Interactions among Peroxisome Proliferator Activated Receptor-γ, Insulin Signaling Pathways, and Steroidogenic Acute Regulatory Protein in Human Ovarian Cells

Seto-Young, Donna; Avtanski, Dimiter; Strizhevsky, Marina; Parikh, Grishma; Patel, Parini; Kaplun, Julia; Holcomb, Kevin; Rosenwaks, Zev; Poretsky, Leonid

doi:10.1210/jc.2006-1935

Cited by 52 publications

(38 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, inconsistency in their expression and activity has been often demonstrated by researchers. For instance, a stimulatory effect of PPARg activation on steroidogenesis can be mediated by up-regulation of steroidogenic acute regulatory (StAR) protein in human and macaque granulosa cells [44,45] or by 3b-HSD in porcine ovarian follicles [36]. An opposite effect of PPARg ligands on 3b-HSD expression and P 4 release was observed in porcine granulosa cells [37].…”

Section: Ppars and Steroidogenesismentioning

confidence: 99%

Peroxisome proliferator-activated receptors in the regulation of female reproductive functions

Bogacka

Kurzyńska

Bogacki

et al. 2015

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Peroxisome proliferator-activated receptors (PPARs) belong to a ligand-dependent nuclear receptor family. In the past decade, numerous studies have revealed the presence and significance of PPARs in the reproductive system. PPARs are expressed at different levels of hypothalamic-pituitary-gonadal (HPG) axis. They are also present in the uterus as well as in the placenta and embryonic tissues of different species. PPARs significance has been reported during the estrous/menstrual cycle and pregnancy with the gamma isoform studied most frequently. Several studies indicate that PPARs regulate proliferation of ovarian cells, tissue remodeling and steroidogenesis. In the endometrium, PPARs are engaged in the regulation of prostaglandins, steroids and cytokines synthesis. The role of PPARs in the trophoblast differentiation, maturation and invasion as well as in the embryo development has also been demonstrated. In this review, we summarize current findings concerning the role of PPARs in the regulation of reproductive functions at different levels of the HPG axis during various physiological statuses of females. In addition, the role of PPARs in the modulation of uterine functions as well as the placenta and embryo development has also been discussed.

show abstract

Section: Ppars and Steroidogenesismentioning

confidence: 99%

Peroxisome proliferator-activated receptors in the regulation of female reproductive functions

Bogacka

Kurzyńska

Bogacki

et al. 2015

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…Moreover, it is involved in adipocyte differentiation, fat deposition in adipose tissue, improving insulin sensitivity [24] and regulating ovarian steroidogenesis [25]. Polymorphisms of PPARγ gene have been strongly implicated in the etiology of IR, T2DM [11] and obesity [18].…”

Section: Introductionmentioning

confidence: 99%

Peroxisome proliferator activated receptor gamma gene variants influence susceptibility and insulin related traits in Indian women with polycystic ovary syndrome

Shaikh

Mukherjee

Shah³

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Peroxisome proliferator activated receptor gamma (PPARγ), a transcription factor involved in glucose and lipid metabolism is one of the candidate genes associated with polycystic ovary syndrome (PCOS). We investigated individual and combined associations of Pro12Ala and His447His polymorphisms of PPARγ with PCOS susceptibility and its related traits (hyperinsulinemia, hyperandrogenemia and lipid parameters) in Indian women.Method Genotyping of PPARγ polymorphisms in this casecontrol study was performed in PCOS (n=450) and agematched controls (n=300) by direct sequencing. Clinical, anthropometric, hormonal and metabolic parameters were estimated in 275 women with PCOS and 169 controls. Chisquare test was used to compare the categorical data while regression analysis was used to evaluate association of genotypes with PCOS as well as its related phenotypes. Results The frequencies of CC and CG+GG genotypes of Pro12Ala (χ 2 =15.3, p<0.0001) and CC and CT+TT genotypes of His447His (χ 2 =12.7, p=0.0004) polymorphisms were significantly different between PCOS and controls. Logistic regression analysis revealed a significant association of PCOS with Pro12Ala but not the His447His polymorphism. Carriers of variant genotypes at both PPARγ loci showed significantly reduced 2 h glucose levels while carriers of variant His447His genotype showed lower fasting insulin and HOMA-IR levels in PCOS women. Conclusions Pro12Ala polymorphism of PPARγ showed significant association with decreased PCOS susceptibility. Both polymorphisms influenced insulin related traits (2 h glucose, fasting insulin and HOMA-IR) and improved glucose metabolism in these women. This is the first report to establish that variations in PPARγ gene influence the insulin resistance pathophysiology in Indian women with PCOS.

