Interação do Trypanosoma cruzi com diferentes vetores: uso para o xenodiagnóstico

Alvarenga, Nelson J.; Bronfen, Elizabeth

doi:10.1590/s0037-86821984000300007

Cited by 10 publications

(6 citation statements)

References 3 publications

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“…Different approaches have been studied in considering cycle of T. cruzi and vectors (Dias 1934, Wood 1960, Zeledon 1974, Coura 1975, Alvarenga & Bronfen 1984, PerlowagoraSzumlewicz et al 1990.…”

Section: Relationship Between Cycle Of the Parasite And The Vectormentioning

confidence: 99%

Xenodiagnosis

Schenone

1999

Mem. Inst. Oswaldo Cruz

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Section: Relationship Between Cycle Of the Parasite And The Vectormentioning

confidence: 99%

Xenodiagnosis

Schenone

1999

Mem. Inst. Oswaldo Cruz

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“…However, all that has been said is not to undermine the investigation that discovered an insect in which T. cruzi undergoes an exceptional high rate of epimastigotes-to--metacyclic trypomastigotes transformation, as described in 1984 by Alvarenga & Bronfen 1 . Although this finding has not proved particularly useful in the choice of xenodiagnostic agent candidates, it is of major importance to investigators involved in classification of T. cruzi strains on the basis of isoenzyme profiles reported by Miles & Cibulskis 10 , in the analysis of infection of bugs with single-cell-isolate clones of T. cruzi, described by Garcia & Dvorak 7 and also for the characterization of parasites on the genotype level at least until new techniques that would not require parasite population amplification are developed, as stated in 1986 by Morel et al 12 .…”

Section: And Recomendationsmentioning

confidence: 99%

Studies in search of a suitable experimental insect model for xenodiagnosis of hosts with Chagas' disease: 3 - On the interaction of vector species and parasite strain in the reaction of bugs to infection by Trypanosoma cruzi

Perlowagora-Szumlewicz¹,

Müller²,

Moreira³

1988

Rev. Saúde Pública

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ABSTRACT:The reaction of nine vector species of Chagas' disease to infection by seven different Trypanosoma cruzi strains; Berenice, Y, FL, CL, S. Felipe, Colombiana and Gávea, are examined and compared. On the basis of the insects' ability to establish and maintain the infection, vector species could be divided into two distinct groups which differ in their reaction to an acute infection by T. cruzi. While the proportion of positive bugs was found to be low in Triatoma infestans and Triatoma dimidiata it was high, ranging from 96.9% to 100% in the group of wild (Rhodnius neglectus, Triatoma rubrovaria)and essentially sylvatic vectors in process of adaptation to human dwellings, maintained under control following successful insecticidal elimination of Triatoma infestans (Panstrongylus megistus, Triatoma sordida and Triatoma pseudomaculata). An intermediate position is held by Triatoma brasiliensis and Rhodnius prolixus. This latter has been found to interchange between domestic and sylvatic environments. The most important finding is the strikingly good reaction between each species of the sylvatic bugs and practically all T. cruzi strains herein studied, thus indicating that the factors responsible for the excellent reaction of P.megistus to infection by Y strain, as previously reported also come into operation in the reaction of the same vector species to acute infections by five of the remaining T.cruzi strains. Comparison or data reported by other investigators with those herein described form the basis of the discussion of Dipetalogaster maximus as regards its superiority as a xenodiagnostic agent.

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“…The natural populations of T. cruzi are polyclonal and its subpopulations can be exposed to selective pressure depending on the genetic constitution of the vertebrate host 10,11 , different species of triatomines in which the parasites develop and circulate 12,13 , and genetic constitution of these parasites 14 . However, some aspects of the coevolution of this parasite and vector bugs remain poorly understood 15 .…”

Section: Introductionmentioning

confidence: 99%

Sympatry influence in the interaction of Trypanosoma cruzi with triatomine

Dworak

Araújo

Gomes

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Trypanosoma cruzi, the etiologic agent of Chagas disease, is widely distributed in nature, circulating between triatomine bugs and sylvatic mammals, and has large genetic diversity. Both the vector species and the genetic lineages of T. cruzi present a varied geographical distribution. This study aimed to verify the influence of sympatry in the interaction of T. cruzi with triatomines. Methods: The behavior of the strains PR2256 (T. cruzi II) and AM14 (T. cruzi IV) was studied in Triatoma sordida (TS) and Rhodnius robustus (RR). Eleven fifth-stage nymphs were fed by artificial xenodiagnosis with 5.6 × 10 3 blood trypomastigotes/0.1mL of each T. cruzi strain. Every 20 days, their excreta were examined for up to 100 days, and every 30 days, the intestinal content was examined for up to 120 days, by parasitological (fresh examination and differential count with Giemsa-stained smears) and molecular (PCR) methods. Rates of infectivity, metacyclogenesis and mortality, and mean number of parasites per insect and of excreted parasites were determined. Results: Sympatric groups RR+AM14 and TS+PR2256 showed higher values of the four parameters, except for mortality rate, which was higher (27.3%) in the TS+AM14 group. General infectivity was 72.7%, which was mainly proven by PCR, showing the following decreasing order: RR+AM14 (100%), TS+PR2256 (81.8%), RR+PR2256 (72.7%) and TS+AM14 (36.4%). Conclusions: Our working hypothesis was confirmed once higher infectivity and vector capacity (flagellate production and elimination of infective metacyclic forms) were recorded in the groups that contained sympatric T. cruzi lineages and triatomine species.

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Interação do Trypanosoma cruzi com diferentes vetores: uso para o xenodiagnóstico

Cited by 10 publications

References 3 publications

Xenodiagnosis

Xenodiagnosis

Studies in search of a suitable experimental insect model for xenodiagnosis of hosts with Chagas' disease: 3 - On the interaction of vector species and parasite strain in the reaction of bugs to infection by Trypanosoma cruzi

Sympatry influence in the interaction of Trypanosoma cruzi with triatomine

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