Interaction between CTLA4 gene and IBD5 locus in Hungarian Crohn’s disease patients

Crohn's disease (CD) and ulcerative colitis (UC) are the major types of inflammatory bowel disease (IBD) and exhibit similar clinical features and epidemiology. The main objective of this study was to analyze the correlation between the CTLA-4 gene +49A/G polymorphism and the NOD2/CARD15 gene N852S polymorphism in Turkish patients with IBD using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. In this study, we evaluated the frequency of the CTLA-4 (+49A/G) and NOD2/CARD15 (N852S) polymorphisms in 62 patients with CD, 76 patients with UC, and 152 healthy individuals. The CTLA-4 and NOD2/CARD15 variants, rs231775 and rs104895467, were genotyped by PCR followed by RFLP. The results for the patients and the control group were statistically analyzed. According to our results, the CTLA-4 gene +49A/G polymorphism AA genotype was prevalent in CD patients and controls (29% vs 40%); the AG (56% vs 51%) and GG (15% vs 9%) genotypes were also observed. The prevalence of the of AA, AG and GG genotypes for the +49A/G polymorphism was 56%, 32% and 12%, respectively, in the UC patients, and 40%, 51% and 9%, respectively, in the healthy controls. In all subjects, just one band of 151 bp, corresponding to wild-type N852S, was found, and no other N852S mutant bands (151+129+22 and 129+22 bp) were detected using PCR-RFLP fragment electrophoresis.The CTLA-4 gene +49 A/G polymorphism and the NOD2/CARD15 gene N852S polymorphism were not associated with CD or UC in a Turkish population.

show abstract

CTLA-4 Gene +49 A/G Polymorphism in Prostate Cancer Patients in Turkish Population

Diler¹

2017

ACP

View full text Add to dashboard Cite

Background: Cytotoxic T-lymphocyte antigen 4 (CTLA-4), the T cell surface antigen is expressed during activation. Prostate cancer (PCa) is one of the most common cancers in Western population and its rate is increasing in the Eastern World. The aim of this study was to evaluate cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene+49A/G polymorphisms in prostate cancer patients. Material and methods:This study included 119 (mean age: 68.94±8.38 years) healthy controls and 62 (mean age: 72.83±7.60 years) patients with prostate cancer. The CTLA-4 gene+49A/G (rs231775) gene regions were amplified using polymerase chain reaction (PCR), detected by restriction fragment length polymorphism (RFLP).

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Interaction between CTLA4 gene and IBD5 locus in Hungarian Crohn’s disease patients

Abstract: Analysis of specific genotype combinations unfolded a possible association between the CTLA4 +49 A/G substitution and two of the observed IBD5 variants with respect to disease risk.

Cited by 4 publications

References 70 publications

Association of CTLA-4 variants with susceptibility to inflammatory bowel disease: A meta-analysis

Association of CTLA-4 variants with susceptibility to inflammatory bowel disease: A meta-analysis

CTLA-4 (+49A/G) and NOD2/CARD15 (N852S) polymorphisms with inflammatory bowel disease in Turkish patients

CTLA-4 Gene +49 A/G Polymorphism in Prostate Cancer Patients in Turkish Population

Contact Info

Product

Resources

About