Interaction between Manduca sexta allatotropin and Manduca sexta allatostatin in the fall armyworm Spodoptera frugiperda

Oeh, Uwe; Lorenz, Matthias; Dyker, Hubert; Lösel, Peter; Hoffmann, Klaus H.

doi:10.1016/s0965-1748(00)00043-6

Cited by 63 publications

(60 citation statements)

References 38 publications

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“…These maximal Y. Li and others levels of stimulation for Aedes-AT were similar to those previously found for Mas-AT during in vitro experiments using CA of various insects: up to 2.2-fold increase of JH in A. mellifera , up to three times increase in larval L. oleracea , a 2.6-fold increase in sugar-fed adult females of P. regina (Tu et al, 2001) and up to a 6-fold increase in adult M. sexta (Kataoka et al, 1989). However, the maximal level of stimulation by Aedes-AT in A. aegypti was lower than those by Mas-AT in either S. frugiperda (approximately 7-fold; Oeh et al, 2000) or in day 0 (approximately 15-fold) and day 6 (approximately 10-fold) adult virgin female of P. unipuncta (Koladich et al, 2002). In adult sugar-fed virgin A. aegypti, Aedes-AT exhibited a stage-specific stimulation of JH biosynthesis in a dosedependent manner, with a maximal concentration of approximately 10 -8 -10 -9 ·mol·l -1 ; a concentration similar to the titer of the peptide found in the hemolymph of most insect species [10 -6 mol l -1 in P. unipuncta and 10 -8 mol l -1 in L. oleracea and P. regina Koladich et al, 2002;Tu et al, 2001)].…”

Section: Responsiveness Of Corpora Allata Complex To Aedes-atmentioning

confidence: 82%

“…This is the Manduca sexta allatotropin (Mas-AT) identified from heads of the pharate adult tobacco hornworm Manduca sexta (Kataoka et al, 1989), a 13-residue amidated peptide with the sequence GFKNVEMMTARGFamide that stimulates adult CA in vitro but does not affect the activity of larval or pupal CA (Kataoka et al, 1989). Mas-AT was also purified from the methanolic brain extracts of the fall armyworm Spodoptera frugiperda (Oeh et al, 2000). It also stimulates JH biosynthesis in other lepidopteran species, such as the tobacco budworm Heliothis virescens (Kataoka et al, 1989), the tomato moth Lacanobia oleracea (Audsley et al, 1999 and S. frugiperda (Oeh et al, 2000), and non-lepidopteran species, such as A. mellifera larvae and the black blowfly Phormia regina (Tu et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Mas-AT was also purified from the methanolic brain extracts of the fall armyworm Spodoptera frugiperda (Oeh et al, 2000). It also stimulates JH biosynthesis in other lepidopteran species, such as the tobacco budworm Heliothis virescens (Kataoka et al, 1989), the tomato moth Lacanobia oleracea (Audsley et al, 1999 and S. frugiperda (Oeh et al, 2000), and non-lepidopteran species, such as A. mellifera larvae and the black blowfly Phormia regina (Tu et al, 2001). By use of immunochemical and molecular techniques, Mas-AT has been shown to be present in the brain of larval L. oleracea and the cotton leafworm Spodoptera littoralis (Audsley et al, 1999, the abdominal nervous system of the cockroaches Leucophaea maderae and Periplaneta americana (Rudwall et al, 2000), the true armyworm moth Pseudaletia unipuncta (Truesdell et al, 2000), P. regina, the adult large milk-weed bug Oncopeltus fasciatus, the adult oriental fruit fly Dacus dorsalis, larval and adult M. loreyi and the larval tea silk moth Andraca bipunctata (Tu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedesallatotropin

Unnithan

Veenstra

et al. 2003

Journal of Experimental Biology

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SUMMARY Previous studies have demonstrated that the synthesis of juvenile hormone(JH) by the isolated corpora allata (CA) complex in vitro as well as the JH titer in the yellow fever mosquito Aedes aegypti are elevated before feeding and low after a blood meal. In the present study, we used an in vitro radiochemical assay to analyze the effect of farnesoic acid(FA) and Aedes allatotropin (Aedes-AT) on the biosynthesis of JH and methyl farnesoate (MF) by the isolated CA complex of A. aegypti adult female. CA complex from day-0 females (0–1 h after emergence) exhibited a low basal juvenile hormone III (JH III) biosynthetic activity and did not respond to either allatotropic or FA stimulation. However, incubation of CA complexes from newly emerged females with Aedes-AT plus FA resulted in very high production of JH III. This is the first report suggesting that allatotropin makes corpora allata in newly emerged females capable for JH biosynthesis. When we studied CA complexes dissected from females 1 day after emergence, the stimulatory action of Aedes-AT was strong and dose-dependent,with maximum stimulation in the range of 10–8–10–9 mol l–1,suggesting that Aedes-AT is indeed a true allatotropin (a molecule with allatotropic activity) in A. aegypti. The addition to the culture medium of 40 μmol l–1 FA, a JH precursor, resulted in a 9-fold increase in JH III biosynthesis in 2-, 4- and 6-day-old sugar-fed females. The two major labeled products synthesized by the stimulated CA complex were identified as JH III and MF by RP-HPLC and GC–MS. Treatment of CA complexes with FA, but not Aedes-AT, resulted in an increase in MF. Application of both Aedes-AT and FA to the CA complexes of 2-, 4- and 6-day-old females resulted in the same effects as FA alone. These data suggest that in sugar-fed females, FA and Aedes-AT exert different effects on the terminal steps in JH biosynthesis.

