Interfacing Pathogen Detection with Smartphones for Point-of-Care Applications

Ding, Xiong; Mauk, Michael G.; Yin, Kun; Kadimisetty, Karteek; Liu, Changchun

doi:10.1021/acs.analchem.8b04973

Cited by 121 publications

(85 citation statements)

References 155 publications

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“…We then set out to demonstrate the use of the cell STORM II system for advanced biological research questions. Antibody-body based microscopic detection of pathogens or infected cells remains an important diagnostic tool [55]. In addition, there is a need for high resolution microscopy in many labs working with viruses and other pathogens.…”

Section: Versatile Tool For Multimodal In-vitro Tirf Imaging In Biolomentioning

confidence: 99%

Nanoscopy on the Chea(i)p

Diederich

Helle

Then

et al. 2020

Preprint

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Super-resolution microscopy allows for stunning images with a resolution well beyond the optical diffraction limit, but the imaging techniques are demanding in terms of instrumentation and software. Using scientific-grade cameras, solid-state lasers and top-shelf microscopy objective lenses drives the price and complexity of the system, limiting its use to well-funded institutions. However, by harnessing recent developments in CMOS image sensor technology and low-cost illumination strategies, super-resolution microscopy can be made available to the mass-markets for a fraction of the price. Here, we present a 3D printed, self-contained super-resolution microscope with a price tag below 1000 $ including the objective and a cellphone. The system relies on a cellphone to both acquire and process images as well as control the hardware, and a photonic-chip enabled illumination. The system exhibits 100nm optical resolution using single-molecule localization microscopy and can provide live super-resolution imaging using light intensity fluctuation methods. Furthermore, due to its compactness, we demonstrate its potential use inside bench-top incubators and high biological safety level environments imaging SARS-CoV-2 viroids. By the development of low-cost instrumentation and by sharing the designs and manuals, the stage for democratizing super-resolution imaging is set.

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Section: Versatile Tool For Multimodal In-vitro Tirf Imaging In Biolomentioning

confidence: 99%

Nanoscopy on the Chea(i)p

Diederich

Helle

Then

et al. 2020

Preprint

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“…Over the past decade, these smartphone-based technologies have been widely used for the detection of different pathogens [ 57 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 ]. A unique fluorescence-based approach of combining quantum dot barcode technology with smartphones was used to detect HIV and Hepatitis-B virus [ 131 ].…”

Section: Smartphone-based Detection Systemmentioning

confidence: 99%

Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices

et al. 2020

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Infectious diseases caused by bacteria and viruses are highly contagious and can easily be transmitted via air, water, body fluids, etc. Throughout human civilization, there have been several pandemic outbreaks, such as the Plague, Spanish Flu, Swine-Flu, and, recently, COVID-19, amongst many others. Early diagnosis not only increases the chance of quick recovery but also helps prevent the spread of infections. Conventional diagnostic techniques can provide reliable results but have several drawbacks, including costly devices, lengthy wait time, and requirement of trained professionals to operate the devices, making them inaccessible in low-resource settings. Thus, a significant effort has been directed towards point-of-care (POC) devices that enable rapid diagnosis of bacterial and viral infections. A majority of the POC devices are based on plasmonics and/or microfluidics-based platforms integrated with mobile readers and imaging systems. These techniques have been shown to provide rapid, sensitive detection of pathogens. The advantages of POC devices include low-cost, rapid results, and portability, which enables on-site testing anywhere across the globe. Here we aim to review the recent advances in novel POC technologies in detecting bacteria and viruses that led to a breakthrough in the modern healthcare industry.

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“…Recently, there has been a major push to use smartphone technology for infectious disease detection instead of expensive equipment, enabling smartphone-based point-of-care diagnostic applications 22 . For example, Song et al 23 combined the bioluminescent real time reporter (BART) of LAMP with a smartphone to rapidly quantify Zika virus and spatiotemporally map the disease.…”

Section: Introductionmentioning

confidence: 99%

Cleavable hairpin beacon-enhanced fluorescence detection of nucleic acid isothermal amplification and smartphone-based readout

Ding

Yin

et al. 2020

Sci Rep

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Fluorescence detection of nucleic acid isothermal amplification utilizing energy-transfer-tagged oligonucleotide probes provides a highly sensitive and specific method for pathogen detection. However, currently available probes suffer from relatively weak fluorescence signals and are not suitable for simple, affordable smartphone-based detection at the point of care. Here, we present a cleavable hairpin beacon (CHB)-enhanced fluorescence detection for isothermal amplification assay. The CHB probe is a single fluorophore-tagged hairpin oligonucleotide with five continuous ribonucleotides which can be cleaved by the ribonuclease to specifically initiate DNA amplification and generate strong fluorescence signals. By coupling with loop-mediated isothermal amplification (LAMP), the CHB probe could detect Borrelia burgdorferi (B. burgdorferi) recA gene with a sensitivity of 100 copies within 25 min and generated stronger specific fluorescence signals which were easily read and analysed by our programmed smartphone. Also, this CHB-enhanced LAMP (CHB-LAMP) assay was successfully demonstrated to detect B. burgdorferi DNA extracted from tick species, showing comparable results to real-time PCR assay. In addition, our CHB probe was compatible with other isothermal amplifications, such as isothermal multiple-self-matching-initiated amplification (IMSA). Therefore, CHB-enhanced fluorescence detection is anticipated to facilitate the development of simple, sensitive smartphone-based point-of-care pathogen diagnostics in resource-limited settings.

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Interfacing Pathogen Detection with Smartphones for Point-of-Care Applications

Abstract: Graphcial Abstract

Cited by 121 publications

References 155 publications

Nanoscopy on the Chea(i)p

Nanoscopy on the Chea(i)p

Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices

Cleavable hairpin beacon-enhanced fluorescence detection of nucleic acid isothermal amplification and smartphone-based readout

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