Interactive Effect of Heavy Metals and Gibberellic Acid on Mitotic Activity and Some Metabolic Changes of Vicia faba L. Plants

Mansour, Mahmoud; Kamel, E. A.

doi:10.1508/cytologia.70.275

Cited by 28 publications

(13 citation statements)

References 12 publications

(5 reference statements)

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“…Similarly, other scientists also reported the potentiality of GA 3 in alleviating the detrimental effect of metals hence proves its beneficial role in acclimatizing metal stress on Arabidopsis and Lupin [ 47 ]. Furthermore, GA 3 treatment greatly increased protein as compared to Cd treated plants as reported earlier [ 48 ].…”

Section: Discussionsupporting

confidence: 78%

Acclimation of cadmium-induced genotoxicity and oxidative stress in mung bean seedlings by priming effect of phytohormones and proline

et al. 2021

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In this research, eight local mung bean (Vigna radiata) varieties were analyzed for their performance against two levels of CdCl2 solution (0.3 and 0.5 mM) alone and priming with gibberellic acid (GA3) (100 μM), salicylic acid (SA) (50 μM) and proline (5 mM) solution prior to Cd exposure. Mung bean seedlings were analyzed for disturbance in cytological, morphological, biochemical and enzymatic parameters under cadmium stress. For cytological studies, 48 h grown mung bean seedlings root tips were used to prepare slides and studied for percent mitotic index (MI%) and to calculate percent C-mitosis, laggard, sticky and fragmented chromosomes, pictures were captured by a Nikon camera (DS-Fi 1 Japan) attached with a microscope. One-week grown mung seedlings were studied for growth traits, malondialdehyde (MDA), protein, proline and antioxidant enzymes. ANOVA and DMR test of this research revealed that all the tested mung bean varieties and treatments were significantly different regarding mitotic index and number of chromosomal aberrations. Both the Cd treatments exhibited increased total chromosomal aberrations with different types and a maximum decrease in MI%. In pretreated samples, GA3, SA and proline serve as mitigating agents that reduce mutagenic effects of Cd in mung bean by increasing MI% and decreasing chromosomal aberrations as compared to non-pretreated samples. Both the Cd treatments showed a decrease in all growth traits. Total proteins were also found to be significantly reduced in a dose-dependent manner in all genotypes. Cd treatment increased the activities of all antioxidant enzymes tested. Cd caused oxidative damage as indicated by elevated levels of MDA content in treated samples in comparison to control. Proline content levels were also high in Cd treated seedlings indicating stress. Results demonstrated that pretreatment with phytohormones and proline before Cd were found to improve all morphological parameters, by altering antioxidant enzymes activities along with a decrease in MDA and proline contents as well. It was further noticed that the performance of GA3 was better at 0.3 mM Cd treatment while SA was found to be a good mitigating agent at 0.5 mM Cd stress in all tested mung bean varieties. This research concluded less deleterious effects of Cd on AZRI-2006 while more sensitivity to NM-51 towards Cd. Priming with phytohormones and proline is a user-friendly, economical, and simple mitigation strategy to reduce Cd toxicity in plants and get better yield from contaminated lands.

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Section: Discussionsupporting

confidence: 78%

Acclimation of cadmium-induced genotoxicity and oxidative stress in mung bean seedlings by priming effect of phytohormones and proline

et al. 2021

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“…It was postulated that the decrease in biomass was a consequence of change in the plant water status accompanied by disturbances in biological and physiological processes such as mitotic activity, photosynthesis, respiration, and mineral nutrition. Nickel accumulation decreased water uptake or enhanced water loss, both of which may produce membrane damage (El-Enany et al, 2000;Mansour & Kamel, 2005). Our results demonstrated that maize plants cv.…”

Section: Discussionsupporting

confidence: 53%

Growth and Mineral Composition of Nickel-Stressed Plants Under Conditions of Supplementation With Excessive Amounts of Calcium and Iron