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Section: Responsiveness Of Corpora Allata Complex To Aedes-atmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedesallatotropin

Unnithan

Veenstra

et al. 2003

Journal of Experimental Biology

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“…In contrast, only one type of AT has been isolated and functionally characterized; this AT was originally identified from the heads of pharate adult Manduca sexta (Mas-AT), with analogs being isolated from brain of Spodoptera frugiperda (Kataoka et al 1989;Oeh et al 2000), from the male accessory glands of Locusta migratoria (Paemen et al 1991), and from the mosquito Aedes aegypti (Veenstra and Costes 1999;Li et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus

Hernández‐Martínez

Lanz‐Mendoza

et al. 2005

Cell Tissue Res

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Confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-A (AS-A), allatostatin-C (AS-C) and allatotropin (AT), in adult female mosquitoes of Aedes aegypti and Anopheles albimanus. The specific patterns of immunostaining for each of the three peptides were similar in both species. The antisera raised against AT, AS-A, and AS-C revealed intense immunoreactivity in the cells of each protocerebral lobe of the brain and stained cells in each of the ventral ganglia and neuronal projections innervating various thoracic and abdominal tissues. Only the AS-A antiserum labeled immunoreactive endocrine cells in the midgut. The distribution of the peptides supports the concept that they play multiple regulatory roles in both species.

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“…In the fall armyworm (Spodoptera frugiperda (J.E. Smith, 1797)), Manse-PISCF/AST at 1 µmol/L had no effect on JH biosynthesis in day-2 adult females but could act to inhibit CA that were previously stimulated to produce higher levels of JH (Oeh et al 2000). The first gene for PISCF/AST was cloned from P. unipuncta and was found to encode a precursor protein that upon cleavage would release a single peptide identical to Manse-PISCF/ AST (Jansons et al 1996).…”

Section: Piscf/astsmentioning

confidence: 99%

Families of allatoregulator sequences: a 2011 perspective¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

BendenaWilliam

TobeStephen

2012

Can. J. Zool.

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Three different peptide families have been named "allatostatins" (ASTs), based on their initial purifications which were based on their ability to inhibit juvenile hormone (JH) biosynthesis. These include (i) a family of peptides that have a consensus C-terminal sequence Y/FXFGL-NH 2 ; (ii) a family of peptides with a conserved C-terminal sequence W(X) 6 W-NH 2 ; and(iii) a family of peptides with C-terminal sequence PISCF, some of which are C-terminally-amidated. Each allatostatin family has functions distinct and apart from the inhibition of JH biosynthesis. A peptide family known as the "allatotropins" serve to stimulate JH biosynthesis. This family of peptides also has been proven to exert multiple effects dependent on the species in question. Genome and peptidome projects are uncovering new members of these families and it is clear that these structures are not just confined to Insecta but are found in a range of invertebrates. The receptors for these neuropeptides have been identified and tested experimentally for specific ligand binding. The Y/FXFGLa-ASTs exert their action through galanin-like receptors, W(X) 6 Wa-ASTs through a sex peptide-binding receptor, and PISCF-ASTs through somatostatin-like receptors. These receptors are conserved through evolutionary time and are being identified in numerous invertebrates by way of genome projects.Key words: allatostatin, allatotropin, juvenile hormone, muscle contraction, neuropeptides, neuropeptide receptors, insects, crustaceans, arthropods.Résumé : Trois familles différentes de peptides portent le nom d'« allatostatines » (AST) d'après leurs purifications initiales basées sur leur capacité d'inhiber la biosynthèse de l'hormone juvénile (JH). Elles incluent (i) une famille de peptides qui possèdent une séquence consensus C-terminale Y/FXFGL-NH 2 , (ii) une famille de peptides avec une séquence C-terminale conservée W(X) 6 W-NH 2 et (iii) une famille de peptides avec une séquence C-terminale PISCF, dont certains sont amidatées sur l'extrémité C-terminale. Chaque famille d'allatostatines possède des fonctions distinctes et additionnelles à l'inhibition de la synthèse de l'hormone juvénile. Une famille de peptides connus sous le nom d'« allatotropines » sert à stimuler la synthèse de la JH. Cette famille de peptides produit aussi des effets multiples selon l'espèce concernée. Des projets sur le génome et le peptidome découvrent de nouveaux membres de ces familles et il est clair que ces structures ne se retrouvent pas seulement chez les insectes, mais aussi chez une gamme d'invertébrés. Les récepteurs de ces neuropeptides ont été identifiés et ont été testé expérimentalement pour des liaisons spécifiques au ligand. Les AST-Y/FXFGLa exercent leur action par des récepteurs de type galanine, les AST-W(X)6Wa par un récepteur liant un peptide sexuel et les AST-PISCF par des récepteurs de type somatostatine. Ces récepteurs sont conservés au cours de l'évolution et sont présentement retrouvés chez de nombreux invertébrés dans le cadre des projets de génome.

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Interaction between Manduca sexta allatotropin and Manduca sexta allatostatin in the fall armyworm Spodoptera frugiperda

Cited by 63 publications

References 38 publications

Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedesallatotropin

Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedesallatotropin

Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus

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