Interaction between graphene oxide and nitrogen-fixing bacterium Azotobacter chroococcum: Transformation, toxicity and nitrogen fixation

Yilihamu, Ailimire; Ouyang, Bowei; Ouyang, Peng; Bai, Yitong; Zhang, Qiangqiang; Shi, M.; Guan, Xin; Yang, Shengrong

doi:10.1016/j.carbon.2020.01.014

Cited by 28 publications

(11 citation statements)

References 48 publications

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“…Zhao et al found that GO significantly induced oxidative stress and genotoxicity in Eisenia fetida, resulting in lipid peroxidation, decreased lysosomal membrane stability, and DNA damage [8]. Furthermore, in the bacterium Azotobacter chroococcum, GO induced cell death in a dose-dependent manner owing to membrane damage and oxidative stress [9]. Due to its high dispersibility and transformation and abundant functional groups, GO inhibited the growth of freshwater algae through indirect toxicity via the shading effect and nutrient depletion [10].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli

Qiang

Zhang

et al. 2021

Nanomaterials

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The biological and environmental toxicity of graphene and graphene derivatives have attracted great research interest due to their increasing applications. However, the cytotoxic mechanism is poorly understood. Here, we investigated the cytotoxic effect of graphene oxide nanoribbons (GORs) on Escherichia coli (E. coli) in an in vitro method. The fabricated GORs formed long ribbons, 200 nm wide. Based on the results of the MTT assay and plate-culture experiments, GORs significantly inhibited the growth and reproduction of E. coli in a concentration-dependent manner. We found that GORs stimulated E. coli to secrete reactive oxygen species, which then oxidized and damaged the bacterial cell membrane. Moreover, interaction between GORs and E. coli cytomembrane resulted in polysaccharide adsorption by GORs and the release of lactic dehydrogenase. Furthermore, GORs effectively depleted the metal ions as nutrients in the culture medium by adsorption. Notably, mechanical cutting by GORs was not obvious, which is quite different from the case of graphene oxide sheets to E. coli.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli

Qiang

Zhang

et al. 2021

Nanomaterials

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“…In addition to the effects on bacterial growth and diversity, exposure to GBMs was shown to influence microbial activities and could potentially lead to disturbances of carbon or nitrogen cycles ( Zhou et al, 2019 ; Yilihamu et al, 2020 ). For example, previous studies indicated that exposure to GO or rGO could enhance the anaerobic ammonium oxidation activity that is involved in nitrogen removal in ecosystems ( Wang et al, 2013 ; Yin et al, 2015 ; Tomaszewski et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Graphene-Based Nanomaterials Modulate Internal Biofilm Interactions and Microbial Diversity

et al. 2021

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Graphene-based nanomaterials (GBMs), such as graphene oxide (GO) and reduced graphene oxide (rGO), possess unique properties triggering high expectations for the development of new technological applications and are forecasted to be produced at industrial-scale. This raises the question of potential adverse outcomes on living organisms and especially toward microorganisms constituting the basis of the trophic chain in ecosystems. However, investigations on GBMs toxicity were performed on various microorganisms using single species that are helpful to determine toxicity mechanisms but fail to predict the consequences of the observed effects at a larger organization scale. Thus, this study focuses on the ecotoxicological assessment of GO and rGO toward a biofilm composed of the diatom Nitzschia palea associated to a bacterial consortium. After 48 and 144 h of exposure to these GBMs at 0, 0.1, 1, and 10 mg.L−1, their effects on the diatom physiology, the structure, and the metabolism of bacterial communities were measured through the use of flow cytometry, 16S amplicon sequencing, and Biolog ecoplates, respectively. The exposure to both of these GBMs stimulated the diatom growth. Besides, GO exerted strong bacterial growth inhibition as from 1 mg.L−1, influenced the taxonomic composition of diatom-associated bacterial consortium, and increased transiently the bacterial activity related to carbon cycling, with weak toxicity toward the diatom. On the contrary, rGO was shown to exert a weaker toxicity toward the bacterial consortium, whereas it influenced more strongly the diatom physiology. When compared to the results from the literature using single species tests, our study suggests that diatoms benefited from diatom-bacteria interactions and that the biofilm was able to maintain or recover its carbon-related metabolic activities when exposed to GBMs.

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“…Nitrogen fixation can be defined as the process by which nitrogen present in the atmosphere is converted to ammonia by nitrogen-fixing bacteria for plants to utilize ( Yilihamu et al, 2020 ). Nitrogen is among the essential ingredients needed by the tomato plant.…”

Section: Macroelement Solubilization In the Rhizospherementioning

confidence: 99%

The application of plant growth-promoting rhizobacteria in Solanum lycopersicum production in the agricultural system: a review

Adedayo

Babalola

Prigent‐Combaret

et al. 2022

PeerJ

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Food safety is a significant challenge worldwide, from plantation to cultivation, especially for perishable products such as tomatoes. New eco-friendly strategies are needed, and beneficial microorganisms might be a sustainable solution. This study demonstrates bacteria activity in the tomato plant rhizosphere. Further, it investigates the rhizobacteria’s structure, function, and diversity in soil. Rhizobacteria that promote the growth and development of tomato plants are referred to as plant growth-promoting bacteria (PGPR). They form a series of associations with plants and other organisms in the soil through a mutualistic relationship where both parties benefit from living together. It implies the antagonistic activities of the rhizobacteria to deter pathogens from invading tomato plants through their roots. Some PGPR are regarded as biological control agents that hinder the development of spoilage organisms and can act as an alternative for agricultural chemicals that may be detrimental to the health of humans, animals, and some of the beneficial microbes in the rhizosphere soil. These bacteria also help tomato plants acquire essential nutrients like potassium (K), magnesium (Mg), phosphorus (P), and nitrogen (N). Some rhizobacteria may offer a solution to low tomato production and help tackle food insecurity and farming problems. In this review, an overview of soil-inhabiting rhizobacteria focused on improving the sustainable production of Solanum lycopersicum.

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Interaction between graphene oxide and nitrogen-fixing bacterium Azotobacter chroococcum: Transformation, toxicity and nitrogen fixation

Cited by 28 publications

References 48 publications

Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli

Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli

Graphene-Based Nanomaterials Modulate Internal Biofilm Interactions and Microbial Diversity

The application of plant growth-promoting rhizobacteria in Solanum lycopersicum production in the agricultural system: a review

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