Interaction between Saccharomyces cerevisiae and Lactobacillus fermentum during co-culture fermentation

Carvalho, Rodrigo Setem; Cruz, Ianny Andrade; Américo-Pinheiro, Juliana Heloísa Pinê; Soriano, Renato Nery; Souza, Ranyere Lucena de; Bilal, Muhammad; Iqbal, Hafiz M.N.; Bharagava, Ram Naresh; Ferreira, Luiz Fernando Romanholo

doi:10.1016/j.bcab.2020.101756

Cited by 8 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The large-scale use of antibiotic can induce bacterial resistance (Carvalho et al 2020). Also, antibiotic residues such as virginiamycin can be found in distillers dried grain (DDG), from bioethanol fermentation of corn, which is utilized for animals feed (Bischoff et al 2016).…”

Section: Resultsmentioning

confidence: 99%

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Calegari¹,

Silva²,

Gomes³

et al. 2021

Preprint

View full text Add to dashboard Cite

The presence of microbial contamination in the wort during the fermentation process results in damages of billions of dollars per year all around the world and promotes the industry dependence of chemicals and antibiotics to control the contamination. For these reasons, this study aimed to use the electron beam to sterilise wort from sugarcane molasses and investigate its bioethanol fermentation. Five treatments (T0 – T4) were carried out using ionizing doses of radiation through the electron accelerator: (0, 10, 20, 40, and 80 kGy) and a positive control (T5-steam by autoclave) were performed. It was evaluated total mesophiles, total bacteria, sugars, phenolics, flavonoids, 5-hidroximetylfurfural, and Furfural. After the irradiation process, it was conducted an alcoholic fermentation assay using baker’s yeast Saccharomyces cerevisiae. It was not observed inversion of sugars and formation of the inhibitory by-products flavonoids, furfural, and 5-hidroximetylfurfural, with exception of the phenolic compounds. Only T4 and T5 were able to sterilise the wort. However, T3 was able to eliminate > 99.99% of the microorganisms. In the fermentation T2 promoted the best ethanol yield and productivity among the irradiated treatments, evidencing the possibility of electron beam use in the wort treatment prior to fermentation which may allow a reduction in losses caused by microbial contamination, besides the possibility of promoting fermentation yield and productivity increase. The operation cost for applying the e-beam with a dose of 20 kGy in a standard ethanol plant was estimated at US$ 0.014 per m3 of wort.

show abstract

Section: Resultsmentioning

confidence: 99%

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Calegari¹,

Silva²,

Gomes³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The substrates, such as animal fats (bovine tallow and lard), greases (trap grease and float grease), used cooking oils, and vegetable oils (rapeseed, cotton seed, palm, soybean, corn, jatropha, peanut, sunflower, and canola), are used for biodiesel production by transesterification. Transesterification can be either catalytic or non-catalytic, and hence, the production of biodiesel by using enzymes has become popular now due to its low energy consumption and mild reaction conditions (Vilar et al, 2018;Pérez et al, 2019;Carvalho et al, 2020). Transesterification reaction requires a small chain alcohol (methanol, ethanol, and propanol), a catalyst (acid, base, and catalyst), and either vegetable oil or animal fats (Almeida et al, 2021).…”

Section: In Biodiesel Productionmentioning

confidence: 99%

“…The lipase-mediated bioremediation of wastewater is a novel, robust, and extensively used waste management technique in lipase biotechnology. Lipases have applications in activated sludge and other aerobic waste processes where thin layers of fats are continuously removed from the surface of aerated tanks to maintain the oxygen supply; this skimmed fast rich liquid is digested by the lipases (Cruz et al, 2020). Fungal sp.…”

