Inter-Conversion of Carbohydrate Reserves from  Pollen Maturation to Rehydration in a Chili Pepper

García, Carolina Carrizo; Guarnieri, Massimo; Pacini, Ettore

doi:10.4236/ajps.2013.46146

Cited by 12 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rehydration of pollen is known to have consequences on the metabolic dynamics. A study with Capsicum annuum pollen showed that only 30 min incubation in an osmotic solution at room temperature led to significant pollen rehydration and changes in sugar content (García et al 2013 ). Furthermore, Ricinus communis seed, which, like pollen, is dry at maturity, showed marked metabolic changes upon rehydration at room temperature, with an accumulation of TCA metabolites (Ribeiro et al 2015 ).…”

Section: Resultsmentioning

confidence: 99%

The effect of isolation methods of tomato pollen on the results of metabolic profiling

et al. 2019

View full text Add to dashboard Cite

IntroductionUntargeted metabolomics is a powerful tool to detect hundreds of metabolites within a given tissue and to compare the metabolite composition of samples in a comprehensive manner. However, with regard to pollen research such comprehensive metabolomics approaches are yet not well developed. To enable isolation of pollen that is tightly enclosed within the anthers of the flower, such as immature pollen, the current pollen isolation protocols require the use of a watery solution. These protocols raise a number of concerns for their suitability in metabolomics analyses, in view of possible metabolic activities in the pollen and contamination with anther metabolites.ObjectivesWe assessed the effect of different sample preparation procedures currently used for pollen isolation for their suitability to perform metabolomics of tomato pollen.MethodsPollen were isolated using different methods and the metabolic profiles were analysed by liquid chromatography–mass spectrometry (LC–MS).ResultsOur results demonstrated that pollen isolation in a watery solution led to (i) rehydration of the pollen grains, inducing marked metabolic changes in flavonoids, phenylpropanoids and amino acids and thus resulting in a metabolite profile that did not reflect the one of mature dry pollen, (ii) hydrolysis of sucrose into glucose and fructose during subsequent metabolite extraction, unless the isolated and rehydrated pollen were lyophilized prior to extraction, and (iii) contamination with anther-specific metabolites, such as alkaloids, thus compromising the metabolic purity of the pollen fraction.ConclusionWe conclude that the current practices used to isolate pollen are suboptimal for metabolomics analyses and provide recommendations on how to improve the pollen isolation protocol, in order to obtain the most reliable metabolic profile from pollen tissue.Electronic supplementary materialThe online version of this article (10.1007/s11306-018-1471-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

The effect of isolation methods of tomato pollen on the results of metabolic profiling

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Drought and temperature stresses can severely affect starch accumulation, and absence of starch in mature pollen can be an indicator of pollen sterility [109,110,115,[190][191][192]. Endogenous starch is consumed during the first phases of pollen tube emission when pollen tube growth is at the expense of pollen reserves [193,194]. After this autotrophous phase, pollen grains obtain carbohydrates and other substances from the stigma and style.…”

Section: Adjustment Of Osmotic Pressure and Water Balance In Pollenmentioning

confidence: 99%

The Trials and Tribulations of the Plant Male Gametophyte — Understanding Reproductive Stage Stress Tolerance

Pacini¹

2016

Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives

Self Cite

View full text Add to dashboard Cite

Yield and productivity of many crop species depend on successful reproductive development to produce seeds or fruits for human nutrition. Plants determine the right time to flower based on environmental cues (day length, temperature) and angiosperms have evolved a plethora of mechanisms to adapt flowering to specific environmental conditions. Despite these adaptation mechanisms, fertilisation and seed production remain subject to the reigning weather conditions before and during flowering. To fertilise the immobile female gametes inside the ovule, the male gametophytes need to be dispersed in a hostile environment. In crop plants, unexpected inclement weather conditions during male gametophyte development and pollen dispersal are often associated with dramatic yield losses. Molecular and physiological studies are gradually making progress in identifying genes and processes that control various aspects of pollen development, but the many intricacies involved in environmental control of pollen development and-in particular-regulation of male fertility remain poorly understood. The aim of this paper is to draw attention to the enormous amount of complexity and biodiversity that exist in angiosperm male gametophyte development. A better understanding of the strategies that exist in adapting pollen production and fertility to environmental challenges may ultimately benefit improvement of abiotic stress tolerance in major food crops.

show abstract

“…Koch, 2004 reviewed the role of acid invertase in plants; Three different acid invertases can be identified according to their location: vacuolar invertase (VIN), cytoplasmic invertase (CIN) and cell wall invertase (CWIN). The function of acid invertases in reproductive tissues has been intensely studied in different species such as lily [ 39 , 40 ], tobacco [ 41 , 42 ], tomato [ 33 ] and chilli pepper [ 43 ].…”

Section: Metabolite Profiles During Pollen Developmentmentioning

confidence: 99%

The Metabolic Basis of Pollen Thermo-Tolerance: Perspectives for Breeding

Paupière

Heusden²,

Bovy³

2014

Metabolites

View full text Add to dashboard Cite

Crop production is highly sensitive to elevated temperatures. A rise of a few degrees above the optimum growing temperature can lead to a dramatic yield loss. A predicted increase of 1–3 degrees in the twenty first century urges breeders to develop thermo-tolerant crops which are tolerant to high temperatures. Breeding for thermo-tolerance is a challenge due to the low heritability of this trait. A better understanding of heat stress tolerance and the development of reliable methods to phenotype thermo-tolerance are key factors for a successful breeding approach. Plant reproduction is the most temperature-sensitive process in the plant life cycle. More precisely, pollen quality is strongly affected by heat stress conditions. High temperature leads to a decrease of pollen viability which is directly correlated with a loss of fruit production. The reduction in pollen viability is associated with changes in the level and composition of several (groups of) metabolites, which play an important role in pollen development, for example by contributing to pollen nutrition or by providing protection to environmental stresses. This review aims to underline the importance of maintaining metabolite homeostasis during pollen development, in order to produce mature and fertile pollen under high temperature. The review will give an overview of the current state of the art on the role of various pollen metabolites in pollen homeostasis and thermo-tolerance. Their possible use as metabolic markers to assist breeding programs for plant thermo-tolerance will be discussed.

show abstract

Inter-Conversion of Carbohydrate Reserves from Pollen Maturation to Rehydration in a Chili Pepper

Cited by 12 publications

References 23 publications

The effect of isolation methods of tomato pollen on the results of metabolic profiling

The effect of isolation methods of tomato pollen on the results of metabolic profiling

The Trials and Tribulations of the Plant Male Gametophyte — Understanding Reproductive Stage Stress Tolerance

The Metabolic Basis of Pollen Thermo-Tolerance: Perspectives for Breeding

Contact Info

Product

Resources

About