Interferometric detection of microRNAs using a capillary optofluidic sensor

Liang, Lili; Jin, Long; Ran, Yang; Sun, Li-Peng; Guan, Bai‐Ou

doi:10.1016/j.snb.2016.09.153

Cited by 52 publications

(33 citation statements)

References 63 publications

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“…Therefore, by interacting with the evanescent field of the optical microfiber, the plasmonic electromagnetic field of Ag NPs on nanointerface clearly increased the energy density at the microfiber surface . Because the sensitivity of optical microfiber is proportional to the surface binding‐induced change in propagation constants (Δβ) and Δβ is proportional to the evanescent field of microfiber ( E ), as expressed by Equation , the sensitivity is proportional to the surface energy of the microfiber

Sensitivity = k_{1} • Δ β = k_{2} • E (x, y)

where k 1 and k 2 are constant coefficients.…”

Section: Resultsmentioning

confidence: 99%

Real‐Time Cellular Cytochrome C Monitoring through an Optical Microfiber: Enabled by a Silver‐Decorated Graphene Nanointerface

Huang

Chen

et al. 2018

Advanced Science

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The translocation of cytochrome c (cyt c) from mitochondria and out of cell is an important signal of cell apoptosis. Monitoring this process extracellularly without invasion and cytotoxicity to cells is of great importance to understand certain diseases at the cellular level; however, it requires sensors with ultrahigh sensitivity and miniature size. This study reports an optical microfiber aptasensor with a silver‐decorated graphene (Ag@RGO) nanointerface for real‐time cellular cyt c monitoring. Owing to an interfacial sensitization effect coupled with the plasmonic electromagnetic enhancement of silver nanoparticles and chemical enhancement of graphene platforms, which enhances the energy density on microfiber surface obviously, the lowest limit of detection achieved is 6.82 × 10−17 m, which is approximately five orders of magnitude lower than those of existing methods. This microfiber successfully detects the ultralow concentrations of cyt c present during the initial stage of apoptosis in situ. As the microfiber functionalized by Ag@RGO nanointerface can be varied to meet any specific detection objective, this work opens up new opportunities to quantitatively monitor biological functions occurring at the cellular level.

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Sensitivity = k_{1} • Δ β = k_{2} • E (x, y)

where k 1 and k 2 are constant coefficients.…”

Section: Resultsmentioning

confidence: 99%

Real‐Time Cellular Cytochrome C Monitoring through an Optical Microfiber: Enabled by a Silver‐Decorated Graphene Nanointerface

Huang

Chen

et al. 2018

Advanced Science

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“…In this article, we present a 23.33333pt×3.33333pt2 optical fiber coupler with a solid core fiber (SCF) and HCF. The coupler was fabricated using hydrogen flame-based process [35,36]. HCF has a silica layer of RI similar to the cladding RI of a regular SCF, which imparts light-guiding capabilities.…”

Section: Introductionmentioning

confidence: 99%

Hollow Fiber Coupler Sensor

Kuruba

Lü

2019

Sensors

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We present a bi-conical optical directional coupler composed of solid and hollow core fibers. Through an evanescent wave coupling mechanism, the detection of liquid refractive index and its temperature are demonstrated. The experimental results illustrated that the sensor offers a sensitivity of 4.03 ± 0.50 volts per refractive index units (V/RIU) for refractive indices ranging from 1.331 ± 0.003 to 1.403 ± 0.003 with a resolution of 3.5 × 10 − 3 refractive index units (RIU).

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“…In contrast, in the middle area, the transmission loss sensitivity of determination was 27.352 dB/log ng/mL, the sensitivity and coefficient of the wavelength shift were 67.539 dB/log ng/mL and 0.939, respectively, indicating excellent sensitivity to variations in the Mahlavu specimen concentration (as shown in Figure S5c). Compared with the reported works [28,30,31] (as shown in Table S1), the detection limits and detection range have improved. We also finished a three-cycle experiment to detect the Mahlavu fragment.…”

Section: Resultsmentioning

confidence: 74%

“…Their results showed a detection limit of 0.001 nm/(µg/mL) and detection range of 0 to 10 µg/mL. Liang et al [31] demonstrated a microfiber-capillary optofluidic sensor for miRNA-let7a detection based on the interference of optical modes. The optofluidic sensor was fabricated by aligning a microfiber in lateral contact with a capillary to form a modal interferometer.…”

Section: Introductionmentioning

confidence: 99%

A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA

Wen¹,

Huang²,

Li³

et al. 2020

Sensors

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This study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticles and miRNA-133a probe onto the silicon dioxide of the fiber surface. For Mahlavu of hepatocellular carcinoma (HCC), detection is determined by the wavelength shift and transmission loss of a U-shaped optical fiber biosensor. The spectral sensitivity of wavelength and their coefficient of determination are found at −218.319 nm/ ng/mL and 0.839, respectively. Concurrently, the sensitivity of transmission loss and their coefficient of determination are found at 162.394 dB/ ng/mL and 0.984, respectively. A method for estimating the limit of detection of Mahlavu is at 0.0133 ng/mL. The results show that the proposed U-shaped biosensor is highly specific to miRNA-133a and possesses good sensitivity to variations in specimen concentration. As such, it could be of substantial value in microRNA detection.

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Interferometric detection of microRNAs using a capillary optofluidic sensor

Cited by 52 publications

References 63 publications

Real‐Time Cellular Cytochrome C Monitoring through an Optical Microfiber: Enabled by a Silver‐Decorated Graphene Nanointerface

Real‐Time Cellular Cytochrome C Monitoring through an Optical Microfiber: Enabled by a Silver‐Decorated Graphene Nanointerface

Hollow Fiber Coupler Sensor

A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA

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