Interaction of TiO₂ nanoparticles with lung fluid proteins and the resulting macrophage inflammatory response

Abstract

Inhalation is a major exposure route to nanoparticles. Following inhalation, nanoparticles first interact with the lung lining fluid, a complex mixture of proteins, lipids, and mucins. We measure the concentration...

House dust mite extract forms a der p 2 corona on multi-walled carbon nanotubes: implications for allergic airway disease

“…For example, titanium nanoparticles incubated with fetal bovine serum form a corona of 50 μg protein/mg of titanium dioxide nanoparticles. 32 It is possible that HDM, which is a mixture of mites, nymphs, fecal matter, and eggs, 37 results in maximum corona formation at relatively low corona concentrations values. In comparison, much of the previous work has focused on human plasma or bovine serum relevant to cell culture and biomedical applications.…”

“…Our procedure for obtaining BALF has been described previously. 32 All procedures were approved by the Duke University Institutional Animal Care and Use Committee Environmental Science: Nano Paper (IACUC) and were performed under an IACUC approved animal protocol (A053-21-03). All animal experiments were conducted in accordance with the American Association for the Accreditation of Laboratory Animal Care guidelines.…”

“…The interaction of titanium dioxide and polystyrene nanoparticles with bronchoalveolar lavage fluid (BALF) has been characterized previously. 31,32 For the titanium dioxide nanoparticles, a BALF corona led to increased expression of pro-inflammatory cytokines in macrophages. 32 To the best of our knowledge, our work described below is the first to address a combined allergen and lung fluid corona.…”

“…31,32 For the titanium dioxide nanoparticles, a BALF corona led to increased expression of pro-inflammatory cytokines in macrophages. 32 To the best of our knowledge, our work described below is the first to address a combined allergen and lung fluid corona.…”

Sources of biases in the in vitro testing of nanomaterials: the role of the biomolecular corona

“…Only a few attempts have been made so far to break tradition, considering that particular applications and cellular experiments might require a more specific biological environment, not consistently recapitulated well by the use of FBS. 15–21 In some cases, different media have been considered to mimic possible entry routes, such as lung inhalation, 22–24 ocular injection, 25 and intramuscular injection (as in the case of vaccines). 26 These alternative media have had less impact in the nanotoxicology space than they deserved.…”