Interactions between membrane sterols and phospholipids in model mammalian and fungi cellular membranes — A Langmuir monolayer study

Miñones, J.; País, Silvia Marina; Conde, O.; Dynarowicz-Łątka, Patrycja

doi:10.1016/j.bpc.2008.11.011

Cited by 73 publications

(63 citation statements)

References 38 publications

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“…The shape of π/ A isotherm registered for pure DPPC monolayer on the water subphase is very similar to that recorded on aqueous DS40 or Ca 2+ solutions, with a characteristic plateau at 5 mN m −1 , which have been ascribed to a phase transition between liquid-expanded and liquid-condensed (LE–LC) states (Miñones et al 2009). This plateau is visible as a minimum on C s −1 versus π plots for pure DPPC monolayer formed both on water, water-containing Ca 2+ , and aqueous DS40 solution (Fig.…”

Section: Resultssupporting

confidence: 70%

“…Although the behavior of the above membrane lipids and their mixtures have already been studied in detail (Cadena-Nava et al 2006; Sabatini et al 2008; Miñones et al 2009), we present the π/ A isotherms and BAM images recorded on water subphase for the purpose of comparison with the results obtained for DS40 and/or Ca 2+ -containing subphases.…”

Section: Resultsmentioning

confidence: 99%

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The Effect of Dextran Sulfate—as Model Glycosaminoglycan Analogue—on Membrane Lipids: DPPC, Cholesterol, and DPPC–Cholesterol Mixture. The Monolayer Study

et al. 2018

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Glycosaminoglycans (GAGs) are essential components of the extracellular matrices (ECMs) located on the outer surface of cellular membranes. They belong to the group of polysaccharides involved in diverse biological processes acting on the surface and across natural lipid membranes. Recently, particular attention has been focused on possible role of GAGs in the amyloid deposits. The amyloid formation is related to a disorder in protein folding, causing that soluble—in normal conditions—peptides become deposited extracellularly as insoluble fibrils, impairing tissue structure and its function. One of the hypothesis holds that GAGs may inhibit amyloid formation by interacting with the lipid membrane by blocking the accumulation of protein aggregates on the membrane surface. Although the biophysical properties of GAGs are described rather well, little is known about the nature of association between these polysaccharides and components of natural cell membranes. Therefore, a study of GAGs influence on membrane lipids is of particular importance. The aim of the present work is to get insight into the effect of hydrophilic dextran sulfate (DS)—that can be considered as GAG analogue—on membrane lipids organization. This study was based on examining interactions between DS sodium salt of molecular weight equal to about 40 kDa (DS40), dissolved in water subphase, and a model membrane, mimicked as Langmuir monolayer, formed by representative natural membrane lipids: cholesterol and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as well as their mixtures. Due to the fact that calcium ions in excess may accumulate in the lipid membrane, attracting high molecular weight molecules to their surface, the influence of calcium ions present in the subphase on the DS40 activity has also been examined. It has been found that negatively charged DS, forming a sublayer underneath the monolayer, barely interacts with membrane lipids; however, in the presence of calcium ions the electrostatic interactions between DS40 and lipid membrane are significantly enhanced, leading to the formation of network-like crystalline structures at the surface of model membrane, which can prevent incorporation and interaction with other extracellular molecules, e.g., proteins.

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Section: Resultssupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

The Effect of Dextran Sulfate—as Model Glycosaminoglycan Analogue—on Membrane Lipids: DPPC, Cholesterol, and DPPC–Cholesterol Mixture. The Monolayer Study

et al. 2018

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“…For simplicity, it was assumed that d remains constant in the liquid condensed phase with a value of 2.48 nm, as determined from previous BAM experiments [34]. Reflectivity values were related to the gray levels of the camera with calibrations curves as has been previously described [35,36]. Gray levels were averaged for horizontal lines at three separate pixels to determine an overall average gray level for each cropped BAM image.…”

Section: Brewster Angle Microscopy (Bam)mentioning

confidence: 99%

Effect of cation enrichment on dipalmitoylphosphatidylcholine (DPPC) monolayers at the air-water interface

Adams

Casper

Allen

2016

Journal of Colloid and Interface Science

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The effect of highly concentrated salt solutions of marine-relevant cations (Na(+), K(+), Mg(2+), and Ca(2+)) on Langmuir monolayers of dipalmitoylphosphatidylcholine (DPPC) was investigated by means of surface pressure-area isotherms, Brewster angle microscopy (BAM), and infrared reflection-absorption spectroscopy (IRRAS). It was found that monovalent cations and Mg(2+) have similar phase behavior, causing DPPC monolayers to expand, while Ca(2+) induces condensation. All cations disrupted the surface morphology at high cation concentration, resulting in decreased reflectivity from the monolayer. Monolayer refractive index was calculated from BAM image intensity in the liquid condensed phase and decreased with increasing cation concentration, which suggests that orientation of the alkyl chains change. Monovalent ions increase ordering of the alkyl chains, more than divalents, yet have little interaction with the DPPC headgroup. Mg(2+) induces gauche defects in the alkyl chain and increases headgroup hydration at low lipid coverage but increases chain ordering and dehydrates the headgroup at high lipid coverage. Ca(2+) orders alkyl chains and dehydrates the phosphate moiety, independent of lipid phase. At the highest salt concentration investigated, significant narrowing of the asymmetric PO2(-) vibrational mode occurs and is attributed to considerable dehydration of the DPPC headgroup.

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“…Studies on interactions between sterols (ergosterol or cholesterol) and phospholipids (DOPC or DPPC) devoid of AmB [140] have raised an important issue, i.e. that AmB interactions with sterols or phospholipids may compete with those occurring naturally in the biomembrane (between sterols and phospholipids).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

et al. 2015

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Interactions between membrane sterols and phospholipids in model mammalian and fungi cellular membranes — A Langmuir monolayer study

Cited by 73 publications

References 38 publications

The Effect of Dextran Sulfate—as Model Glycosaminoglycan Analogue—on Membrane Lipids: DPPC, Cholesterol, and DPPC–Cholesterol Mixture. The Monolayer Study

The Effect of Dextran Sulfate—as Model Glycosaminoglycan Analogue—on Membrane Lipids: DPPC, Cholesterol, and DPPC–Cholesterol Mixture. The Monolayer Study

Effect of cation enrichment on dipalmitoylphosphatidylcholine (DPPC) monolayers at the air-water interface

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

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