Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA

Chen, Ying; Xiang, Yun; Yuan, Ruo

doi:10.1016/j.aca.2015.07.059

Cited by 16 publications

(7 citation statements)

References 53 publications

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“…Enzymatic amplification strategies have been deployed using rolling circle amplification to form DNAzymes, 78 cyclic exponential amplification recycling, 79 doxorubicin conjugated quantum dots, 80 T7 exonuclease recycling and downstream silver deposition, 81 ECL quenching via Phi29 DNA polymerase mediated strand displacement, 82 and a dual target amplification strategy with combined ECL and fluorescence detection. 83 With one exception, all of these strategies report LODs ranging between 10 fM and 100s of aM with dyamic ranges varying 2–5 orders of magnitude.…”

Section: Biosensor Methodsmentioning

confidence: 99%

Emerging Biosensing Approaches for microRNA Analysis

2015

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Section: Biosensor Methodsmentioning

confidence: 99%

Emerging Biosensing Approaches for microRNA Analysis

2015

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“…Device-based analytical strategies mainly include electrochemical sensors and MEMS [14,15]. Methods-based approaches mainly include fluorescence, colorimetric, and luminescence assays [16,17,18,19,20,21,22,23,24,25,26,27,28]. Among these, the fluorometric method is an excellent choice for the quantitative detection of miRNAs due to its high sensitivity and convenience of operation.…”

Section: Introductionmentioning

confidence: 99%

An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA

Liu

et al. 2017

Sensors

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MicroRNAs (miRNAs) act as biomarkers for the diagnosis of a variety of cancers. Since the currently used methods for miRNA detection have limitations, simple, sensitive, and cost-effective methods for the detection of miRNA are required. This work demonstrates a facile, quencher-free, fluorescence-based analytical method for cost-effective and sensitive detection of miRNA using a super 2-aminopurine (2-AP)-labeled hairpin probe (HP) and exonuclease I activity. Specifically, the fluorescence of 2-AP is strongly quenched when it is incorporated within DNA. In the presence of a target miRNA, HP attains an open conformation by hybridizing with the target miRNA to form a double-stranded structure with a protruding 3′-terminus. Next, the digestion of the protruding 3′-terminus is triggered by exonuclease I, during which 2-AP is released free in solution from the DNA, thereby increasing fluorescence. This method is highly sensitive, with a detection limit of 0.5 nM—10 times lower than a previously reported quencher-free fluorescence method. Furthermore, this method has potential applications in clinical diagnosis and biomedical research.

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“…We chose methylene blue (MB) as the covalent reporter and doxorubicin (DOX) as the intercalated one. As the DOX molecule tends to be intercalated into GC base pairs in the double-stranded nucleic acids, − here we selected GC-rich DNA aptamers with a secondary structure of a double-stranded segment against aminoglycoside and ATP, respectively. More significantly, the binding sites of their targets stand apart from the intercalation site for DOX, promising the sensitivity of these sensors (Figures S1 and S2).…”

Section: Resultsmentioning

confidence: 99%

Employing an Intercalated Redox Reporter in Electrochemical Aptamer-Based Biosensors to Enable Calibration-Free Molecular Measurements in Undiluted Serum

Zhu

et al. 2020

Anal. Chem.

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Electrochemical aptamer-based (E-AB) biosensors suffer from sensor-to-sensor signal variations due to the variation of the total number and the heterogeneity of probes immobilized on the electrode surface, with the former attracting more attention. As such, a calibration process to correct for such variations is required for this type of sensor, causing inconvenience and inaccessibility in harsh sensing environments such as blood samples, which has dramatically limited the widespread clinical use of biosensors. In response, here, we have adopted E-AB sensors to achieve calibration-free measurements of small biological/drug molecules. Specifically, we employ one probe-attached redox reporter and a second intercalated redox reporter to generate two signals, achieving good sensor-to-sensor reproducibility and thus obviating the need for calibration. We first demonstrated the capability of E-AB sensors for the accurate measurement of kanamycin, tobramycin, and adenosine triphosphate (ATP) in phosphate-buffered saline (PBS) buffer, achieving concentration ranges of approximately 4.7 × 103-, 2.0 × 103-, and 12.7-fold, respectively. Then, we applied this calibration-free approach to the measurement of these three target molecules directly in undiluted serum, achieving a concentration precision of a few micromolars.

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Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA

Cited by 16 publications

References 53 publications

Emerging Biosensing Approaches for microRNA Analysis

Emerging Biosensing Approaches for microRNA Analysis

An Exonuclease I-Based Quencher-Free Fluorescent Method Using DNA Hairpin Probes for Rapid Detection of MicroRNA

Employing an Intercalated Redox Reporter in Electrochemical Aptamer-Based Biosensors to Enable Calibration-Free Molecular Measurements in Undiluted Serum

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