Interference reduction isothermal nucleic acid amplification strategy for COVID-19 variant detection

Li, Guodong; Ko, Chung-Nga; Wang, Zikang; Chen, Feng; Wang, Wanhe; Ma, Dik‐Lung; Leung, Chung‐Hang

doi:10.1016/j.snb.2022.133006

Cited by 2 publications

(2 citation statements)

References 29 publications

(36 reference statements)

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“…76,77 It enables rapid and accurate detection of pathogenic nucleic acids, including viruses, bacteria, and fungi, making it crucial for early diagnosis of infectious diseases and epidemic surveillance. 78,79 Additionally, ITA can be used for detecting gene mutations and genetic disorders, providing important support for personalized medicine. 80,81 Common ITA techniques include Loop-mediated isothermal amplification (LAMP), Recombinase Polymerase Amplification (RPA), Strand Displacement Amplification (SDA), and helicase-dependent amplification (HDA), each with its own characteristics and applicable range.…”

Section: Nucleic Acid Isothermal Amplificationmentioning

confidence: 99%

“…ITA has found wide applications in fields such as molecular biology, medical diagnostics, and environmental monitoring, presenting numerous advantages 76,77 . It enables rapid and accurate detection of pathogenic nucleic acids, including viruses, bacteria, and fungi, making it crucial for early diagnosis of infectious diseases and epidemic surveillance 78,79 . Additionally, ITA can be used for detecting gene mutations and genetic disorders, providing important support for personalized medicine 80,81…”

Section: Molecular Detection Technologymentioning

confidence: 99%

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Intelligent point of care testing for medicine diagnosis

Gao,

2024

Interdisciplinary Medicine

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Intelligent point‐of‐care testing (iPOCT) is a rapidly advancing technology that combines molecular detection and bio‐sensing techniques to achieve instant and accurate analysis through advanced sensors and intelligent algorithms. Molecular detection technologies, such as nucleic acid isothermal amplification, biochip and microfluidics, enable rapid analysis of the chemical composition and concentration of samples, thereby determining their presence. Bio‐sensing technology utilizes the recognition properties of biomolecules in conjunction with sensors to detect specific biomolecules or biological processes. iPOCT technology holds broad prospects for application in the field of healthcare and is expected to enhance detection sensitivity, accuracy, and intelligence through further development. In this review, we delineate the developmental journey of iPOCT, followed by a discussion on the principles of molecular detection and bio‐sensing technology, as well as the design of iPOCT devices for health monitoring and disease diagnosis. Furthermore, we showcased illustrative examples of iPOCT applications in disease diagnosis, highlighting the integration of iPOCT technology with wearable devices and smartphones for attaining more precise and personalized diagnostic results.

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Section: Nucleic Acid Isothermal Amplificationmentioning

confidence: 99%

Section: Molecular Detection Technologymentioning

confidence: 99%

Intelligent point of care testing for medicine diagnosis

Gao,

2024

Interdisciplinary Medicine

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Light Harvesting Nanoprobe for Trace Detection of Hg2+ in Water

et al. 2023

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The continuously increasing flow of toxic heavy metals to the environment due to intensive industrial activity and tightening requirements with regard to the content of metal ions in drinking and discharged waters urges the development of affordable and sensitive devices to the field control of pollutants. Here, we report a new thiated Rhodamine-lactam probe for Hg2+ detection and demonstrate how its sensitivity can be increased via the incorporation of the probe molecules into the optically transparent siloxane-acrylate coatings on polymethyl methacrylate and, alternatively, into the water-dispersible light-harvesting FRET nanoparticles (NPs), in which dye cations are separated by fluorinated tetraphenylborate anions. We have shown that the optimization of the FRET NPs composition had allowed it to reach the antenna effect of ~300 and fabricate “off/on” sensor for Hg2+ ion determination in aqueous solutions with the detection limit of ~100 pM, which is far below the maximum permissible concentration (MPC) of mercury in drinking water recommended by the World Health Organization. Although this work is more proof-of-concept than a ready-to-use analytical procedure, the suggested approaches to fabrication of the FRET NPs based on the popular rhodamine-lactam platform can be used as a background for the development of low-cost portable sensing devices for the extra-laboratory determination of hazardous metal ions.

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Interference reduction isothermal nucleic acid amplification strategy for COVID-19 variant detection

Cited by 2 publications

References 29 publications

Intelligent point of care testing for medicine diagnosis

Intelligent point of care testing for medicine diagnosis

Light Harvesting Nanoprobe for Trace Detection of Hg2+ in Water

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