AFLP) markers, developed by Vos et al (1995), have also been applied to study the genetic relationships among cultivated olives, wild forms and related species (Angiolillo et al 1999;Baldoni et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive

et al. 2003

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RAPDs, AFLPs and SSRs were compared in terms of their informativeness and efficiency in a study of genetic diversity and relationships among 32 olive cultivars cultivated in Italy and Spain. SSRs presented a higher level of polymorphism and a greater information content, as assessed by the expected heterozygosity, than AFLPs and RAPDs. The lowest values of expected heterozygosity were obtained for AFLPs, which, nevertheless were the most efficient marker system due to their capacity to reveal the highest number of bands per reaction and because of the high values achieved for a considerable number of indexes. All three techniques discriminated the genotypes very effectively, but only SSRs were able to discriminate the cultivars Frantoio and Cellina. The correlation coefficients of similarity were statistically significant for all three marker systems used but were lower for the SSR data than for RAPDs and AFLPs. For all markers a high similarity in dendrogram topologies was obtained although some differences were observed. All the dendrograms, including that obtained by the combined use of all the marker data, reflect some relationships for most of the cultivars according to their geographic diffusion. AMOVA analysis detected greater genetic differentiation among cultivars within each country than it did between the two countries.

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“…These have included isozymes (Ouazzani et al, 1993), chloroplast DNA RFLPs (Amane et al, 1999;Lumaret et al, 2000), AFLPs (Angiolillo et al, 1999;Baldoni et al, 2000), RAPDs (Bogani et al, 1994;Fabbri et al, 1995;Gemas et al, 2000;Besnard et al, 2001), microsatellites (Sefc et al, 2000;Rallo et al, 2000;Cipriani et al, 2002), and multiple markers (Hess et al, 2000;Belaj et al, 2003).…”

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confidence: 99%