Interaction between PI3K and the VDAC2 channel tethers Ras-PI3K-positive endosomes to mitochondria and promotes endosome maturation

Satoh, Ayumi; Fujioka, Yoichiro; Kashiwagi, Sayaka; Yoshida, Aiko; Fujioka, Yoichiro; Sasajima, Hitoshi; Nanbo, Asuka; Amano, Maho; Ohba, Yusuke

doi:10.1016/j.celrep.2023.112229

Cited by 10 publications

(14 citation statements)

References 73 publications

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“…Moreover, at 15 and 25 h, the number of ingested particles was markedly higher for the FND-anti-VDAC2 group compared to bare FNDs. VDAC2 was proven to promote clathrin-independent endocytosis . This unique character might explain the higher cellular uptake of antibody-coated FNDs.…”

Section: Resultsmentioning

confidence: 95%

“…VDAC2 was proven to promote clathrin-independent endocytosis. 38 This unique character might explain the higher cellular uptake of The time taken to reach equilibrium reflected the quantity of magnetic noise (in this case free radicals) present. Higher levels of free radicals resulted in a quicker decay and a lower T1 value.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…VDAC2 was proven to promote clathrin-independent endocytosis. 38 This unique character might explain the higher cellular uptake of antibody-coated FNDs. In the FND-anti-VDAC2 group, particle aggregation was observed after prolonged incubation ( Figure 2 a).…”

Section: Resultsmentioning

confidence: 99%

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Quantum Sensing of Free Radical Generation in Mitochondria of Human Keratinocytes during UVB Exposure

Fan,

Lopez Llorens,

Perona Martinez

et al. 2024

ACS Sens.

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Ultraviolet (UV) radiation is known to cause skin issues, such as dryness, aging, and even cancer. Among UV rays, UVB stands out for its ability to trigger problems within cells, including mitochondrial dysfunction, oxidative stress, and DNA damage. Free radicals are implicated in these cellular responses, but they are challenging to measure due to their short lifetime and limited diffusion range. In our study, we used a quantum sensing technique (T1 relaxometry) involving fluorescent nanodiamonds (FNDs) that change their optical properties in response to magnetic noise. This allowed us to monitor the free radical presence in real time. To measure radicals near mitochondria, we coated FNDs with antibodies, targeting mitochondrial protein voltage-dependent anion channel 2 (anti-VDAC2). Our findings revealed a dynamic rise in radical levels on the mitochondrial membrane as cells were exposed to UVB (3 J/cm2), with a significant increase observed after 17 min.

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Section: Resultsmentioning

confidence: 95%

Section: ■ Results and Discussionmentioning

confidence: 99%

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Quantum Sensing of Free Radical Generation in Mitochondria of Human Keratinocytes during UVB Exposure

Fan,

Lopez Llorens,

Perona Martinez

et al. 2024

ACS Sens.

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“…VDAC2 was proven to promote the endosome maturation process, which regulates the endocytosis pathway. This permits the escape of FNDs from endocytosis and thus enables mitochondria targeting . Also, these particles have been characterized before, and their ability to bind to mitochondria has been demonstrated .…”

Section: Methodsmentioning

confidence: 99%

Quantum Sensing of Free Radical Generation in Mitochondria of Single Heart Muscle Cells during Hypoxia and Reoxygenation

Fan,

Gao,

Zhang

et al. 2024

ACS Nano

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Cells are damaged during hypoxia (blood supply deprivation) and reoxygenation (oxygen return). This damage occurs in conditions such as cardiovascular diseases, cancer, and organ transplantation, potentially harming the tissue and organs. The role of free radicals in cellular metabolic reprogramming under hypoxia is under debate, but their measurement is challenging due to their short lifespan and limited diffusion range. In this study, we employed a quantum sensing technique to measure the real-time production of free radicals at the subcellular level. We utilize fluorescent nanodiamonds (FNDs) that exhibit changes in their optical properties based on the surrounding magnetic noise. This way, we were able to detect the presence of free radicals. To specifically monitor radical generation near mitochondria, we coated the FNDs with an antibody targeting voltage-dependent anion channel 2 (anti-VDAC2), which is located in the outer membrane of mitochondria. We observed a significant increase in the radical load on the mitochondrial membrane when cells were exposed to hypoxia. Subsequently, during reoxygenation, the levels of radicals gradually decreased back to the normoxia state. Overall, by applying a quantum sensing technique, the connections among hypoxia, free radicals, and the cellular redox status has been revealed.

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“…Mitochondrial dynamics, mitochondrial fusion and fission, endoplasmic reticulum marks fusion and fission sites, calcium transfer, autophagy, iron homeostasis, lipid transport [2,6,20,23,72,73] Lipid droplets Lipid droplet expansion, fatty acid oxidation [74] Peroxisomes Coordinate fatty acid metabolism, ROS scavenging [33] Endosomes Endosome maturation, acidification of early endosomes [36] Iron transfer [75] Mitochondrial mRNA translation in axon [38] Lysosomes Mitochondrial calcium transfer, lipid transport [40] Actin cytoskeleton Mitochondrial dynamic, mitochondrial shape, mitochondrial positioning [1,7]…”

Section: Endoplasmic Reticulummentioning

confidence: 99%

Mechanisms of Modulation of Mitochondrial Architecture

Muñoz,

Basei,

Rojas

et al. 2023

Biomolecules

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Mitochondrial network architecture plays a critical role in cellular physiology. Indeed, alterations in the shape of mitochondria upon exposure to cellular stress can cause the dysfunction of these organelles. In this scenario, mitochondrial dynamics proteins and the phospholipid composition of the mitochondrial membrane are key for fine-tuning the modulation of mitochondrial architecture. In addition, several factors including post-translational modifications such as the phosphorylation, acetylation, SUMOylation, and o-GlcNAcylation of mitochondrial dynamics proteins contribute to shaping the plasticity of this architecture. In this regard, several studies have evidenced that, upon metabolic stress, mitochondrial dynamics proteins are post-translationally modified, leading to the alteration of mitochondrial architecture. Interestingly, several proteins that sustain the mitochondrial lipid composition also modulate mitochondrial morphology and organelle communication. In this context, pharmacological studies have revealed that the modulation of mitochondrial shape and function emerges as a potential therapeutic strategy for metabolic diseases. Here, we review the factors that modulate mitochondrial architecture.

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Interaction between PI3K and the VDAC2 channel tethers Ras-PI3K-positive endosomes to mitochondria and promotes endosome maturation

Cited by 10 publications

References 73 publications

Quantum Sensing of Free Radical Generation in Mitochondria of Human Keratinocytes during UVB Exposure

Quantum Sensing of Free Radical Generation in Mitochondria of Human Keratinocytes during UVB Exposure

Quantum Sensing of Free Radical Generation in Mitochondria of Single Heart Muscle Cells during Hypoxia and Reoxygenation

Mechanisms of Modulation of Mitochondrial Architecture

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