Interaction of Interceed oxidized regenerated cellulose with macrophages: A potential mechanism by which Interceed may prevent adhesions

Reddy, Sujatha; Santanam, Nalini; Reddy, PH; Rock, John A.; Murphy, Ana A.; Parthasarathy, S.

doi:10.1016/s0002-9378(97)70070-x

Cited by 37 publications

(27 citation statements)

References 17 publications

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“…15 However, the specific mechanism of absorption of this cellulosic material has not been well understood yet, but macrophage processing presumably plays a role. 16,17 Although such nonmicrobial forms of cellulose may be swollen and then dissolved when exposed to body fluids, conversion of the cellulose into…”

Section: Introductionmentioning

confidence: 99%

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

Catchmark

2011

J Biomed Mater Res

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Cellulose biodegradation resulting from enzymolysis generally occurs in nature rather than in the human body because of the absence of cellulose degrading enzymes. In order to achieve in-vivo degradation in human body for in-vivo tissue regeneration applications, we developed a bioaborbable bacterial cellulose (BBC) material, which integrates one or more cellulose degrading enzymes (cellulases), and demonstrated its degradability in vitro using buffers with pH values relevant to wound environments. We introduced a double lyophilizing process to retain the microstructure of the bacterial cellulose as well as the activity of embedded enzymes allowing for long-term storage of the material, which only requires hydration before use. Enzymes and their combinations have been examined to optimize the in-vitro degradation of the BBC material. In-vitro studies revealed that acidic cellulases from Trichoderma viride showed reasonable activity for pH values ranging from 4.5 to 6.0. A commercial cellulase (cellulase-5000) did not show good activity at pH 7.4, but its degrading ability increased when used in conjunction with a β-glucosidase from Bacillus subtilis or a β-glucosidase from Trichoderma sp. Given the harmless glucose product of the enzymatic degradation of cellulose, the BBC material may be ideal for many wound care and tissue engineering applications for the bioabsorbable purpose.

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Section: Introductionmentioning

confidence: 99%

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

Catchmark

2011

J Biomed Mater Res

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“…It helps to prevent postoperative adhesions by protecting and separating the surfaces of denuded peritoneum where adhesions are likely to form, and it dissolves as healing occurs. ORC increases the tissue plasminogen activator (tPA)/plasminogen activator inhibitor (PAI) 1 ratio in fibroblasts isolated from adhesion tissues (10) and reduces the inflammatory response by acting on macrophages, another potential mechanism to decrease adhesion formation (11). One study reported that 55%-97% of women undergoing pelvic surgery form adhesions; several investigators reported that adhesions after myomectomy by laparotomy occurred in all patients (12).…”

mentioning

confidence: 99%

Adhesion formation after intracapsular myomectomy with or without adhesion barrier

Tinelli

Malvasi

Guido

et al. 2011

Fertility and Sterility

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“…In 1997 Reddy et al (3) reported that solubilized Interceed ® decreased the inflammatory response of macrophages. Interceed ® is taken up by macrophage scavenger receptors, resulting in a decreased binding of usual ligands, such as lipopolysaccharide.…”

Section: Discussionmentioning

confidence: 99%

“…However, recent work has questioned whether there may be an additional biological effect of Interceed ® that mediates its effectiveness as an adhesion barrier (3,4). These effects could come from the tissue upon which it is in contact, namely, mesothelial cells of normal peritoneum and fibroblasts of the underlying tissue at sites previously occupied by normal peritoneum and adhesions.…”

mentioning

confidence: 99%

Effect of oxidized regenerated cellulose (Interceed®) on the expression of tissue plasminogen activator and plasminogen activator inhibitor-1 in human peritoneal fibroblasts and mesothelial cells

Gago

Saed

El-Hammady

et al. 2006

Fertility and Sterility

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Interaction of Interceed oxidized regenerated cellulose with macrophages: A potential mechanism by which Interceed may prevent adhesions

Cited by 37 publications

References 17 publications

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

Adhesion formation after intracapsular myomectomy with or without adhesion barrier

Effect of oxidized regenerated cellulose (Interceed®) on the expression of tissue plasminogen activator and plasminogen activator inhibitor-1 in human peritoneal fibroblasts and mesothelial cells

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