Intercalating methylene blue in molecular beacon for sensitive detection of salivary TNF-α towards early diagnosis of oral cancer

Abstract

Oral cancer is the sixth most common cancer globally, and pro-inflammatory cytokines are associated with oral cancer development and progression. Monitoring of salivary pro-inflammatory cytokines is essential to the early...

Graphene family in cancer therapy: recent progress in cancer gene/drug delivery applications

“…Beacon conformational reconfiguration occurs spontaneously when an MB hybridizes with its complementary target DNA, mRNA, and microRNA, 125 resulting in fluorescence restoration. 126 Many studies have used MBs as early cancer diagnosis agents, 127 which may help cancer therapy by determining specific gene expression in real-time. 128 Graphene derivatives are one of the potential transfer agents for genetic materials.…”

“…Many studies have used MBs as early cancer diagnosis agents, 127 which may help cancer therapy by determining specific gene expression in real-time. 128 Graphene derivatives are one of the potential transfer agents for genetic materials.…”

Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions

“…Label-free electrochemical detection of biomolecules either uses the change in the square wave voltammetry (SWV) reduction peak current of a redox probe or monitors the change in real and imaginary impedance (EIS) during immunorecognition events. Moreover, SWV-EIS transductions are considered more sensitive than commercially available ELISA kits [ 25 , 26 , 27 , 28 ], while amperometric sensing (current signal measurements) [ 29 , 30 , 31 , 32 ] and differential pulse voltammetry (DPV) [ 33 , 34 , 35 ] are considered advantageous as low-cost technologies and in good agreement with ELISA performance. Herein, several electrochemical studies using the Cys/GA reagents are reported and discussed ( Table 2 ).…”