Interaction of Bioactive Components Caffeoylquinic Acid Derivatives in Chinese Medicines with Bovine Serum Albumin

Binding of naturally occurring hydroxycinnamic acids to bovine serum albumin

“…This was perhaps caused by steric hindrance in molecule of sinapic acid. The obtained bimolecular quenching constant for chlorogenic acid-BSA system is in good agreement with data found in literature [19]. However, published data are inconsistent and even in one case no quenching by chlorogenic acid was observed [20].…”

“…The value of K b reported by Tang et al [19] for chlorogenic acid-BSA system using fluorescence quenching method is lower than value obtained in performed experiments, but the authors used higher concentrations of chlorogenic acid and different experimental conditions. Moreover, Rawel et al [20,21] reported that chlorogenic acid does not quench Trp fluorescence in BSA and determined its binding constant by Hummel-Dreyer/size exclusion chromatography which showed significantly lower value of K b in comparison with results presented by Tang et al [19]. Non-covalent interactions of chlorogenic acid with BSA have been studied by Prigent et al [33], who reported that these interactions decrease with the increasing temperature while pH and ionic strength had no pronounced effect.…”

“…Spectroscopic techniques, such as ultraviolet-visible (UV-VIS) absorption spectroscopy [17], fluorescence spectroscopy [1,6], circular dichroism [18], and attenuated total reflectance-Fourier transform infrared spectroscopy [19] are commonly used tools to observe conformational changes in structure of proteins because of non-destructive measurements of substances in low concentration under physiological conditions, high sensitivity, rapidity and ease of implementation. Fluorescence spectroscopy is widely used to study mechanism of the binding between drugs and plasma proteins [6].…”

“…Several spectroscopic studies on the interaction between bovine serum albumin and cinnamic acid [1], ferulic acid [20,21], chlorogenic acid [19,22] or various flavonoids [18,19,24] have been published. Also several studies dealing with the interaction of human serum albumin (HSA) with derivatives of cinnamic acid or flavonoids have been carried out [2,3,17,[25][26][27][28][29].…”

On enhancing the solubility of curcumin by microencapsulation in whey protein isolate via spray drying

“…Fluorescence spectroscopy is a useful approach to study the interactions between ligands and proteins, since the fluorophore is sensitive to the polarity of its surrounding environment (Liang et al, 2007;Tang et al, 2008). The fluorescence emission spectra of curcumin solutions in the presence of different amounts of WPI were displayed in Fig.…”