Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis

Recent advances in the study of biomolecular interactions by capillary electrophoresis

“…In sharp contrast, the intercalators showed preference for GC over AT sequences, in good agreement with the conclusions of previous reports [111,124]. Using CZE and ACE, the binding constant of a 14-mer dsDNA-netropsin interaction has been determined [21,116]. marker, and a negatively charged molecule, o-toluic acid (OTA), was chosen to indicate the change of EOF ( but not the EOF itself).…”

“…The interaction between HSA and VO 21 or VO 3 2 was studied using CZE. VO 3 2 has only a weak binding affinity (6.0610 3 M 21 ) to HSA, but VO 21 (the reduced form of VO 3 2 ) binds to HSA with two strong affinities (K 1 = 1.2610 8 M 21 , K 2 = 8.5610 5 M 21 ) [93]. In ACE, the migration time of four compounds (warfarin, ibuprofen, surprofen, and flurbiprofen) was measured in the presence of normal or modified HSA (at tryptophan 214 or tyrosine 411).…”

“…Besides, Joule heat, high electric field, wall effect, and properties of the running buffer may also influence the migration of analytes to some extent [18]. In studies of molecular interactions, different CE modes, equipments, or even differences in ligand or receptor concentrations may cause quantitative discrepancies [19][20][21].…”

Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2

“…Based on the CZE/FA method, the peak height changes of receptor (or ligand) resulting from interacting with ligand (or receptor) can be used to estimate the binding constant [40,41]. Previous reports have successfully estimated the binding constants of two fast binding kinetic systems, TrpRS-tRNA [39], DNA-netropsin [42]. From this study, we demonstrated that fast on-and-off kinetic interactions could also be studied by CZE, but not only by ACE.…”

Capillary zone electrophoresis investigation of the interaction between heparin and granulocyte‐colony stimulating factor

“…CE has been used in a wide range of binding studies, employing chiral selectors to study the interaction of drug stereoisomers with other enantiomeric molecules [26], and investigating the interaction between biologically important molecules, such as lectin-carbohydrate [27], protein-ligand [28,29], drug-protein [30][31][32][33][34][35], DNA-ovalbumin [36], and DNA-drug [37]. Many recent reviews [38][39][40][41] report on the application of CE for binding studies, highlighting the advantages and some of the encountered limitations.…”