Interceptor potential of C60 fullerene aqueous solution: a comparative analysis using the example of the antitumor antibiotic mitoxantrone

C60 Fullerene Clusters Stabilize the Biologically Inactive Form of Topotecan

“…Inspection of the calculated parameters in Table evidences their general compliance with the complexation parameters reported before for other related drug molecules with C 60 fullerene. ,, For instance, the relation K h1 > K h2 noted previously for various ligands (see refs , for details) also holds for the TPT-C 60 case indicating the predominance of the stacking-type complexation over the adsorption into clusters. The double inequality ε h2 > ε m > ε h1 previously reported for doxorubicin and proflavine, appears to be valid for the TPT case as well.…”

“…The titration curve was fitted according to the procedure thoroughly described in ref using the “upscaled” model briefly described in Appendix A. The fitting curve (blue line in Figure B) agrees well with the experimental data (red dots in Figure B) and complies with the values of equilibrium parameters of complexation, calculated in this work and outlined in Table .…”

“…Using the UV/vis titration technique, we found that TPT is effectively adsorbed into large C 60 fullerene clusters, which act as a platform for drug delivery. It is worth noting that such a role of C 60 fullerene clusters is typical for similar molecular systems containing an aromatic compound. − , The preliminary in vitro test on nonproliferating human buccal epithelium cells also demonstrated changes characteristic of such systems. , However, no such effect was observed in five different proliferating cell lines, including the test of the influence of the TPT-C 60 mixture on plasmid DNA relaxation as a biological target for the drug. This result was unexpected mainly because it falls out of the recognized behavior of TPT and C 60 fullerene in separate when they are present in other drug–interceptor combinations with a proven correlation between complexation and its effect in vitro.…”

“…[15][16][17][18]30 The preliminary in vitro test on nonproliferating human buccal epithelium cells also demonstrated changes characteristic of such systems. 19,24 However, no such effect was observed in five different proliferating cell lines, including the test of the influence of the TPT-C 60 mixture on plasmid DNA relaxation as a biological target for the drug. This result was unexpected mainly because it falls out of the recognized behavior of TPT and C 60 fullerene in separate when they are present in other drug−interceptor combinations with a proven correlation between complexation and its effect in vitro.…”

“…Studying the heterochromatization process, one can estimate changes in the functional activity of the cell nucleus. The degree of euchromatin condensation in interphase cell nuclei was assessed by the heterochromatin granule quantity (HGQ) according to the procedure described in detail in refs , . The higher the HGQ value (i.e., the higher the degree of heterochromatin granulation), the lower the transcriptional activity in cell nuclei.…”