show abstract

“…Such a relationship has been observed in ovarian cells in different species, but the results are contradictory. For instance, PPARγ ligands enhanced P 4 release by porcine theca cells (Schoppee et al 2002) and mixed human ovarian (stroma, theca, and granulosa) cells (Seto-Young et al 2007), but inhibited hormone secretion by porcine and hu- Table 2. Effect of PPAR ligands on 17-β estradiol release by the endometrium of gilts on days 10-12 (n = 4) and 14-16 (n = 4) of the estrous cycle and days 10-12 (n = 4) and 14-16 (n = 4) of pregnancy (Gasic et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The procedure for the collection and incubation of endometrial tissue was described previously (Bogacka et al 2013b (Lovekamp et al 2001;Schoppee et al 2002;Seto-Young et al 2007). The tested compounds were dissolved in a total volume of 20 μl dimethyl sulfoxide (DMSO) (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Peroxisome proliferator activated receptor ligands affect porcine endometrial steroids production during the estrous cycle and early pregnancy: an in vitro study

Kurzyńska¹,

Bogacki²,

Chojnowska³

et al. 2016

CZECH J ANIM SCI

View full text Add to dashboard Cite

AbstrAct:In the present study, we investigated the effect of PPAR ligands on progesterone (P 4 ) and 17β-estradiol (E 2 ) secretion, as well as 3β-hydroxysteroid dehydrogenase/Δ(5)-Δ(4) isomerase (3β-HSD) mRNA expression, in porcine endometrial slices collected on days 10-12 and 14-16 of the estrous cycle or early pregnancy. The explants were incubated in vitro for 6 h in the presence of PPARα ligands -WY-14643 (agonist) and MK 886 (antagonist); PPARβ ligands -L-165041 (agonist) and GW 9662 (antagonist); PPARγ ligands -15d-prostaglandin J 2 and rosiglitazone (agonists) and T0070907 (antagonist). During the estrous cycle, all PPAR ligands inhibited P 4 secretion during the mid-luteal phase (days 10-12). During early pregnancy, a stimulatory effect of PPARα agonist was observed during maternal recognition of pregnancy (days 10-12), while an inhibitory effect was observed at the beginning of implantation (days 14-16). PPAR ligands inhibited the expression of 3β-HSD mRNA on days 14-16 of the estrous cycle (β and γ isoforms) or pregnancy (α, β, γ isoforms) but did not affect gene expression on days 10-12 of the estrous cycle or early pregnancy. An inhibitory effect of PPARα, PPARγ, and PPARβ on E 2 secretion was observed during maternal recognition of pregnancy, but a stimulatory effect was observed during mid-(γ isoform) or late-luteal (β isoform) phases of the estrous cycle. Our study indicates, for the first time, that PPARs are engaged in P 4 and E 2 production in porcine endometrium. It is possible that the diverse receptivity of endometrial tissue to the PPAR ligands can be associated with the reproductive status of gilts.

show abstract

Interactions among Peroxisome Proliferator Activated Receptor-γ, Insulin Signaling Pathways, and Steroidogenic Acute Regulatory Protein in Human Ovarian Cells

Cited by 52 publications

References 37 publications

Peroxisome proliferator-activated receptors in the regulation of female reproductive functions

Peroxisome proliferator-activated receptors in the regulation of female reproductive functions

Peroxisome proliferator activated receptor gamma gene variants influence susceptibility and insulin related traits in Indian women with polycystic ovary syndrome

Peroxisome proliferator activated receptor ligands affect porcine endometrial steroids production during the estrous cycle and early pregnancy: an in vitro study

Contact Info

Product

Resources

About