Interactions between Human Antibodies and Synthetic Conformational Peptide Epitopes: Innovative Approach for Electrochemical Detection of Biomarkers of Multiple Sclerosis at Platinum Electrodes

Bellagha-Chenchah, Wided; Sella, C.; Real‐Fernández, Feliciana; Peroni, Elisa; Lolli, Francesco; Amatore, Christian; Thouin, Laurent; Papini, Anna Maria

doi:10.1016/j.electacta.2015.07.158

Cited by 12 publications

(9 citation statements)

References 40 publications

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“…Rheumatoid arthritis (RA) is another common AD with a prevalence of about 0.5–1%. The main symptom in RA is joint pain and deformation due to synovial chronic inflammation that leads to joint destructions and disability [61]. Clinical remission is possible in early RA; however, it is not achievable in all patients, since RA is a rather heterogeneous disease [62].…”

Section: Biosensors For Ads Based On Antibodies and Antigensmentioning

confidence: 99%

“…Furthermore, after labeling CSF114 with a ferrocenyl moiety, the peptide was immobilized on a platinum electrode, without any previous surface modification. The developed biosensor was characterized using CV, the interactions between Fc-CSF114(Glc) and autoantibodies being characterized by a shift of the oxidation potential with several tens of millivolts towards more positive values using autoantibodies concentrations higher than 0.1 mg mL −1 [61].…”

Section: Biosensors For Ads Based On Peptidesmentioning

confidence: 99%

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Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

et al. 2019

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An important class of biosensors is immunosensors, affinity biosensors that are based on the specific interaction between antibodies and antigens. They are classified in four classes based on the type of employed transducer: electrochemical, optical, microgravimetric, and thermometric and depending on the type of recognition elements, antibodies, aptamers, microRNAs and recently peptides are integrating parts. Those analytical devices are able to detect peptides, antibodies and proteins in various sample matrices, without many steps of sample pretreatment. Their high sensitivity, low cost and the easy integration in point of care devices assuring portability are attracting features that justify the increasing interest in their development. The use of nanomaterials, simultaneous multianalyte detection and integration on platforms to form point-of-care devices are promising tools that can be used in clinical analysis for early diagnosis and therapy monitoring in several pathologies. Taking into account the growing incidence of autoimmune disease and the importance of early diagnosis, electrochemical biosensors could represent a viable alternative to currently used diagnosis methods. Some relevant examples of electrochemical assays for autoimmune disease diagnosis developed in the last several years based on antigens, antibodies and peptides as receptors were gathered and will be discussed further.

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Section: Biosensors For Ads Based On Antibodies and Antigensmentioning

confidence: 99%

Section: Biosensors For Ads Based On Peptidesmentioning

confidence: 99%

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

et al. 2019

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“…Compared to microfluidic generators described above, the advantage will undoubtedly be the use of a unique microchannel, allowing rapid formation of concentration gradients while preventing issues related to multiple and parallel microchannels. In this context, electrochemical techniques can be exploited in microfluidic platforms not only for passive sensing [37][38][39][40][41][42][43][44][45] but also for active control of microenvironments. [46][47][48] The potential of electrochemistry in exerting precise modulations of concentration in constrained volumes has been successfully demonstrated for example to control dynamically the pH 46,47 or the on-demand oxygen generation.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Generation of Steady-State Linear Concentration Gradients within Microfluidic Channels Perpendicular to the Flow Field

2020

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…Electrochemical systems are also ideal for the development of point-of-care devices due to their simplicity, portability, rapidness, cost-effectiveness and sensitivity with the capability of applying various transduction methods [14]. Detection of target biomarkers directly using human sera samples can be achieved using electrochemical detection method [17,18].…”

Section: Introductionmentioning

confidence: 99%

Recent Developments of Electrochemical and Optical Biosensors for Antibody Detection

Wang

et al. 2019

IJMS

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Detection of biomarkers has raised much interest recently due to the need for disease diagnosis and personalized medicine in future point-of-care systems. Among various biomarkers, antibodies are an important type of detection target due to their potential for indicating disease progression stage and the efficiency of therapeutic antibody drug treatment. In this review, electrochemical and optical detection of antibodies are discussed. Specifically, creating a non-label and reagent-free sensing platform and construction of an anti-fouling electrochemical surface for electrochemical detection are suggested. For optical transduction, a rapid and programmable platform for antibody detection using a DNA-based beacon is suggested as well as the use of bioluminescence resonance energy transfer (BRET) switch for low cost antibody detection. These sensing strategies have demonstrated their potential for resolving current challenges in antibody detection such as high selectivity, low operation cost, simple detection procedures, rapid detection, and low-fouling detection. This review provides a general update for recent developments in antibody detection strategies and potential solutions for future clinical point-of-care systems.

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Interactions between Human Antibodies and Synthetic Conformational Peptide Epitopes: Innovative Approach for Electrochemical Detection of Biomarkers of Multiple Sclerosis at Platinum Electrodes

Cited by 12 publications

References 40 publications

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases

Electrochemical Generation of Steady-State Linear Concentration Gradients within Microfluidic Channels Perpendicular to the Flow Field

Recent Developments of Electrochemical and Optical Biosensors for Antibody Detection

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