Interference by haem in the application of the thiobarbituric acid test

Cowlishaw, B.

doi:10.1016/0006-3002(61)90258-x

Cited by 6 publications

(2 citation statements)

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“…However, the application of the assay is limited in many cases. TBA is known to form coloured complexes not only with MDA but also with carbohydrates, pyrimidines, amino acids, aliphatic aldehydes, haem-containing substances and other compounds (Barber and Bernheim, 1967;Cowlishaw, 1961;Draper and Hadley, 1990;Esterbauer and Cheeseman, 1990;Gutteridge and Halliwell, 1990). Previously, we have found that TBA reagent applied to plant tissues produces a number of products absorbing between 400 and 600 nm which can be separated chromatographically.…”

Section: Introductionmentioning

confidence: 99%

“…, , 1979. In pea roots, under the conditions of the TBA test, a product with an absorbance maximum at 551 nm (see also Cowlishaw, 1961;Halliwell and Gutteridge, 1989) overlaps the absorption band of the MDA-TBA complex (Merzlyak et ul., 1978). In addition, many plant tissues contain anthocyanins and other water-soluble pigments that have a strong absorption in acidic solutions.…”

Section: Introductionmentioning

confidence: 99%

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Assay of the thiobarbituric acid‐reactive products of lipid peroxidation in plants using derivative absorption spectroscopy

Merzlyak

Zhigalova

Shevyryova

1992

Phytochemical Analysis

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The spectrophotometric determination of 2‐thiobarbituric acid‐reactive products of lipid peroxidation in plants is limited because of the presence of substances forming coloured complexes with the reagent as well as water‐soluble pigments. In order to overcome this interference 2nd derivative analysis of the absorption spectra was applied. The results of the study indicate that the method can be performed using cotton and maple leaves that contain high amounts of anthocyanins and bifunctional aldehydes, respectively. The accumulation of lipid peroxidation products was observed in cotton plants treated with the herbicide diquat. In wheat roots the band of malondialdehyde‐thiobarbituric acid absorption was not resolved either in intact or in diquat‐treated plants, because of the production of a chromophore with an absorption maximum at 551 nm. The sensitivity of the 2nd derivative determination of malondialdehyde is about 0.2 μ. A linear response was observed up to 8–10 μ of the aldehyde.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%