Section: In the Bioremediation Of Wastewatermentioning

confidence: 99%

Industrial applications of fungal lipases: a review

Kumar¹,

Verma²,

Dubey³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Fungal lipases (triacylglycerol acyl hydrolases EC 3.1.1.3) are significant industrial enzymes and have several applications in a number of industries and fields. Fungal lipases are found in several species of fungi and yeast. These enzymes are carboxylic acid esterases, categorized under the serine hydrolase family, and do not require any cofactor during the catalyzing of the reactions. It was also noticed that processes including the extraction and purification of lipases from fungi are comparatively easier and cheaper than other sources of lipases. In addition, fungal lipases have been classified into three chief classes, namely, GX, GGGX, and Y. Fungal lipases have applications not only in the hydrolysis of fats and oils (triglycerides) but are also involved in synthetic reactions such as esterification, acidolysis, alcoholysis, interesterification, and aminolysis. The production and activity of fungal lipases are highly affected by the carbon source, nitrogen source, temperature, pH, metal ions, surfactants, and moisture content. Therefore, fungal lipases have several industrial and biotechnological applications in many fields such as biodiesel production, ester synthesis, production of biodegradable biopolymers, formulations of cosmetics and personal care products, detergent manufacturing, degreasing of leather, pulp and paper production, textile industry, biosensor development, and drug formulations and as a diagnostic tool in the medical sector, biodegradation of esters, and bioremediation of wastewater. The immobilization of fungal lipases onto different carriers also helps in improving the catalytic activities and efficiencies of lipases by increasing thermal and ionic stability (in organic solvents, high pH, and temperature), being easy to recycle, and inducing the volume-specific loading of the enzyme onto the support, and thus, these features have proved to be appropriate for use as biocatalysts in different sectors.

show abstract

“…The large-scale use of antibiotics can induce bacterial resistance (Carvalho et al, 2020). Besides, antibiotic residues, such as virginiamycin, can be found in distiller's dried grain (DDG) from bioethanol fermentation of corn, which is used as animal feed (Bischoff et al, 2016).…”

Section: Wort Irradiationmentioning

confidence: 99%

Wort disinfection treatment with electron beam for bioethanol production

Calegari

Silva

et al. 2023

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Microbial contamination of the wort during the fermentation process causes significant losses in ethanol production worldwide and creates a dependence of the industry on chemicals and antibiotics to control contamination. Therefore, this study used electron beam (e-beam) to disinfect wort from sugarcane (Saccharum officinarum L.) molasses and investigate the bioethanol fermentation. Four treatments (T0 -T3) were carried out using ionizing doses of radiation through the electron accelerator: 0 (control), 10, 20, and 40 kGy. Total mesophiles, total bacteria, sucrose, glucose, fructose, phenolics, flavonoids, hydroxymethylfurfural (5-HMF), and Furfural were measured. An alcoholic fermentation assay was performed after the irradiation process. The irradiated treatments showed no inversion of sugars and formation of the inhibitory by-products flavonoids, furfural and 5-HMF, except for the phenolic compounds. The lower dose tested (10 kGy) reduced more than 99.9 % of the total mesophiles and more than 99.99 % of the total bacteria in the substrate. In the fermentation, the irradiated worts presented similar (p > 0.05) yields (92, 93, and 94 %) and ethanol productivity levels (0.89, 0.88, and 0.87 g L -1 h -1 , for T1, T2, and T3 respectively). However, all treatments presented higher yields and productivity (p < 0.05) when compared to the control (88 % and 0.85 g L -1 h -1 ), highlighting the possible use of e-beam in wort fermentation at a lower dose (10 kGy). This allows reduction in losses caused by microbial contamination, besides increasing fermentation yield and productivity with lower energy consumption.

show abstract

Interaction between Saccharomyces cerevisiae and Lactobacillus fermentum during co-culture fermentation

Cited by 8 publications

References 36 publications

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Sterilisation of Wort from Sugarcane Molasses by Electron Beam for Bioethanol Production

Industrial applications of fungal lipases: a review

Wort disinfection treatment with electron beam for bioethanol production

Contact Info

Product

Resources

About