Interactions between Parenteral Lipid Emulsions and Container Surfaces

Abstract

The goal of this work is to evaluate how emulsions in total nutrition admixtures are affected by the containers within which they are stored. Specifically, the study examines how the emulsion globule size distribution in different containers is related to adsorption or absorption of the lipids onto or into the container. The admixtures were prepared from a commercial lipid emulsion, 20% ClinOleic®, and the containers were either glass (borosilicate) or plastic (ethylene vinyl acetate, EVA). The large globule size distribution was monitored continuously for both containers over the course of 24 h, and the quantity of triglycerides taken up by both containers was measured by liquid chromatography. The lipid uptake by the EVA containers was also monitored by gravimetric methods. Briefly, the percent of fat globules greater than 5 micrometers (PFAT5) in EVA containers showed a 75% reduction compared to a marginal decrease of PFAT5 when in the glass container. Extraction of the lipids from the containers showed that the quantity of triglycerides associated with the EVA surfaces steadily increased with admixture exposure time, while the glass showed a significantly lower triglyceride content. Gravimetric measurements confirmed that the EVA containers gained measurable mass during exposure to the emulsion admixture.

Automated System for Kinetic Analysis of Particle Size Distributions for Pharmaceutically Relevant Systems

“…In particular, the initial 10 hours of the study show the least variation between large globule profiles represented by PFAT 5 . The decrease in PFAT 5 over time present in the resting and rocking samples has been observed previously [17] and is attributed to triglycerides being absorbed to the EVA container. While the mechanistic details are not clear, the larger oil globule droplets are thought to preferentially and irreversibly attach to the surface of the EVA container decreasing their solution concentration over time.…”

Passive anti-amyloid immunotherapy in Alzheimer's disease: What are the most promising targets?

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“…From a chemical point of view, insulin is a lipophilic molecule. Therefore, ILE may act as an insulin protectant, shielding it from a direct reaction with the plastic materials, either by forming a film and isolating the surface from the other components, or saturating the “chemical receptor points.” Gonyon et al found evidence of interaction between large fat globules of an emulsion admixture and EVA bags, producing a 75% lipid reduction compared with an only marginal decrease in glass bottles. Quantity of triglycerides adherent to EVA surfaces steadily increased with emulsion exposure time, as proven by gravimetric measurements.…”