A field experiment was conducted in Khulna district to evaluate the growth and nutrient composition on stem Amaranth by irrigating with different water sources. Water that has been used as treatment are saline water, T1 and treated water, T2 (water from a project called Managed Aquifer Recharge (MAR)). A field condition was also included, T0 (control). All the growth parameters of the crop were significantly improved for treated water. Whereas, N, P, and K uptake (3.54, 39.56 and 564.42 kg/ha, respectively) were increased and S was decreased (48.76 kg/ha) for treated water (T2) compared to control (T0). Carbohydrate and total dietary fiber (TDF) is increased (6.09 and 6.87 g/100g) while protein, fat, ash and energy in the leaf of stem Amaranth is decreased (3.16, 0.46, 3.26 g/100g and 54.94 Kcal/100g respectively) by irrigating with T2 water. Moreover, carbohydrate content is increased (3.55 g/100 g) in treated water (T2) but protein, fat, ash, TDF and energy in the stem of stem Amaranth is decreased. EC, pH, Na, K, Ca and Mg of the soil were decreased markedly by applying treated water (T2) and other nutrients present in soil also varies. From the study, it can be concluded that the best alternate source of water for irrigation is MAR water (T2) which can improve plant nutrient content and uptake and soil nutrient status.Bangladesh J. Sci. Res. 28(1): 43-50, June-2015