Interaction studies between a 1,10-phenanthroline adduct of palladium(II) dithiocarbamate anti-tumor complex and calf thymus DNA. A synthesis spectral and in-vitro study

Mansouri‐Torshizi, Hassan; Saeidifar, Maryam; Divsalar, Adeleh; Saboury, Ali Akbar

doi:10.1016/j.saa.2010.05.029

Cited by 55 publications

(17 citation statements)

References 42 publications

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“…In the study of Mukherjee et al using Pd complexes with a hydrazone ligand, prostate cancer (PC-3) cell growth was inhibited at G2/M phase and apoptosis induced by activation of caspase-3 [46]. Metal complexes containing 1,10-phenanthroline are known to produce DNA adducts by intercalation, which inhibit replication and transcription of DNA [33]. …”

Section: Discussionmentioning

confidence: 99%

“…Thus, in this study, we report the cytotoxicity of three palladium(II) complexes with dithiocarbamate ligands and the effect of hydrocarbon chain lengths (propylene, butylene, and octylene) in the structure of these complexes on the anticancer activity compared to cisplatin. The Pd(II) complexes are expected to bind DNA by intercalation [33], a mechanism different from that of interaction of cisplatin with DNA. Metal complexes that contain 1,10-phenanthroline have been known to have effective anticancer activity and also to be intercalated between the DNA base pairs [34–36].…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Effects of Newly Synthesized Palladium(II) Complexes of Diethyldithiocarbamate on Gastrointestinal Cancer Cell Lines

Hadizadeh

Najafzadeh

Mazani

et al. 2014

Biochemistry Research International

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As a part of a drug development program to discover novel therapeutic and more effective palladium (Pd) based anticancer drugs, a series of water-soluble Pd complexes have been synthesized by interaction between [Pd (phen)(H2O)2(NO3)2] and alkylenebisdithiocarbamate(al-bis-dtc) disodium salts. This study was undertaken to examine the possible cytotoxic effect of three novel complexes (0.125–64 µg/mL) on human gastric carcinoma (AGS), esophageal squamous cell carcinoma (Kyse-30), and hepatocellular carcinoma (HepG2) cell lines. The cytotoxicity was examined using cell proliferation and acridine orange/ethidium bromide (AO/EB) assay. In order to examine the effects of new Pd(II) complexes on cell cycle status, we performed cell cycle analysis. The complexes were found to have completely lethal effects on the cell lines, and the half maximal inhibitory concentration (IC50) values obtained for the cell lines were much lower in comparison with cisplatin. We demonstrated that the three new Pd(II) complexes are able to induce G2/M phase arrest in AGS and HepG2; in addition, the Pd(II) complexes caused an S phase arrest in Kyse-30 cell line. Our results indicate that newly synthesized Pd(II) complexes may provide a novel class of chemopreventive compounds for anticancer therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Newly Synthesized Palladium(II) Complexes of Diethyldithiocarbamate on Gastrointestinal Cancer Cell Lines

Hadizadeh

Najafzadeh

Mazani

et al. 2014

Biochemistry Research International

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“…The procedure followed was similar to that reported earlier [47, 48]. These experiments were carried out separately at two temperatures of 300 K and 310 K in Tris-HCl buffer medium.…”

Section: Resultsmentioning

confidence: 98%

DNA Binding and Antitumor Activity of α-Diimineplatinum(II) and Palladium(II) Dithiocarbamate Complexes

Mansouri‐Torshizi

Saeidifar

Khosravi

et al. 2011

Bioinorganic Chemistry and Applications

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The two water-soluble designed platinum(II) complex, [Pt(Oct-dtc)(bpy)]NO3 (Oct-dtc = Octyldithiocarbamate and bpy = 2,2′ -bipyridine) and palladium(II) complex, [Pd(Oct-dtc)(bpy)]NO3, have been synthesized and characterized by elemental analyses, molar conductivity measurements, IR, 1H NMR, and electronic spectra studies. Studies of antitumor activity of these complexes against human cell tumor lines (K562) have been carried out. They show Ic50 values lower than that of cisplatin. The complexes have been investigated for their interaction with calf thymus DNA (CT-DNA) by utilizing the electronic absorption spectroscopy, fluorescence spectra, and ethidium bromide displacement and gel filtration techniques. Both of these water-soluble complexes bound cooperatively and intercalatively to the CT-DNA at very low concentrations. Several binding and thermodynamic parameters are also described.

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“…30,31 In this experiment, the sample cell was filled with 1.8 mL CT-DNA (~0.20 mM for [Pd(phen)(pip-dtc)]NO 3 and ~0.17 mM for [Pd(phen) (mor-dtc)]NO 3 complexes). In these concentrations, the absorption of CT-DNA is around 1.32 and 1.12, respectively.…”

Section: Ct-dna-denaturation Studies and Determination Of Thermodynammentioning

confidence: 99%

DNA Binding Studies and Cytotoxicity of the Novel 1,10-phenanthroline Palladium(II) Complexes of Dithiocarbamate Derivatives

Mansouri‐Torshizi

Saeidifar²,

Ghasemi³

et al. 2011

Journal of the Korean Chemical Society

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, (where phen is 1,10-Phenantroline, pip-dtc is piperidinedithiocarbamate anion and mor-dtc is morpholinedithiocarbamate anion) have been synthesized and characterized by elemental analysis, spectroscopic studies (FT-IR, 1 H NMR, UV-Vis) and conductance measurement. In these complexes, the dithiocarbamate ligands coordinate with Pd (II) center as bidentate with two sulfur atoms. These two complexes have been tested against chronic myelogenous leukemia cell line, K562. They show IC50 values less than cisplatin and thus the mode of binding of the complexes to calf thymus DNA (CT-DNA) were investigated by ultraviolet difference and fluorescence spectroscopy. They can denature DNA, exhibit cooperative binding and intercalate into DNA. Several binding and thermodynamic parameters are also described.

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Interaction studies between a 1,10-phenanthroline adduct of palladium(II) dithiocarbamate anti-tumor complex and calf thymus DNA. A synthesis spectral and in-vitro study

Cited by 55 publications

References 42 publications

Cytotoxic Effects of Newly Synthesized Palladium(II) Complexes of Diethyldithiocarbamate on Gastrointestinal Cancer Cell Lines

Cytotoxic Effects of Newly Synthesized Palladium(II) Complexes of Diethyldithiocarbamate on Gastrointestinal Cancer Cell Lines

DNA Binding and Antitumor Activity of α-Diimineplatinum(II) and Palladium(II) Dithiocarbamate Complexes

DNA Binding Studies and Cytotoxicity of the Novel 1,10-phenanthroline Palladium(II) Complexes of Dithiocarbamate Derivatives

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