Interaction of HaNPVs with two novel insecticides against Helicoverpa armigera Hubner (Noctuidae: Lepidoptera)

Abstract

Nucleopolyhedrosis viruses can be utilized for effective management of agriculture pests. Their efficacy can be increased if they are mixed with certain insecticides. In the current study, HaNPV was mixed with two insecticides: spinetoram and emamectin benzoate in various combinations and applied to larvae of H. armigera in laboratory conditions. There were a total of 15 combinations of HaNPV with each of the two insecticides in addition to five doses of HaNPV and three doses of insecticides alone. The synergistic and antagonistic effects of combinations were explored. The results revealed that there was synergistic effect of HaNPV @ 0.5 × 10 9 PIB/ml × Spinetoram @ 40, 20, 10 ml/100 L of water. In case of emamectin benzoate, synergistic effects were recorded at 1 × 10 9 PIB/ml HaNPV × emamectin benzoate @ 100 ml/100 L of water. However, 0.5 × 10 9 PIB/ml HaNPV has synergistic effects with all three doses of emamectin benzoate. The results suggested that HaNPV can be used in combination with spinetoram and emamectin benzoate for the management of resistant population of H. armigera .

Sub-lethal doses of Nucleopolyhedrosis Virus and synthetic ınsecticides alter the biological parameters of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

“…The sub-lethal doses (LC 25 ) of HaNPV (0.32×10 9 ) spinetoram (192.39 mg/l), emamectin benzoate (834.32 mg/l), spinetoram+HaNPV (0.25×10 9 PIB/ml, 129.51mg/l), emamectin benzoate+HaNPV (0.25 PIB/ml ×10 9 , 890.09 mg/l) and untreated control were used based on previous published study of Abid et al [ 33 ]. For this purpose, 200 second instar larvae of each of emamectin benzoate selected and un-selected population were exposed to sub-lethal doses of each treatment separately in petridishes containing treated diet.…”

Bioefficacy of Emamectin Benzoate against American Bollworm, Helicoverpa armigera Under Field Conditions. الفعالية الحيوية لمبيد إيمامکتين بنزوات ضد دودة اللوز الأمريکية بمحصول القطن تحت الظروف الحقلية

“…Chemical control is a rapid and consistent manner for pest management, but there is an urgent need to substitute older conventional insecticides with new ones to protect the environment. Consequently, finding alternative methods and compounds are recommended for the sustainable management of H. armigera without causing damage to the non-target organisms and the environment (Abid et al, 2020b). Emamectin benzoate 5 SG (EB) is one of the bioinsecticides confirmed to be greatly active against lepidopteran insects (Moustafa et al, 2018;Temiz, 2020;Liu, 2021).…”

“…EB has mainly consisted of avermectin B1a (90%) and avermectin B1b (10%) as mentioned by Mushtaq et al (1997). It is promoted in various formulations, but there is a lack of data establishing the relative efficacy of these formulations against lepidopteran insects (Abid et al, 2020b). At the same time, to overcome the previously mentioned problems, identification of effective doses with higher insecticidal property and lower mammalian toxicity that fits well in the integrated pest management concept is important currently.…”

“…It has both stomach and contact actions against the tested pest. The HaNPV is mixed with spinetoram and EB at lower doses of HaNPV for controlling H. armigera (Abid et al, 2020b).…”