Interfacing Antibody-Based Microarrays and Digital Holography Enables Label-Free Detection for Loss of Cell Volume

El-Schich, Zahra; Nilsson, Emmy; Gerdtsson, Anna Sandström; Wingren, Christer; Wingren, Anette Gjörloff

doi:10.4155/fso.14.2

Cited by 8 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the U2932 cell line, the average cell area did not change, whereas the average cell volume significantly decreased after etoposide treatment (Figure 2). Interestingly, the results may indicate cell line sensitivity, which also has been shown in the earlier studies [25,27]. In conclusion, cell death experiments performed with DH microscopy reveal that small differences between two cell lines can be clarified.…”

Section: Cell Death Of Adherent Cellssupporting

confidence: 60%

“…The cell number was stable over time, but the two cell lines used showed changes of cell area and cell irregularity after treatment. The cell volume in etoposide-treated cells was decreased, whereas untreated cells showed stable volume [27]. In conclusion, cell death of suspension cells investigated with the help of antibody-based microarrays and DH demonstrated that morphological parameters can be investigated of different cell lines and treatments.…”

Section: Cell Death Of Suspension Cells On Antibody-based Microarraymentioning

confidence: 98%

“…Antibody-based microarray techniques have been used to determine phenotypic protein expression profiles for human B cell sub-populations [39] and to detect soluble antigens [40]. In our study [27], we combined DH microscopy and antibody-based microarray to introduce a powerful tool to measure morphological changes in specifically etoposide-treated antibody-captured cells, U2932, and Jurkat ( Figure 6). We demonstrated that the cell number, mean area, thickness, and volume could be noninvasively measured by using DH microscopy.…”

Section: Cell Death Of Suspension Cells On Antibody-based Microarraymentioning

confidence: 99%

See 2 more Smart Citations

Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

El-Schich¹,

Kamlund²,

Janicke³

et al. 2017

Holographic Materials and Optical Systems

Self Cite

View full text Add to dashboard Cite

Digital holographic (DH) microscopy is a digital high-resolution holographic imaging technique with the capacity of quantification of cellular conditions without any staining or labeling of cells. The unique measurable parameters are the cell number, cell area, thickness, and volume, which can be coupled to proliferation, migration, cell cycle analysis, viability, and cell death. The technique is cell friendly, fast and simple to use and has unique imaging capabilities for time-lapse investigations on both the single cell and the cell-population levels. The interest for analyzing specifically cell volume changes with DH microscopy, resulting from cytotoxic treatments, drug response, or apoptosis events has recently increased in popularity. We and others have used DH microscopy showing that the technique has the sensitivity to distinguish between different cells and treatments. Recently, DH microscopy has been used for cellular diagnosis in the clinic, providing support for using the concept of DH, e.g., screening of malaria infection of red blood cells (RBC), cervix cancer screening, and sperm quality. Because of its quick and label-free sample handling, DH microscopy will be an important tool in the future for personalized medicine investigations, determining the optimal therapeutic concentration for both different cancer types and individual treatments.

show abstract

Section: Cell Death Of Adherent Cellssupporting

confidence: 60%

Section: Cell Death Of Suspension Cells On Antibody-based Microarraymentioning

confidence: 98%

Section: Cell Death Of Suspension Cells On Antibody-based Microarraymentioning

confidence: 99%

See 1 more Smart Citation

Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

El-Schich¹,

Kamlund²,

Janicke³

et al. 2017

Holographic Materials and Optical Systems

Self Cite

View full text Add to dashboard Cite

show abstract

“…DHC applications have previously been used to discriminate between the maturity levels of red blood cells, perform cell death studies and analyze drug responses [23,[37][38][39]. Cell optical area, thickness and volume are useful parameters, particularly for discrimination between different cell types [39][40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

El-Schich

Janicke²,

Alm³

et al. 2020

Applied Sciences

Self Cite

View full text Add to dashboard Cite

Breast cancer is the second most common cancer worldwide. Metastasis is the main reason for death in breast cancer, and today, there is a lack of methods to detect and isolate circulating tumor cells (CTCs), mainly due to their heterogeneity and rarity. There are some systems that are designed to detect rare epithelial cancer cells in whole blood based on the most common marker used today, the epithelial cell adhesion molecule (EpCAM). It has been shown that aggressive breast cancer metastases are of non-epithelial origin and are therefore not always detected using EpCAM as a marker. In the present study, we used an in vitro-based circulating tumor cell model comprising a collection of six breast cancer cell lines and white blood cell lines. We used digital holographic cytometry (DHC) to characterize and distinguish between the different cell types by area, volume and thickness. Here, we present significant differences in cell size-related parameters observed when comparing white blood cells and breast cancer cells by using DHC. In conclusion, DHC can be a powerful diagnostic tool for the characterization of CTCs in the blood.

show abstract

“…Although for fixed applications, suspension cells may be attached to coverslips by cytocentrifugation such as cytospin technology 55 , a live-cell imaging setup is normally not compatible with suspension cells. DHM has previously been performed on live suspension cells immobilized by antibody capture 56 . Here, we tested the option to use thin 3D gels to immobilize suspension cells for multifield monitoring.…”

Section: Dhm Analysis Of Suspension Cells Embedded In Matrigel Can Qumentioning

confidence: 99%

Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays

Hellesvik

Øye

Aksnes

2020

Sci Rep

View full text Add to dashboard Cite

3D cell culture assays are becoming increasingly popular due to their higher resemblance to tissue environment. These provide an increased complexity compared to the growth on 2D surface and therefore allow studies of advanced cellular properties such as invasion. We report here on the use of 3D Matrigel cell preparations combined with a particular gentle and informative type of live-cell microscopy: quantitative digital holographic microscopy (DHM), here performed by a commercial software-integrated system, currently mostly used for 2D cell culture preparations. By demonstrating this compatibility, we highlight the possible time-efficient quantitative analysis obtained by using a commercial software-integrated DHM system, also for cells in a more advanced 3D culture environment. Further, we demonstrate two very different examples making use of this advantage by performing quantitative DHM analysis of: (1) wound closure cell monolayer Matrigel invasion assay and (2) Matrigel-trapped single and clumps of suspension cells. For both these, we benefited from the autofocus functionality of digital phase holographic imaging to obtain 3D information for cells migrating in a 3D environment. For the latter, we demonstrate that it is possible to quantitatively measure tumourigenic properties like growth of cell clump (or spheroid) over time, as well as single-cell invasion out of cell clump and into the surrounding extracellular matrix. Overall, our findings highlight several possibilities for 3D digital holographic microscopy applications combined with 3D cell preparations, therein studies of drug response or genetic alterations on invasion capacity as well as on tumour growth and metastasis.

show abstract

Interfacing Antibody-Based Microarrays and Digital Holography Enables Label-Free Detection for Loss of Cell Volume

Cited by 8 publications

References 34 publications

Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays

Contact Info

Product

Resources

About