Inter-generic mitochondrial genome recombination in somatic hybrids between<i>Citrus</i>and<i>Poncirus</i>

Cheng, Yunjiang; Guo, Wenkai; Deng, Xiuxin

doi:10.1080/14620316.2007.11512317

Cited by 2 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The conservation of a single parental fragment suggested homologous recombination. Such rearrangements of the mitochondrial genome have also been reported in some studies following asymmetric and symmetric somatic hybridizations in citrus [38][39][40][41]84,88].…”

Section: Inheritance Of Cytoplasmic Genomessupporting

confidence: 64%

“…In the case of leaf and callus protoplast fusion, most publications report inheritance of the callus mitochondria and a random uniparental inheritance for the chloroplast (Ollitrault et al [37] for review). However, a few works have reported potential recombination of the mitochondrial genome [38][39][40][41]. For the nuclear genome, only a few markers have generally been used to confirm the hybridity of tetraploid plants.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genomic Instability in Somatic Hybridization between Poncirus and Citrus Species Aiming to Create New Rootstocks

et al. 2022

View full text Add to dashboard Cite

Rootstocks are an important component for citrus adaptation to increasing biotic and abiotic stresses resulting from global climate change. There is a strong complementarity between Citrus species, which adapt to abiotic stresses, and Poncirus trifoliata and its intergeneric hybrids, which exhibit resistances or tolerances to major diseases and pests. Thus, symmetrical somatic hybridization between complementary diploid rootstocks of these two genera appears to be an efficient way to develop new tetraploid rootstocks in order to address the new challenges of the citrus industry. New intergeneric somatic hybrids were obtained by electrofusion between protoplasts of Citrus and P. trifoliata hybrids. Extensive characterization of the nuclear and cytoplasmic genomes was performed by genotyping-by-sequencing (GBS) analysis. This revealed diploid cybrids and nuclear somatic hybrids. Mitochondrial genomes were mostly inherited from the callus parent, but homologous recombination events were observed for one parental combination. Chloroplasts exhibited random uniparental inheritance. GBS revealed local chromosomal instabilities for all nuclear somatic hybrids and whole chromosome eliminations for two hybrids. However, at the whole genome level, symmetrical addition of the nuclear genomes of both parents was predominant and all somatic hybrids displayed at least one trifoliate orange haplotype throughout the genome.

show abstract