Matraszek

Hawrylak-Nowak

2010

Journal of Toxicology and Environmental Health, Part A

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This study investigated the effectiveness of excessive calcium (Ca) and iron (Fe) supplement nutrition in spinach Markiza F(1) cv. and sweet corn Zlota Karlowa cv. to alleviate nickel (Ni)-induced phytotoxicity. The following doses of the pollutant Ni were introduced: 0 (control), 40, or 60 mg Ni/kg growth medium. Two levels of calcium (Ca), 270 (basic) and 400 mg/kg (intensive), as well as two levels of iron (Fe), 10 (basic) and 20 mg/kg (intensive), respectively, were used. Intensive nutrition supplementation of Ni-stressed test plants species with Ca or Fe was beneficial as manifested by significantly increased maize shoots and roots biomass, lowered content of Ni in spinach and maize in above-ground parts, and decreased concentration of the pollutant in roots of intensive Ca-supplied maize plants grown in the environment containing 60 mg Ni/kg. Moreover there was significantly elevated Fe content in highly fertilized with iron spinach plants grown in the presence of 60 mg Ni/kg and in shoots of Ni-treated maize plants intensively supplied with Ca or Fe. Generally, high content of Ca or Fe in the growth medium significantly raised the content of free and bound Ca in shoots of Ni-stressed spinach plants. The same phenomenon was found in roots, but only in the presence of 60 mg Ni. Intensive nutrition supplementation of Ni-treated maize plants with Fe or Ca generally did not change the concentration of free Ca in plant organs, but elevated bound Ca levels in roots was observed. Increased bound Ca content was also found in leaves of maize plants intensive supplied with Ca. Thus, intensive Ca or Fe nutrition presents a promising potential for use in the conditions of Ni contamination by increasing plant growth, reducing Ni translocation from roots to shoots and raising the nutritive value of above-ground parts of spinach and maize plants.

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“…In accordance, GA3 is known to counteract the adverse effects of heavy metals (Cd and Pb) on mitotic index and some metabolic mechanisms, e.g. in bean plants (Mansour and Kamel ). No effect of the single and the triple metal combination on ABA levels was observed both in roots and shoots (Table ), highlighting that AUXs and CKs were the hormones specifically affected by the metals.…”

Section: Discussionmentioning

confidence: 99%

“…Control 1.000 ± 0.047 c 1.000 ± 0.047 a 1.000 ± 0.233 a 1.000 ± 0.387 ab 1.000 ± 0.116 a 1.000 ± 0.261 a 1.000 ± 0.289 ab 1. and some metabolic mechanisms, e.g. in bean plants (Mansour and Kamel 2005). No effect of the single and the triple metal combination on ABA levels was observed both in roots and shoots (Table 1), highlighting that AUXs and CKs were the hormones specifically affected by the metals.…”

Section: Rootsmentioning

confidence: 91%

Correlation between hormonal homeostasis and morphogenic responses in Arabidopsis thaliana seedlings growing in a Cd/Cu/Zn multi‐pollution context

Sofo

Vitti

Nuzzaci

et al. 2013

Physiologia Plantarum

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To date, almost no information is available in roots and shoots of the model plant Arabidopsis thaliana about the hierarchic relationship between metal accumulation, phytohormone levels, and glutathione/phytochelatin content, and how this relation affects root development. For this purpose, specific concentrations of cadmium, copper and zinc, alone or in triple combination, were supplied for 12 days to in vitro growing seedlings. The accumulation of these metals was measured in roots and shoots, and a significant competition in metal uptake was observed. Microscopic analyses revealed that root morphology was affected by metal exposure, and that the levels of trans-zeatin riboside, dihydrozeatin riboside, indole-3-acetic acid and the auxin/cytokinin ratio varied accordingly. By contrast, under metal treatments, minor modifications in gibberellic acid and abscisic acid levels occurred. Real-time polymerase chain reaction analysis of some genes involved in auxin and cytokinin synthesis (e.g. AtNIT in roots and AtIPT in shoots) showed on average a metal up-regulated transcription. The production of thiol-peptides was induced by all the metals, alone or in combination, and the expression of the genes involved in thiol-peptide synthesis (AtGSH1, AtGSH2, AtPCS1 and AtPCS2) was not stimulated by the metals, suggesting a full post-transcriptional control. Results show that the Cd/Cu/Zn-induced changes in root morphology are caused by a hormonal unbalance, mainly governed by the auxin/cytokinin ratio.

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Interactive Effect of Heavy Metals and Gibberellic Acid on Mitotic Activity and Some Metabolic Changes of Vicia faba L. Plants

Cited by 28 publications

References 12 publications

Acclimation of cadmium-induced genotoxicity and oxidative stress in mung bean seedlings by priming effect of phytohormones and proline

Acclimation of cadmium-induced genotoxicity and oxidative stress in mung bean seedlings by priming effect of phytohormones and proline

Growth and Mineral Composition of Nickel-Stressed Plants Under Conditions of Supplementation With Excessive Amounts of Calcium and Iron

Correlation between hormonal homeostasis and morphogenic responses in Arabidopsis thaliana seedlings growing in a Cd/Cu/Zn multi‐pollution context

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