Interference Expression of StMSD Inhibited the Deposition of Suberin and Lignin at Wounds of Potato Tubers by Reducing the Production of H2O2

Abstract

Superoxide dismutase (SOD) actively participates in the wound stress of plants. However, whether StMSD mediates the generation of H2O2 and the deposition of suberin polyphenolic and lignin at potato tuber wounds is elusive. In this study, we developed the StMSD interference expression of potato plants and tubers by Agrobacterium tumefaciens-mediated transformation. The StSOD expression showed a marked downregulation in StMSD-interference tubers, especially StCSD2 and StCSD3. The content of O2•− exhibited a noticeable increase together with the inhibition in H2O2 accumulation. Moreover, the gene expression levels of StPAL (phenylalanine ammonia-lyase) and StC4H (cinnamate-4-hydroxylase) were downregulated in StMSD-interference tubers, and less suberin polyphenolic and lignin depositions at the wounds were observed. Taken together, the interference expression of StMSD can result in less suberin polyphenolic and lignin deposition by inhibiting the disproportionation of O2•− to H2O2 and restraining phenylpropanoid metabolism in tubers.

Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination

“…In addition, cinnamic acid was rapidly converted to trans-2-hydroxycinnamate under the catalysis of cinnamate 2-hydroxylase, followed by the generation of coumarine through the upregulation of β-glucosidase. Studies have shown that upregulation of PAL , C4H , and 4CL in plants can increase plant tolerance to biotic or abiotic stress [ 49 , 50 ]. Among them, overexpression of PAL has been proven to reduce the level of ROS in Arabidopsis seeds and leaves, increase the activity of POD, CAT, and the content of flavonoids, and reduce the accumulation of MDA [ 51 ].…”