Interaction between Food Substances and the Intestinal Epithelium

Shimizu, Makoto

doi:10.1271/bbb.90730

Cited by 130 publications

(97 citation statements)

References 82 publications

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“…Recent studies have shown that the intestinal functions are also affected by food factors that modulate the TJ. 28,29 The integrity of the confluent epithelial cell layer was shown by measuring the Trans Epithelial Electric Resistance (TEER) which reached a maximal level after one week (Fig. 2b).…”

Section: First Experimental Resultsmentioning

confidence: 99%

NutriChip: nutrition analysis meets microfluidics

et al. 2013

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This focus article introduces the concept of NutriChip, an integrated microfluidic platform for investigating the potential of the immuno-modulatory function of dairy food. The core component of the NutriChip is a miniaturized artificial human gastrointestinal tract (GIT), which consists of a confluent layer of epithelial cells separated from a co-culture of immune cells by a permeable membrane. This setting creates conditions mimicking the human GIT and allows studying processes that characterize the passage of nutrients though the human GIT, including the activation of immune cells in response to the transfer of nutrients across the epithelial layer. The NutriChip project started by developing a biologically active in vitro cellular system in a commercial Transwell co-culture system. This Transwell system serves as a reference for the micro-scale device which is being developed. The microfluidic setup of NutriChip allows monitoring of the response of immune cells to pro-inflammatory stimuli, such as lipid polysaccharide (LPS), and to the application of potentially anti-inflammatory dairy food. This differential response will be quantified by measuring the variation in expression of pro-inflammatory cytokines, including interleukin 1 (IL-1) and interleukin 6 (IL-6), secreted by the immune cells, and this is achieved by using a dedicated optical imager. A series of dairy products will be screened for their anti-inflammatory properties using the NutriChip system and, finally, the outcome of the NutriChip will be validated by a human nutrition trial.Therefore, the NutriChip platform offers a new option to evaluate the influence of food quality on health, by monitoring the expression of relevant immune cell biomarkers.

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Section: First Experimental Resultsmentioning

confidence: 99%

NutriChip: nutrition analysis meets microfluidics

et al. 2013

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“…To study mechanisms and effects of microbiota, microbiota metabolites, food digesta and bioactive food components on the barrier function of the intestinal epithelium (Shimizu 2010 ). To elucidate pathways for the transport of drugs or food components (e.g.…”

Section: Applicationsmentioning

confidence: 99%

Caco-2 Cell Line

Lea

2015

The Impact of Food Bioactives on Health

215

160

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The human epithelial cell line Caco-2 has been widely used as a model of the intestinal epithelial barrier. The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with brush border layer as found in the small intestine. The Caco-2 cell line is heterogeneous and contains cells with slightly different properties. Thus, cultivation conditions can be expected to select for the growth of subpopulations of cells resulting in a cellular model system with properties that may differ from the original cell line. Accordingly, results obtained under similar experimental conditions in different laboratories may not be directly comparable. Due to this, a variety of cloned Caco-2 cell lines has been established, and described in the literature. This chapter will however, focus on describing how to handle and cultivate the original Caco-2 cell line as obtained from cell culture collections like American Type Culture Collection and the European Collection of Cell Cultures. Detailed protocols for handling the Caco-2 cells in the laboratory are provided. Furthermore, in Chap. 9 general protocols for measuring barrier function by transepithelial resistance (TEER), and monolayer integrity by Lucifer Yellow fl ux are described. Proper testing of the cell monolayer is absolutely critical in exploiting Caco-2 cells to measure interaction, uptake and cellular transport of drugs and food components.

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“…Epithelial cellsFresponsible for mucosal permeabilityFrepresent a barrier to external stressors (eg, products of intestinal fermentation), whereas lamina propriaFin which vessels and immune cells resideFis more susceptible to internal stressors (eg, inflammation). [29][30][31][32][33] Results are summarized in Table 2 and Figure 4. Hsp60 positivity was localized in the cytoplasm and the greatest numbers of positive cells were consistently found in the epithelium.…”

Section: Immunohistochemistry Of Hsp60mentioning

confidence: 99%

Changes in Immunohistochemical Levels and Subcellular Localization After Therapy and Correlation and Colocalization With CD68 Suggest a Pathogenetic Role of Hsp60 in Ulcerative Colitis

Tomasello

Rodolico²,

Zerilli³

et al. 2011

Applied Immunohistochemistry &Amp; Molecular Morphology

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Abstract:In an earlier work, the role of heat shock protein (Hsp60) in the pathogenesis of ulcerative colitis (UC) was suggested by its significant increase in the pathological mucosa parallel with an increase in inflammatory cells. More data in this direction are reported in this work. We analyzed by immunohistochemistry biopsies of colon tissue from 2 groups of patients with UC and treated with either 5-aminosalicylic acid (5-ASA) alone or in combination with a probiotic. We looked for inflammatory markers and Hsp60. Both the treatments were effective in reducing symptoms but the group treated with both 5-ASA and probiotics showed better clinical results. Amelioration of symptoms was associated with reduction of both inflammation and Hsp60, a reduction that was most marked in the group treated with 5-ASA and probiotics. The levels of Hsp60 positively correlated with those of CD68-positive cells, and double immunofluorescence showed a high index of colocalization of the chaperonin and CD68 in lamina propria. Immunoelectron microscopy showed that Hsp60Fclassically a mitochondrial proteinFwas abundantly also present in cytosol in biopsies taken at the time of diagnosis, but not after the treatment. Our data suggest that Hsp60 is an active player in pathogenesis of UC and it can be hypothesized that the chaperonin is responsible, at least in part, for initiation and maintenance of disease.

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Interaction between Food Substances and the Intestinal Epithelium

Cited by 130 publications

References 82 publications

NutriChip: nutrition analysis meets microfluidics

NutriChip: nutrition analysis meets microfluidics

Caco-2 Cell Line

Changes in Immunohistochemical Levels and Subcellular Localization After Therapy and Correlation and Colocalization With CD68 Suggest a Pathogenetic Role of Hsp60 in Ulcerative Colitis

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